...making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element, leading to separation...insert the whole multicistronic cassette into a backbone as a single unit. Although it is recommended to...

...expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories...-White Screening Choosing Your Perfect Plasmid Backbone  ...

...plasmid backbone that was designed specifically for transient expression (find some useful backbones here...

...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

...protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

...an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain no genetic material, ...particles for transient delivery of prime editor ribonucleoprotein complexes in vivo. Nature Biotechnology, 10.1038...

...interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags to your gene,...metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl malate dehydrogenase ...

...plays some role, and mismatches with more hydrogen bonds are more readily ligated than those with few. For...many ligases, G:T mismatches, with two hydrogen bonds and a base-pair size nearly indistinguishable from...

...uses the direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates...//doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (2013) Efficient genome editing in Caenorhabditis...

... show wide scale mobility with movement from the bone marrow and other lymphoid organs to specific tissues... to decipher the journey of lymphocytes from the bone marrow to the thymus where they gain their specificity...

...for the target:probe/primer bonds to be more stable than the hairpin bond.  Alternatively, chemistries...

...domain that promotes the formation of an isopeptide bond between 2 peptide tags, SpyTag and KTag. The group...plasmids for a given species are based on the same backbone to permit expression levels to be directly compared...

...Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 ...Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs ...library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs...genes and containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting...VP64) Set A Human CRISPR inhibition library in backbone pXPR_050 inhibiting 18,901 genes and containing...

...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...LacZ, plus Cre recombinase 60229 : sgRNA cloning backbone with Cre recombinase 60230 : sgRNA targeted to...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described ...control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid ...

...BMP5 bone morphogenetic protein 5 MGC34244 BMP6 bone morphogenetic protein 6 VGR, VGR1 BMP7 bone morphogenetic... MGC34632 BMP1 bone morphogenetic protein 1 FLJ44432, PCOLC, PCP, TLD, pCP-2 BMP10 bone morphogenetic ...protein 10 MGC126783 BMP15 bone morphogenetic protein 15 GDF9B, ODG2, POF4 BMP2 bone morphogenetic protein ...protein 2 BMP2A BMP3 bone morphogenetic protein 3 BMP-3A BMP4 bone morphogenetic protein 4 BMP2B, BMP2B1, MCOPS6...protein 7 OP-1 BMP8A bone morphogenetic protein 8a FLJ14351, FLJ45264 BMP8B bone morphogenetic protein...BMPR1A bone morphogenetic protein receptor, type IA 10q23del, ACVRLK3, ALK3, CD292, SKR5 BMPR1B bone morphogenetic...

...S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm EcoRI none S. pyogenes de Bono pMB60 47941 Worm... cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes Zhang pSpCas9n...none S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes...BsmBI none S. pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian... cut S. pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI... SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian...AAV SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian...

...Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133...localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression ...localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...Mootz HD, Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. ...

...Often, the size of the plasmid insert and vector backbone are known and thus this technique can be quickly...buffer Verifying Total Plasmid Size -OR- Insert and Backbone Size The simplest form of diagnostic digest is...the expected size, or that it is composed of a backbone and insert of expected sizes. This is frequently...in two bands; the 1200bp insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting...know that you have a 1,200bp insert in a 5,000bp backbone, but there are many plasmids out there that, when...very unique pattern that will distinguish one 5kb backbone with a 1.2kb insert from all others. When choosing...orientation the insert is ligated into the vector backbone and therefore the only thing left to do is identify...

... Vincent Pieribone AV-1-36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473...mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-50943 50943-...mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68717 68717-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68719 68719-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68720 68720-...Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-PV2723 98929...pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-PV2521 105551...

...top Empty Backbones The table below highlights plasmids that can be used as empty backbones for your gene...CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background...different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources...expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice. Includes a protocol (Link opens...includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...