We narrowed to 873 results for: lic

1. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

2. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall...

3. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments...

4. CRISPR 101: Making a Knock-In Cell Line

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   ...nutrients in their media, they will not undergo DNA replication, so make sure you are keeping an eye on those...

5. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   ...therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section. In order to denote...

6. The Importance of a Fun Workplace: Company Culture at Addgene

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   Blog Post

   ...be an obvious one, but don’t make things too complicated for yourself (especially if you are the sole ...

7. Plasmids 101: Protein tags

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   ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

8. Negotiating Work and Life: How to Find the Joy

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   ...can make a decent meal, fold their laundry, take public transportation, call a cab and remember their own...

9. CRISPR 101: Any Base Transversion Editors

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   ...researchers can now select the best tool for their application from among many options.  Conclusion With these...

10. Twenty Years of Sharing: Expanding Accessibility

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    ...Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design Your gRNA for...

11. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...approach and involves reading primary literature, soliciting information from requestors and depositors, and...

12. Antibodies 101: The Four ELISAs and When to Use Them

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    ...). Fluorescent tags may also be used, but for simplicity we will discuss the more common enzyme-based ...

13. How to Be an Excellent Trainee

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    ...tell a trainee is understanding and things are ’clicking’ for them. It’s often easiest to train individuals...

14. To Codon Optimize or Not: That is the Question

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    ...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org...

15. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...such as those that affected gene expression, mRNA splicing, or protein coding regions. In total, they tested...

16. Designing Your Chalk Talk for the Academic Job Interview

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    .... Given this large sum, one might expect that applicants would work for months to hone a chalk talk. Yet...

17. Plasmids 101: Codon usage bias

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    ...understood, but some possible reasons include: Metabolic pressures - it takes cellular resources to produce...

18. Neuronal labeling with Spaghetti Monster

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    ...opposition to the teaching of intelligent design in public schools and was started in 2005 in Kansas by Bobby...

19. Transferable Skills Guide: External Collaborations

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    ...skill is a hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual...

20. Viral Vectors 101: Pseudotyping

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    ...The glycoprotein will be incorporated into the replicating rabies virus envelope, travel retrogradely, and...