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Showing: 81 - 100 of 103 results

  1. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...to dimerization pcDNA3-CFP - Mammalian Expression pCAG-CFP - Mammalian Expression Cerulean 433 475 27 4.7... dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian ...530 68 5.9 Monomer pJL-mGold - Yeast Expression pCaggs-mGold - Mammalian Expression mCitrine 516 529 59... Mammalian Expression DsRed2 563 582 24 Tetramer pCAG-DsRed2 - Mammalian Expression DsRed2-N1 - Mammalian... - Mammalian Expression HcRed1 588 618 0.3 Dimer pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...ataxia Bruce Tempel 30137 pCAX APP 695 APP CAG Alzheimer's Dennis Selkoe 30138 pCAX APP 751 APP CAG Alzheimer's...Dennis Selkoe 30143 pCAX APP delta CT APP CAG Alzheimer's Dennis Selkoe 30144 pCAX APP AENATA APP CAG ...Dennis Selkoe 30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP C99 APP CAG Alzheimer's...Dennis Selkoe 30147 pCAX APPs-695-alpha APP CAG Alzheimer's Dennis Selkoe 30149 pCAX APPs-751-alpha APP...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...APP CAG Alzheimer's Dennis Selkoe 30154 pCAX FLAG APP APP Flag CAG Alzheimer's Dennis Selkoe 31255 pGEX...pScarlet-Kif5a KIF5A mScarlet CMV ALS Beverly Koller 118745 pCAG-Scarlet-Kif5a KIF5A mScarlet CAG ALS Beverly Koller...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target...Researchers have expanded the CRISPR field beyond SpCas9, moving us closer to being able to target every... Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs...

  4. Church Lab CRISPR Plasmids

    Type
    Collection
    ...spectinomycin 48668 M-SPcas Mammalian S. pyogenes Cas9 expression, human optimized 48645 DS-SPcas Bacterial S. ...ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48657 DS-SPcasN- Bacterial nuclease-null SP Cas9 expression 48659...

  5. Chemogenetics Plasmids

    Type
    Collection
    ...Sternson 119739 pCAG PSAM4 GlyR IRES EGFP PSAM4-GlyR CAG IRES-EGFP No Sternson 119740 pCAG PSAM4-5HT3 HC...Psam4GlyR m3m4 HA PSAM4-GlyR m3m4 Syn No Sternson 123066 pCAG PSAM4 5HT3 LC IRES GFP PSAM4-5HT3 LC CAG IRES-EGFP...

  6. CRISPR Plasmids - Tagging

    Type
    Collection
    ... AAVS1 genomic safe harbor locus using untagged SpCas9 (Addgene 41815 or #44719 ) in combination with ...using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which ... integrating at the AAVS1 "safe harbor" locus: eSpCas9(1.1)_No_FLAG_AAVS1_T2 Doyon Lab TAP Tagging Protocol...

  7. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... NLS YFP IX Nucleus NLS YFP Connie Cepko 158000 pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois...FUmGW Membrane Palmitoylation GFP Connie Cepko 14757 pCAG-mGFP Membrane Palmitoylation sequence from GAP43...Actin Filaments LifeAct mCherry Robin Shaw 21948 pCAG-mGFP-Actin Actin Filaments beta-actin EGFP Ryohei...

  8. Retrovirus Plasmids

    Type
    Collection
    ...35617 pCAG-Eco Envelope Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG ...

  9. Lentivirus Plasmids

    Type
    Collection
    ...35617 pCAG-Eco N/A Envelope Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG...

  10. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230)....allows for the simultaneous expression of sgRNA and SpCas9, but does not contain markers for selection 1 ....

  11. Immunology Research Plasmids and Resources

    Type
    Collection
    ... HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2 HSP90AB1 heat...VIRG, VPAC1, VPCAP1R VIPR2 vasoactive intestinal peptide receptor 2 FLJ16511, VPAC2, VPCAP2R XCL1 chemokine...

  12. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...guanosine, the result is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence...

  14. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are upstream of ...

  15. Plant Plasmids and Resources

    Type
    Collection
    ...Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series...

  16. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...pneumoniae Kanamycin none, need Cas9 plasmid Marraffini pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol yes,...

  18. Control AAV Preps

    Type
    Collection
    ... Cre dependent 1, 2, 5, 8, 9, rg* Roth 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8...

  20. COVID-19 Resources

    Type
    Collection
    .... Joung J, et al. medRxiv 2020.05.04.20091231 AapCas12b plasmid from the Abudayyeh-Gootenberg lab. A one-enzyme...
Showing: 81 - 100 of 103 results