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TypeBlog Post... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...
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Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...Each half of the base editor is fused to a fast-splicing split intein and co-infected into the cell. Intein...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post... this stage and that can be used to simulate in silico cloning. This will definitely help in understanding...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...modular suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow ...Central PMCID: PMC5963681. Meyer, Richard. "Replication and conjugative mobilization of broad host-range... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post... With the addition of blue light, the terminal helices of the LOV2 domain unfold, disrupting the protein...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...Brazil. Through an expansion of our export license application service, we’re pleased to announce that we... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF.... Sensitivity In ex vivo studies on mouse brain slices, nLightG and GRABNE were more sensitive than nLightR... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...and sorting. Reference images can be viewed by clicking on the links in the "Agree" and "Disagree" columns... repository for reagents, protocols, panels, publications, software, and datasets. It’s also a great place... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670... -
Build Your CRISPR Vocabulary
TypeBlog Post...Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...contain splice acceptors. They require insertion into an intron of a gene, where they can be spliced into... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...is resistant to specific compounds, links to publication references, and cross-references with cell line...manually going through the pdfs of the 26,000 publications cited. If the information seems incomplete or... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could have a major...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene. CRISPR-Act3.0, ... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...takes minutes. As a result, it is not possible to elicit an immediate response or track the exact time of...beneficial for use in therapeutic or behavioral applications where drawn out effects are required. Targeted... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post... mutations in single stranded DNA undergoing replication. DNase on the other hand can digest and degrade...directly use your DNA preparation in any downstream application involving enzymatic reactions, a valid alternative... -
Fostering communication in remote collaborations
TypeBlog Post...meetings as opposed to phone calls most closely replicates how you’d interact with team members in person...access data and drafts of manuscripts and grant applications in real-time so they can provide input at the... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post... a Cas9 nickase and two gRNAs. For nickase applications, a common question is: how should the gRNAs ...information and a link to the archived webinar, click here. We would like to thank IDT scientists Mollie... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...promoters, selectable markers, tags, origins of replication, and fluorescent proteins that are already included...the plasmid sequence with one or more enzymes. Click here to learn how to run an agarose gel We’...