...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

... or protein-protein interactions in deep-tissue organs and whole mammals. Genetically encoded fluorescent...

...Structural Genomics Consortium is a not-for-profit organization that aims to determine the three dimensional...

...Collections A modular set of plasmids from a depositor organized by a common use. Click here to view all collections...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...protein interactions to identify novel receptor-ligand interactions that function in the invasion of the...

...yielded TALENs which function efficiently in: C. elegans (Wood et al., Science 2011) Zebrafish (Sander et...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...available to scientists in academic and industrial organizations. For more information on ordering from a for-profit...

...bar or sort buttons to find plasmids based on: Organism or species (e.g., human, watermelon, Streptomyces...

...detection of low affinity extracellular receptor-ligand interactions. Kerr SJ and Wright GJ. J. Vis. Exp...

...plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal...

...adenovirus plasmids in either mammalian cells or microorganisms, including bacteria and yeast. Two plasmids...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...systems Tag Size (amino acids) Ligand Commercial ligands available Ease of use Tetracysteine...though. Purchasing lots and lots of organic dyes or specialized ligands is more expensive than letting the... synthesizing your own ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry...linked to, say, a chemical dye or other functional ligand, your protein of interest will end up linked to...Just express the fusion protein and add a specific ligand! A self-labeling protein gives you the flexibility...flexibility to choose different colors or ligands for different experiments without having to clone a whole ...structure represents a fluorophore or other useful ligand, gray structure is the protein of interest, and...

...mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact Enhanced signaling through either...

.../Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell . 28(21):2854-2874. PMID: 28814507...

...to one or a subset of organisms. Examples: “organism agnostic”, “model organisms”, “small genome”, “large...require the organism to be specified and are truly organism agnostic. Other tools require an organism to be ...of 2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing for ...only one, or a subset of organisms. In only a few cases the software is organism agnostic. Most tools are...tools will be marked as organism agnostic and have currently available organisms listed in this section...some kind of pre-analysis. At the end of 2014, I began developing software to make sgRNA design accessible...input types can be readily converted.  Examples: “organism”, “sequence”, “identifier”, and “load”. Trends...