...the same replicon are incompatible because they compete for the same replication control machinery within... concept is a little more complex than simply competing for replication control factors and tends to be...plasmids with the same centromere-like binding region compete for the same CBPs and NTPase to correctly partition...blocking replication of another and one plasmid outcompeting the other plasmid for replication or partitioning...machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid...

...want to use electro-competent cells instead of the more common chemically-competent cells. You should perform...Follow the manufacturer’s instructions for your competent cells. For most standard cloning, you can transform...transform 1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using much ...getting colonies, you might want to use higher competency cells. Additionally, if your final product is...

... appreciated the creative uses of old posters, pipette tip boxes, and more (stay tuned for a few blog ...sustainability in the lab!). The @ladiesinlabcoats used a pipette tip box and microcentrifuge tubes to make Eppi ...labchristmas #cute #reindeer #DeckTheLab #eppendorf #themuppets #scifun #funny #ChristmasScene #womenwhoscience...all who participated in the 2018 #DeckTheLab competition. The four winners will receive a holiday care...

...forming a vector that could create replication competent virus. Even though this system was efficient at...potential risk for the generation of replication competent lentiviruses (RCL) especially if you are working...positive cells could make the virus replication competent. The 3rd generation of lentiviral vectors was...8 kb) Potential for generation of replication-competent lentivirus (RCL) Efficient gene transfer Potential...Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human tropism can still infect...

...lab. Iron Chef-style competitions Every few months, we’ll have a cooking competition featuring a selected..., which is either an indication of the stiff competition or says something about my cooking.   This is... to organize. We’ll dedicate a post to these competitions in the future, and hopefully include some of...

...great approach to fully understand and acquire competitive skill sets in a myriad of professions is to engage...Mentorship Leadership Science writing competitions (e.g. Access to Understanding)   Writing...Communication Networking Publicity Business competitions   Leadership Management Marketing ...opportunity to tailor their career paths for competitive positions both within and outside academia. Use...

... into your data. Try different types of pipettes and pipetting methods to find what’s most consistent ...plate in a way that will tell you if you have pipetting errors on the edges of the plate. Compare the ...multiple configurations can help you identify pipetting or evaporation errors. And if time allows, you...BioRender.   Passive Reference Dyes Whatever your pipetting method, some variation will occur. A passive reference...

...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round data.... Bobae An will host a Q&A session after the competition, where you can get all your burning AAV questions...

...scientists washed and reused materials such as glass pipettes or inoculation loops. While single-use is now ...a single use. So how about washing and reusing pipette tips in a high-throughput and efficient way? Grenova... devices that are compatible with a variety of pipette tips. This is part of a cultural change in how ...

...methylation competent E. coli for this procedure to work properly. Directly transform chemically competent cells...are then transformed directly into chemically competent E. coli and transformants selected with the appropriate...

... Gram-negative and Gram-positive bacteria Jason Peters, Oren Rosenberg, and Carol Gross used two different...CRISPRi to a much wider swath of bacterial species (Peters et al., 2019). The systems differ on how exactly...10: . https://doi.org/10.1038/s41467-019-11479-0 Peters JM, Koo B-M, Patino R, Heussler GE, Hearne CC, ...Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA,...

...30678704. Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein Sparse...Sparse Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just...heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively remove a stop...

...relies on two distinct Cas9 variants—nuclease-competent or nuclease-null—in order to effect different ... to Cas9. For instance, when native (nuclease-competent) Cas9 interacts with a ≤15nt gRNA that contains...delivered into systems that already express nuclease-competent Cas9, obviating the need to generate new cell ...

...as a device fits on the deck, and it’s easy to pipette samples in and out of it, you can set up a procedure...using electronic multichannel and single channel pipettes. These can speed up any project in which you need... to do a lot of aliquoting. Simply program the pipette with how much total sample you need and the volume...

... samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received...ensure that nothing is contaminated. We use filtered pipet tips and prepare fresh media with antibiotics at...Each sample that is transformed is plated onto a petri dish plate that is also barcoded. The barcodes on...

...Professional Development Strategy to increase your competencies and skills in the grants profession. Your professional... and the candidate’s knowledge of the 8 core competencies through a multiple choice exam.  The bottom ...grant professionals and to build your skills and competencies. Becoming a grant professional is rewarding ...

...mapped to a SNP would be ignored. Working with Lia Petronio and Katherine Huang from Broad’s Pattern team,...between biology and ancestry group as a means of perpetuating racial bias. It is easy, even unintentionally...Noorbakhsh, Isabella Boyle, Joshua Dempster, Lia Petronio, Katherine Huang, Alham Saadat, Thomas Green, ...

...Have a dedicated bench with a dedicated set of pipettes for ddPCR set-up. Prepare the master mix in a ...the bench and all consumables with 10% bleach.  Pipette slowly If you are using a manual droplet generator...automated droplet generator, you should avoid pipetting too quickly when making your virus dilutions. ...

...decreasing the probability of creating replication-competent lentivirus (RCL) in your target cells.  Q4: How...targeted. Q6: Are transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer...are: 1) the potential generation of replication competent virus [usually HIV-1]; and 2) the potential for...

...just one or two recombination events, homemade competent cells of 107 CFU/μg are perfectly fine, yet performing...performing multiple modifications will require ultracompetent (109 CFU/μg) cells. Primer design Using specific...(1991): 62-66. PubMed PMID: 2003926. 2. Bubeck, Peter, Monica Winkler, and Wilfried Bautsch. "Rapid cloning...