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  1. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... FPs since its emission wavelength (612 nm) is distinct from other commonly used FPs, and GFP does not...localization that have been encountered by some investigators when using forms of mEos." The ability to repurpose...

  2. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ..., designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence for translation ...integral-membrane protein constructs) protein tag. MISTIC drags tagged proteins into the membrane and may...

  4. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...? This final post in the PI Interview Series investigates how these three PIs reward their team and manage...during the child-rearing years, learning “lots of interesting things from children, their classmates, and parents...

  5. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...frequently used pages in your notebooks and go wild with sticky notes and labels. Color code your Post-its and ...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you...

  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...localization of individual proteins, but also investigate protein-protein interactions via FRET. Sidae ...partial gene deletion, overexpression (by promoter substitution), and tagging at either the N- or C-terminus...

  11. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...two gRNAs.  For nickase applications, a common question is: how should the gRNAs be oriented in comparison...insertions. These profiles are likely due to the distinct overhang patterns generated by the nickases; D10A...

  13. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...

  14. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...for new ways to serve the research community. Questions? Feel free to reach out to us on Twitter, Facebook... Ishrat, Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, ...

  16. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...process known as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t...

  17. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...sometimes even genetic pathways that are worth investigating for drug development. Due to the difficulty ... to determine if a patient would respond to an existing therapy. In theory, CRISPR could even be used ...

  18. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... but boy does it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is ...appears between each spacer in the CRISPR array, distinguishing the foreign sequences and keeping the array...

  20. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ... my kids like any other youngster. His parents, Kristi and Rico, had to keep him from running too much...the fight against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many ...
Showing: 801 - 820 of 1033 results