Skip to main content
Addgene
Showing: 821 - 840 of 1033 results

  4. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...plasmid in addition to its own chromosomal DNA, costing additional resources to maintain the plasmid. Adding...cell division (bacteriostatic); however, the distinction between the two categories may be a bit of a ...

  5. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...of red to green fluorescence allows for clear distinction between vacuolar/lysosomal and cytoplasmic Rosella...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence...

  11. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy to determine...Backstrom, Ph.D is a biochemist in the Vanderbilt Eye Institute and is particularly interested in cell signaling...

  12. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...But what makes software good? Fortunately, that question has been thoroughly answered by experts in human-computer...engaging with users of the software. We welcome suggestions about common features from anyone who uses SnapGene...

  13. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...because the secondary antibody is not able to distinguish between the primary antibody and endogenous IgGs...your samples, but know that all is not lost if you still see non-specific staining! Hopefully, these solutions...

  14. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ... viral vectors, which are used in the lab, are distinct from viruses — we’ll get to that in a bit! Viral...bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast to ...

  15. What the HEK?

    Type
    Blog Post
    ...the fetal tissue which HEKs were isolated from is still a mystery, but it’s currently assumed that the cells...native channels.  No matter what your research question, HEK-293 is known for reproducible results. It...

  20. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...researchers. For many years I struggled with the questions of “Will I leave academia?” and "What type of ...the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled. ...
Showing: 821 - 840 of 1033 results