...started: Determine what type of outcome you are trying to achieve: Do you want to permanently knock out...to activate or repress gene expression? Are you trying to create a single point mutation? Do you want ...

...Experiments tend to be equally complex with users trying to probe multiple targets in parallel. In these...cross-reactivity. To further limit species cross-reactivity, try to find secondary antibodies that are all derived...Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. 977–983). ...

...and to get their answers to the questions. Don't try to get all panelists on the phone at once – you won't...knows some of the people in the audience. I always try to meet audience members before a panel discussion...

...to verify plasmids or validating your new clones, try these tips to make the most of your time and sequence... a number of sequence alignment tools available. Try these resources for lists of alternatives to BLAST...databases available are unlikely to have an exact entry for your favorite gene fused to an epitope tag or...

... maybe even picked up a new technique or tool to try in your own work!) If you've got a research story...

...instead of incubating at -20°C for 1 hour, you can try incubating at -80°C overnight. Centrifuge for 20 ...

...and doing everything, it can be annoying when we try to use them in an experiment and they don't do anything...during replication. To avoid genomic rearrangements, try amplifying the viral vector with bacteria designed...adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. PubMed PMID: 12050007. 7...

...molecular biology tool kit. Consider giving it a try next time you’re screening for positive clones! ...the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional...

...much easier to go where your audience is than to try to convince your audience to come to you (a TikTok... project like a hot potato or burn yourself out trying to slog through unappealing work in your precious...used in all countries; not all areas of any given country have equal access to high-speed internet. Before... Written works Low Flexible. Low barrier to entry. Ample distribution channels both digitally and ...discouraging to build audience. Need space & higher entry barrier for audience versus passive digital consumption...combine multiple types of media. High barrier to entry (people have to enter a building or exhibit). No...video. Easy to target audiences. Low barrier to entry for both audience and communicators. Well-developed...

...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for an...

...modified alleles). For this reason, many laboratories try to enhance HDR by synchronizing the cells, since ...and context-dependent, it might be beneficial to try multiple CRISPR strategies when setting up your experimental.... This limitation is of particular concern when trying to edit a gene using homology directed repair (...identify the genomic sequence for the gene you are trying to target. However, the exact region of the gene...associated with the locus can be identified by mass spectrometry (proteins), RNA-sequencing (RNAs), and NGS (...265-271. PMID: 29431739 Base editing: precision chemistry on the genome and transcriptome of living cells...

...protect the UV box from being cut by the razor blade. Try to get as little excess gel around the band as possible...

...protocols, and, of course, plasmids. Today we’re trying a new way to present plasmid info with a new segment...

...great place to start if you’re new to the topic. Or, try the search bar Now, you can search for the articles...

...less resources and are particularly useful when trying to screen a limited number of cells like primary... plays a significant role in library efficiency. Try the Dolcetto library! Now also available as a lentiviral...

... and should be designed accordingly. If you are trying activate a gene, you should design a control that...that shows maximal activation. Likewise if you are trying to repress a gene, your control might be a system...

... writing a review, or if you’re currently stuck trying to write one, hopefully this post will help you... or recent review exists. Once you pick a topic, try to be specific about exactly what aspect of the field...

...Recombines with the entry clone to generate an expression clone   Ready to try out Gateway cloning?...Press). Additional Resources on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction...to generate entry clones Entry vector attL sites for recombination Used to generate entry clones by TOPO...1: Generate an Entry Clone There are a few different ways to generate our desired entry clone - human ... an attL-containing entry vector.   Figure 4: Method B to create an entry clone: TOPO cloning ... an attL-containing entry vector.   Figure 5: Method C to create an entry clone: Restriction cloning...an attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for...

...your data, too!   Using the Data Hub If you're trying to decide which AAV is right for your experiment...

...select a new antibody using the above process and try again. In some cases, it may be preferable to buy...Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting - used to...immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use labeled antibodies... one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can refer to both...both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (also called dyes...Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows you...capture, and sort single cells. Includes: Flow Cytometry Read on to learn more about the applications common...