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  1. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...researcher decides on a new project and begins by trying to reproduce someone else’s result. Then, they ...that they thought would be moving forward instead trying just to get going. It’s like being stuck in jail...
  2. 2022 Depositor Week

    Type
    Blog Post
    ... enough - but this week we’re certainly going to try!      Here’s our Depositor Week schedule:  Monday...
  3. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...from Ma et al., 2018 with permission.   Why try CRISPR-Sirius? With all the tools out there to visualize...visualize genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References...
  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ....  We hope you will give our new search engine a try to find reagents and educational material. Let us...us know what works for you and what doesn’t. Try out the new Addgene search engine! Additional resources...
  5. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...Relationships for Success”. Choose 1-2 tactics to try. One of my favorites is making the pledge to have... being an educated mentee. Other opportunities: Try virtual mentoring at Mentornet.net. This is an online...seeing a lot of success at universities around the country with groups who are piloting this. Watch for my...
  6. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...occasional errors. That can be advantageous if you are trying to knock out a gene. One can also exploit homology-directed...induced pluripotent stem (iPS) cells from patients and try to compare those lines, they may all have the mutations...
  7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...RNA structure and the site in the genome you are trying to target play a large role in Cas9 activity and... CRISPR mutant of interest, give these methods a try.  References Højland Knudsen C, Ásgrímsdóttir...homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722...
  8. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...that blue and violet light can penetrate. How can I try using photoswitchable kinases in my experiments? ...into loops of a protein of interest. If you plan to try this, it’s important to check that the function of...
  9. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...send an unpersonalized, generic invitation. Don’t try to connect from the LinkedIn phone app either as ...suit, but this is not Facebook so no pets or kids. Try looking a bit over your shoulder for the picture....
  10. To Each HIS Own

    Type
    Blog Post
    ...during protein folding, in which case you should try tagging the opposite end.  If neither placement works...protein of interest is expressed at a very low level, try a longer histidine tag to increase capture. Longer...
  11. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...nitrocellulose, you may decide you have nothing to lose by trying to strip and reprobe. In this case, we recommend...procedure, perhaps using longer incubation times, or try the stringent solution if you started with the mild...
  12. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...gondii genes.  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene...activation, repression, and barcoding.  3) Are you trying to target every gene in the genome, or a specific...
  13. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ... you. When embarking on a new literature search, try to find a recent review article to give you an overview...you are looking for a particular protocol or are trying to replicate an experiment, the methods section... you to our guest blogger! Johnna Roose is a biochemistry instructor in the Department of Biological Sciences...photosynthesis and writes about autotrophs and biochemistry on her blog. Follow her on Twitter @JLRoose....
  14. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...gloves can sometimes be annoying. To make it easier, try this quick trick. Just flip, and press! Lab tip...
  15. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...not doing yourself or your science any favors by trying to go it alone. The help of fellow lab members .... If I invite someone to be on a project team, I try to make it very clear why that person is there. If...thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble to attack this problem...
  16. Finding Your Perfect Job After University

    Type
    Blog Post
    ... get a lot of office politics (many individuals trying to divert more resources towards their projects...modern genetics from the perspective of somebody trying to get hands-on lab experience is that the technology... may seem quite unusual but it has allowed me to try out working in many different sectors and to discover...applied for a role in Industry. I wanted to get "a real job" and roles in Industry do tend to pay better... (GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however... a little different. Industry and academic jobs do exist, but working in industry just wasn't for me, ...months experience working in a developing tropical country. This is why I set up a placement working in a ...
Showing: 61 - 80 of 520 results