Addgene

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5 Reasons to Use Reddit for Science Communication

Type

Blog Post

...too many to describe here, but I have over 2,000 notes which Evernote even allows me to access on my phone...
CRISPR Activation: A Practical Guide

Type

Blog Post

...you want to activate, you should also be ready to test three or four guides directed towards that gene ...
Twenty Years of Sharing: Addgene's Viral Vector Service

Type

Blog Post

...protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors...
Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid...
Viral Vectors 101: Preparing Pooled Libraries

Type

Blog Post

...amplification. Transformed E. coli are allowed to grow on plates or in liquid culture to amplify the library, followed...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

...doi.org/10.1038/s41565-019-0375-4 Lowder LG, Zhang D, Baltes NJ, Paul JW III, Tang X, Zheng X, Voytas DF, Hsieh...
Plasmids 101: Repressible Promoters

Type

Blog Post

... the strong yeast promoter pTEF). As ethanol accumulates, it binds to the repressor, enabling it to bind...
How to Deposit Your Plasmids with Addgene

Type

Blog Post

...descriptions first before entering the data. This step associates an Addgene ID with your plasmids, and adding ...
Protocol - How to Design Primers

Type

Protocol

...also needs to avoid primer-primer annealing which creates primer dimers and disrupts the amplification process...
Protocol - How to Create a Bacterial Glycerol Stock

Type

Protocol

...2 mL screw top tube or cryovial and gently mix. Notes: Make the 50% glycerol solution by diluting 100%...
Protocol - How to Streak a Plate

Type

Protocol

... Agar Plate You may also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA...
Fluorescence Titering Assay

Type

Protocol

...lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one ...
Lab Safety for Biosafety Levels One and Two

Type

Protocol

... in a final concentration of 10% bleach for 30 minutes before pouring down the drain. Solid BSL-2 waste...
Isolating a Monoclonal Cell Population by Limiting Dilution

Type

Protocol

...PES filter or centrifugation at >500 x g for 5 minutes. Do not use the medium if the cells are overly ...

Showing: 921 - 934 of 934 results

Results per page:

5 Reasons to Use Reddit for Science Communication

CRISPR Activation: A Practical Guide

Twenty Years of Sharing: Addgene's Viral Vector Service

Plasmids 101: Plasmid Incompatibility

Viral Vectors 101: Preparing Pooled Libraries

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Plasmids 101: Repressible Promoters

How to Deposit Your Plasmids with Addgene

Protocol - How to Design Primers

Protocol - How to Create a Bacterial Glycerol Stock

Protocol - How to Streak a Plate

Fluorescence Titering Assay

Lab Safety for Biosafety Levels One and Two

Isolating a Monoclonal Cell Population by Limiting Dilution

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