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We narrowed to 125 results for: multiplex

Showing: 61 - 80 of 125 results

  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...

  2. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...vectors. In 2017, Zetsche et al. developed a Cpf1 multiplexing approach using a single crRNA array to express...publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing by CRISPR–Cpf1 Using a Single crRNA...

  4. Synthetic Biology - Overview

    Type
    Collection
    ...Propionate Induction Lim Lab RNA Feedback Lu Lab Multiplexed/Programmable Gene Networks Mascher Lab Bacillus...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ... experiments for multiplexed targeting with Zhang lab plasmids? A6: For multiplexing CRISPR to target ...Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/CAS systems. Science...

  7. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable nucleic acid detection platform ...(C2c2) Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources on Addgene.org...

  8. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

  9. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...benefits of this technique. It is even possible to multiplex ultrasound dyes because these dyes “collapse” ...

  10. Microbiology Resources

    Type
    Collection
    ... in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression in E. coli - Koffas...

  13. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv (2019): 335547...library-specific identifiers act like a timestamp and help demultiplex the scRNA-seq data and build multi-generational...

  17. Antibody Guide

    Type
    Guide
    ... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...targets, and is described in more detail in the Multiplex Assays section below. The following table describes...distinguish between the primary antibodies in multiplex assays. This is done by using different isotypes...means the majority of IF assays are considered multiplex assays, even if they only have one protein of ...additional markers to distinguish cell types. Multiplex assays can range from two antibodies to upwards...currently are in the mid-forties. When designing a multiplex assay, selection of the fluorophores used is important...

  18. CRISPR Guide

    Type
    Guide
    ...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments involve...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...all desired genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes ...with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target anywhere ...achieved targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate...enzymes, such as Cas12a or Cas13 , can improve multiplexing efficiency and are discussed in more detail ...

  20. Hot Plasmids - January 2023

    Type
    Blog Post
    ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:...
Showing: 61 - 80 of 125 results