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  1. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...making virus’ or what gag and pol stand for, we demystify all components of viral vectors here! Need Virus...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched...

  2. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...producing the proteins – are not, and many of the existing GCE tools are not very robust. When using GCE,...work. Plus, keep an eye on our blog – we’ll be posting more posts on the various protocols and helping...

  3. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...Image courtesy of National Human Genome Research Institute. Its genome, however, isn’t enough to ensure...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...

  5. Hot Plasmids - February 2022

    Type
    Blog Post
    ...the Genetic Pertubation Platform at the Broad Institute have expanded the genetic screening toolkit by...separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE gels and can ...

  6. Antibodies 101: Isotypes

    Type
    Blog Post
    ...isotype? An isotype determines several key characteristics of an antibody as well as the role it plays... regions result in several unique physical characteristics of these five isotypes, such as the number ...

  11. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...less than an hour, thus a population of cells constitutively expressing a targeted Cas9 should possess indels...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...

  12. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    .... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No...

  14. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...Maybe take a deep breath?" Once you answer the question, you need only to take that step. Repeat that ... to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I've had multiple...

  15. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... while puzzling through your broader research questions and designing future experiments. Being able to...Scientific Method? Imagine your problem is just a question you have about the world. You might do some background...

  16. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...their activity in mammalian systems has been in question. Three different labs have recently published ... the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and PE5. These enhanced...

  18. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...

  20. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...Morgan, our senior engineer? The answer to these questions--which were real, not hypothetical--was clearly...Others in the meeting might also have the same question and asking can help the team identify possible...
Showing: 861 - 880 of 1033 results