...across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials ...perceived signals to physical actions. Understanding neuronal pathways and the function of certain cells depends...be directed to specific types of cells, such as neuron subtypes, thanks to viral serotype specificity ...available VSDs has been successful even at the single neuron level in vitro. In vivo monitoring has also been...voltage is particularly physiologically relevant in neurons, myocytes, and endocrine cells. It follows that...voltage indicators advance their applications in neuroscience. Curr Opin Biomed Eng. 2019. 12:111-117. doi...

...Lee and Pantelis Tsoulfas of the Department of Neurological Surgery at the University of Miami. The beginning...light microscopy, particularly related to the neurosciences.  These developments in microscopy coupled with...of transgenic mice in which a subpopulation of neurons are brightly labeled with GFP (e.g. Thy1-YFP-H ...LSFM with multiple tissue clearing strategies and neuronal labeling methods will greatly aid our understanding...331–336. Pubmed. P. Keller, M.B. Ahrens.  (2015) Neuron, 85, 462-483. Pubmed. E.H.K. Stelzer. (2015). Nature...H. Lee, A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. ...

...could identify the ancestry of the cell lines (European, East Asian, Indigenous American, Africa, or South...lines used in DepMap CRISPR screens are of either European or East Asian ancestry, Sean and Aaron knew that...dependencies that were clearly associated with European or East Asian ancestry groups, far more than expected...human population. Rather, it primarily reflects European ancestry.  Understanding the impact Fig...to better understand how the use of a primarily European reference genome could affect their analysis. ... by the Cancer Dependency Map) than lines from European or East Asian ancestries. These SNPs, however,...

...unable to reach all regions of the CNS. PHP.B, a neurotropic variant of AAV9, is better than AAV9 at gaining...receptor used by PHP.B viruses. Creating PHP.B, a neurotropic AAV9 variant PHP.B’s specificity for C57BL/6J... and transduces the majority of astrocytes and neurons across multiple CNS regions. PHP.eB, an enhanced...PMID: 30819613. Hordeaux, Juliette, et al. "The neurotropic properties of AAV-PHP. B are limited to C57BL...transduction efficiency in the marmoset brain." Neuroscience letters 665 (2018): 182-188. PubMed PMID: 29175632...central and peripheral nervous systems." Nature neuroscience 20.8 (2017): 1172. PubMed PMID: 28671695. PubMed...

...When neuroscience graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of...now available in Addgene’s repository for use in neurons, both in culture and in living brain tissue. “The... the LITEs to control gene expression in mouse neurons and in the brains of living mice both quickly and...clear application to anyone with an interest in neuroscience, their use is by no means limited to particular... want to apply LITEs for the transformation of neurons, she offers a tip: save yourself some time by making...

...various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The ...catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop a biosensor...MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved sensors...

...for neuronal systems. To develop a new single-component tool for magnetogenetic control of neurons, the...pTALYM4 Boris Greber pTAL7a, pTAL7b Michal Zurovec pBlue-TAL Nathan Lawson pJDS Series   Superglue...McMahon et al., report using as few as 150 fly neurons as their starting material! Thus, TRIBE can be ...non-invasive and can rapidly and reversibly stimulate neurons. Recent studies have designed multi-component actuators...finding that it could increase the activity of neurons from brain slice preparations. Magneto2.0 was also...Magneto 2.0 allows for magnetic remote control of neuronal activation. Find Magneto 2.0 plasmids by following...following the links below. Wheeler M, et al. Nat. Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid...

...Detection of Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ... B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before/after application...dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and caption adapted from Kagiampaki et...

...detect the neurotransmitters and other bioactive molecules is integral to the study of neuroscience. To this...improved detection for tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See ...chemogenetic AAV for activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab...with Cre for intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV...

... Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969-4.   Increased neuronal expression...flexibility for in vivo proximity labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution...found at least two variants that display increased neuronal expression in the brain while having decreased...M-CREATE plasmids here!  Goertsen D, et al. Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969...

...29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids...have been successfully transdifferentiated into neurons and β-cells [40-42]. Reprogramming and transdifferentiation...S.C. Zhang, Directed differentiation of dopamine neurons from human pluripotent stem cells. Methods Mol ...human pluripotent stem cells to cerebral cortex neurons and neural networks. Nat Protoc, 2012. 7(10): p...pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: p. 9232. PubMed PMID: 25782665...Direct conversion of fibroblasts to functional neurons by defined factors. Nature, 2010. 463(7284): p....

...the data is heavily dominated by the U.S. (42%), Europe (23%), and China (14%). Spikes in plasmid requests...majority of our plasmid orders come from the U.S. and Europe, we see a corresponding dip in plasmid request ...following hierarchical model: Because the U.S., Europe, and China account for over 75% of all of our plasmid...(the last date in our dataset) for the U.S. and Europe. To improve the stability of our model, we considered...scientists placing orders per day across regions, with Europe most impacted and China least impacted. The inferred...

...far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362, 109314 (2021). Yu, D. et... Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living mouse. Image courtesy...(2015). Shcherbakova, D.M., Stepanenko, O.V., Turoverov, K.K. & Verkhusha, V.V. Near-Infrared Fluorescent...improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun 5, 3626 (...

...fluorescent tags in neurons. Their toolkit named ORANGE (Open Resource for the Application of Neuronal Genome Editing...CRISPR/Cas9-based toolkit for epitope tagging in neurons Harold MacGillavry’s lab developed a CRISPR-based...targeted genomic integration of fluorescent tags in neurons.  Read the paper in bioRxiv Find the ORANGE toolkit...

... in Frontiers in Neuroanatomy and summarized some considerations for precise neuronal targeting using ...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources...

...ion-based communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural...you hear the phrase “action potential?” Firing neurons are likely the first thing that comes to mind. ...exhibits an inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of ...

...Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the... Fig. 1: Antibody production methods    NeuroMab and others have an extensive library of monoclonal...mouse tissue and compare the IHC results to both NeuroMab and the Allen Brain Atlas. The comparison with...intuitive guide for checking their staining and neuroanatomy without the fear of referencing a wrong section...

...Since the pioneering days of Golgi and Cajal, neuroscientists have developed and refined many tools to tease...understand the complex interworking of the 86 billion neurons in the human brain. But studying synaptic connections...generally very small, and can contain different neurotransmitters. Using genetically encoded fluorescent proteins...inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous synaptic proteins...

...in green monkeys and cultured human neurons, with broader neuronal expression than AAV9. This new capsid...https://doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate...-brain barrier and delivering a transgene, with neuron bias observed in young macaques and vascular bias...

...channelrhodopsin for activation of CNS neurons. Current protocols in neuroscience / editorial board, Jacqueline... Simon. The surgeries and standard molecular neuroscience validation experiments we discussed last week...optogenetic molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures...