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  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...edition, we’re going to take a look at a very interesting tool that can be used for creating (excuse the...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...

  3. Design Tips for Prime Editing

    Type
    Blog Post
    ...sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different... edit. If possible, consider following these suggestions: Edit the PAM along with your intended edit....

  4. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...sample protein is retained better, antibodies will stick permanently to a membrane that has been dried, no...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...

  5. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...modifying enzyme) to pair with it can be queried by testing any number in the Level 2 assembly. These pathways...scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, synthetic ...

  6. Hot Plasmids February 2024

    Type
    Blog Post
    ...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...Importantly, AAV1-X1 is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice...

  7. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...mammalian cell expression, and compatible with constitutive or Cre-dependent expression. These sensors have...the calcium sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream...

  10. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ...photostable yellow fluorescent protein to date By Justin Ng High-throughput screening methods, such as fluorescence-activated...

  11. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...impacts the expression level of the transgene. In existing strains of mice, the transgene can be maintained...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    ...six different genes whose protein products have distinct localizations. Figure 2: Split-wrmScarlet...the protein to fluoresce red. H2B-FT-red is long lasting and was shown to have a half life of 84 hours in...

  13. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable to...activation kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option...

  14. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...transfection reagents and keep the cells happy. Harvesting virus Once you have transfected your cells, wait...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...

  18. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways... you use them for! A well-developed biological question and experimental system are absolutely necessary...

  20. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Therefore, FPs are used to study many biological questions. The two most common uses are: 1) to test the ...microscopes the filters are not narrow enough to distinguish between closely related colors. Furthermore, ...
Showing: 881 - 900 of 1033 results