We narrowed to 870 results for: lic

1. CRISPR 101: Making a Knock-In Cell Line

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   ...nutrients in their media, they will not undergo DNA replication, so make sure you are keeping an eye on those...

2. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   ...therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section. In order to denote...

3. The Importance of a Fun Workplace: Company Culture at Addgene

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   ...be an obvious one, but don’t make things too complicated for yourself (especially if you are the sole ...

4. Plasmids 101: Protein tags

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   ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

5. Negotiating Work and Life: How to Find the Joy

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   ...can make a decent meal, fold their laundry, take public transportation, call a cab and remember their own...

6. CRISPR 101: Any Base Transversion Editors

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   ...researchers can now select the best tool for their application from among many options.  Conclusion With these...

7. Twenty Years of Sharing: Expanding Accessibility

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   ...Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design Your gRNA for...

8. Twenty Years of Sharing: Addgene's Viral Vector Service

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   ...approach and involves reading primary literature, soliciting information from requestors and depositors, and...

9. Antibodies 101: The Four ELISAs and When to Use Them

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   ...). Fluorescent tags may also be used, but for simplicity we will discuss the more common enzyme-based ...

10. Viral Vectors 101: Pseudotyping

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    ...The glycoprotein will be incorporated into the replicating rabies virus envelope, travel retrogradely, and...

11. To Codon Optimize or Not: That is the Question

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    ...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org...

12. How to Be an Excellent Trainee

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    ...tell a trainee is understanding and things are ’clicking’ for them. It’s often easiest to train individuals...

13. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...such as those that affected gene expression, mRNA splicing, or protein coding regions. In total, they tested...

14. Designing Your Chalk Talk for the Academic Job Interview

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    .... Given this large sum, one might expect that applicants would work for months to hone a chalk talk. Yet...

15. Transferable Skills Guide: External Collaborations

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    ...skill is a hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual...

16. Plasmids 101: Codon usage bias

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    ...understood, but some possible reasons include: Metabolic pressures - it takes cellular resources to produce...

17. Neuronal labeling with Spaghetti Monster

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    ...opposition to the teaching of intelligent design in public schools and was started in 2005 in Kansas by Bobby...

18. Tips for CRISPR Gene Editing in Mice

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    ...et al., 2014). The plasmid is sturdier than the delicate RNA and therefore there has less chance of contamination...

19. Tips for Using FRET in Your Experiments

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    ...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

20. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...is an umbrella term for a number of different applications that involve different types of small RNA molecules...