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We narrowed to 301 results for: pET

Showing: 61 - 80 of 301 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...forming a vector that could create replication competent virus. Even though this system was efficient at...potential risk for the generation of replication competent lentiviruses (RCL) especially if you are working...positive cells could make the virus replication competent. The 3rd generation of lentiviral vectors was...8 kb) Potential for generation of replication-competent lentivirus (RCL) Efficient gene transfer Potential...Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human tropism can still infect...

  3. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...lab. Iron Chef-style competitions Every few months, we’ll have a cooking competition featuring a selected..., which is either an indication of the stiff competition or says something about my cooking.   This is... to organize. We’ll dedicate a post to these competitions in the future, and hopefully include some of...

  4. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...great approach to fully understand and acquire competitive skill sets in a myriad of professions is to engage...Mentorship Leadership Science writing competitions (e.g. Access to Understanding)   Writing...Communication Networking Publicity Business competitions   Leadership Management Marketing ...opportunity to tailor their career paths for competitive positions both within and outside academia. Use...

  5. Deep Dive: qPCR

    Type
    Blog Post
    ... into your data. Try different types of pipettes and pipetting methods to find what’s most consistent ...plate in a way that will tell you if you have pipetting errors on the edges of the plate. Compare the ...multiple configurations can help you identify pipetting or evaporation errors. And if time allows, you...BioRender.   Passive Reference Dyes Whatever your pipetting method, some variation will occur. A passive reference...

  6. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    ...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round data.... Bobae An will host a Q&A session after the competition, where you can get all your burning AAV questions...

  7. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...a relative term — even armed with the highest-competency cells you will still struggle to get transformants... or if you get a little overzealous with your pipetting. Another is the possibility of carrying over contaminants...practices for the lab. Plastic consumables (including pipette tips, plates, and yes those purification columns...buffer bottles Fewer plastic consumables Fewer pipette tips needed in workflow Reduced waste from ...

  8. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...scientists washed and reused materials such as glass pipettes or inoculation loops. While single-use is now ...a single use. So how about washing and reusing pipette tips in a high-throughput and efficient way? Grenova... devices that are compatible with a variety of pipette tips. This is part of a cultural change in how ...

  9. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... Gram-negative and Gram-positive bacteria Jason Peters, Oren Rosenberg, and Carol Gross used two different...CRISPRi to a much wider swath of bacterial species (Peters et al., 2019). The systems differ on how exactly...10: . https://doi.org/10.1038/s41467-019-11479-0 Peters JM, Koo B-M, Patino R, Heussler GE, Hearne CC, ...Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA,...

  10. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ... to help them spread through bacterial genomes (Peters et al., 2017). Type I CAST systems rely on the ...CAST activity allowed certain mutated phages to outcompete their brethren, and the researchers isolated ...–225. https://doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova, K. S., Shmakov, S., & Koonin,...

  11. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...methylation competent E. coli for this procedure to work properly. Directly transform chemically competent cells...are then transformed directly into chemically competent E. coli and transformants selected with the appropriate...

  13. Lab Automation at Addgene

    Type
    Blog Post
    ...as a device fits on the deck, and it’s easy to pipette samples in and out of it, you can set up a procedure...using electronic multichannel and single channel pipettes. These can speed up any project in which you need... to do a lot of aliquoting. Simply program the pipette with how much total sample you need and the volume...

  14. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...that allow their host organisms to thrive in competitive environments. The genes encoding these functions...triumph over your neighbors is to eliminate the competition, and Penicillium isn’t the only organism that... are also less aggressive ways to survive in competitive environments — like by living where no one else...

  15. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...mapped to a SNP would be ignored. Working with Lia Petronio and Katherine Huang from Broad’s Pattern team,...between biology and ancestry group as a means of perpetuating racial bias. It is easy, even unintentionally...Noorbakhsh, Isabella Boyle, Joshua Dempster, Lia Petronio, Katherine Huang, Alham Saadat, Thomas Green, ...

  16. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...Have a dedicated bench with a dedicated set of pipettes for ddPCR set-up. Prepare the master mix in a ...the bench and all consumables with 10% bleach.  Pipette slowly If you are using a manual droplet generator...automated droplet generator, you should avoid pipetting too quickly when making your virus dilutions. ...

  17. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...just one or two recombination events, homemade competent cells of 107 CFU/μg are perfectly fine, yet performing...performing multiple modifications will require ultracompetent (109 CFU/μg) cells. Primer design Using specific...(1991): 62-66. PubMed PMID: 2003926. 2. Bubeck, Peter, Monica Winkler, and Wilfried Bautsch. "Rapid cloning...

  18. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ... samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received...ensure that nothing is contaminated. We use filtered pipet tips and prepare fresh media with antibiotics at...Each sample that is transformed is plated onto a petri dish plate that is also barcoded. The barcodes on...

  19. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...Professional Development Strategy to increase your competencies and skills in the grants profession. Your professional... and the candidate’s knowledge of the 8 core competencies through a multiple choice exam.  The bottom ...grant professionals and to build your skills and competencies. Becoming a grant professional is rewarding ...

  20. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...decreasing the probability of creating replication-competent lentivirus (RCL) in your target cells.  Q4: How...targeted. Q6: Are transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer...are: 1) the potential generation of replication competent virus [usually HIV-1]; and 2) the potential for...
Showing: 61 - 80 of 301 results