Which Fluorescent Protein Should I Use?
Type
Blog Post
.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues” Physiol...
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New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...bring in things to be physically interacted with. Public speaking is a common fear. Can be discouraging ... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...nutrients in their media, they will not undergo DNA replication, so make sure you are keeping an eye on those... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section. In order to denote... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...be an obvious one, but don’t make things too complicated for yourself (especially if you are the sole ... -
Plasmids 101: Protein tags
TypeBlog Post...deciding on which one to use will depend on your application (see table below). The antibodies available for... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...can make a decent meal, fold their laundry, take public transportation, call a cab and remember their own... -
Plasmids 101: Codon usage bias
TypeBlog Post...understood, but some possible reasons include: Metabolic pressures - it takes cellular resources to produce... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...opposition to the teaching of intelligent design in public schools and was started in 2005 in Kansas by Bobby... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...skill is a hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...such as those that affected gene expression, mRNA splicing, or protein coding regions. In total, they tested... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post.... Given this large sum, one might expect that applicants would work for months to hone a chalk talk. Yet... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design Your gRNA for... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...approach and involves reading primary literature, soliciting information from requestors and depositors, and... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...). Fluorescent tags may also be used, but for simplicity we will discuss the more common enzyme-based ...