...been created in AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells... gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions between the gRNA...disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading and discrimination...endonuclease. Nature , 513 (7519), 569–573. PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson...system. Nature , 493 (7432), 429–432. PMID: 23242138 Bondy-Denomy, J., Garcia, B., Strum, S., Du, M., Rollins... Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa...Marino, N. D., Pinilla-Redondo, R., Csörgő, B., & Bondy-Denomy, J. (2020). Anti-CRISPR protein applications...

...Techniques You may also like... Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme...compatible restriction enzymes, that cut your insert or backbone at only the desired location for your cloning ...putting it under a variety of promoters and into backbones with different selection cassettes). Although ...disadvantage of TOPO® cloning is that very few plasmid backbones are available TOPO® ready, and it is not feasible...overhangs can be efficiently ligated into any plasmid backbone independent of how it was originally designed ...

... for an empty plasmid backbone for your experiment, see Addgene's empty backbone page for more information...of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can...unique and not located elsewhere in the plasmid backbone, which is why they can be used for cloning by ...Antibiotic Resistance Choosing Your Perfect Plasmid Backbone 6 tips for analyzing and troubleshooting sanger...

...23303063 (Link opens in a new window) Gomez JL, Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez...RJ, Richie CT, Harvey BK, Dannals RF, Pomper MG, Bonci A, Michaelides M (2017). Chemogenetics revealed:...Link opens in a new window) Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez MH, Hu X, Galvan...

...the plasmid backbone is meant to be descriptive of the components found within the backbone. For example...

...off-target editing is a major concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the... to generate transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and ...

... Plasmids within a given library have the same backbone, but they express or target different genes. Some...

...equivalent across multiple viruses. The adenoviral backbone pAdEasy-1 is suitable for most purposes. For especially...

... Plasmids within a given library have the same backbone, but they express or target different genes. Some...

...repository, we've chosen primers based on the plasmid backbone and insert. To identify primers that may be useful...