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  2. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have..."Enhanced liver gene transfer and evasion of preexisting humoral immunity with exosome-enveloped AAV vectors...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...have been able to study the effects of various stimuli on global translation, but a visual technique to... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing...

  5. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...either fission or budding, which is the primary distinction between two of the most frequently used yeast...highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is because...

  6. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...misinformation about sexual and reproductive health, as the stigma around sexual and reproductive health issues creates...never forgot about all the anxious, whispered questions from friends and classmates back in high school...

  7. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress. ...one convenient resource. I can safely say that I still have the single and triple abbreviations of the ...

  8. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...spectra differ sufficiently for them to be imaged in distinct fluorescent channels on the microscope. With the... to choose fluorescent proteins that can be distinguished from one another and that can be imaged with...

  9. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...luciferase-containing plasmid is most often used to investigate the effect of regulatory elements, such as promoters...Pathways Known cellular signaling pathways can be investigated in a high-throughput screen using luciferase...

  11. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...advice for using Twitter, this post provides suggestions for using LinkedIn and Facebook. For each of ...the three social media platforms, there’s some statistics on what percent of scientists and engineers regularly...

  13. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...

  14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...to identify cells that took up the vector and distinguish between plasmids that contain the gRNA (white...Walkowski.   Restriction digest Another way to distinguish between colonies containing plasmids with inserts...

  15. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...

  17. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ... from their native conditions of ~0°C, which drastically affects their stable folding and efficient functioning...beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team has continuously...

  19. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in one direction...plasmids available from Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them...
Showing: 841 - 860 of 1033 results