...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...MarathonRT: A highly processive reverse transcriptase Article contributed by Will Arnold  Listen to the MarathonRT...monitor retrograde transport at the cell surface Article contributed by Marcy Patrick  Listen to the nanobody...proteins occupying a specific genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex...proteins occupying a specific genomic locus: C-BERST Article contributed by Klaus Wanisch  Listen to the C-...

...of virus, not the individual viral particles. A single viral particle is called a virion. Finally, viral...they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not just ...many After a virion infects a cell, the single particle creates many copies of itself, sometimes up to...eliminate their replicative ability. The remaining particle, which retains the protein coat (and envelope)...genetic payload to a cell but cannot create new particles. As a bonus, this increases the carrying capacity...

...will ensure the virus gets produced, and viral particles are packaged, when the vector is expressed. Once...expressed within the production cell line, viral particles will be produced and packaged, resulting in functional...functional virions. These viral particles can infect other cells, but since the majority of the viral ...be collected for the transduction step. Viral particles are secreted into the cellular media, making viral...like to infect with the virus. Then the viral particles do the rest! Downstream expression or integration...1: Transduction to expression process. A viral particle enters a host cell, enters the host nucleus, is...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...   A retroviral mouse CRISPR knockout library Article contributed by Alycia Bittner CRISPR pooled libraries...reporter for measuring ApoB bound lipoproteins Article contributed by Leah Schwiesow A leading cause of...transcription for CRISPR activation in bacteria Article contributed by Will Arnold Baojun Wang’s lab recently...Genetically encoded voltage indicators for use in worms Article contributed by Brook Pyhtila Genetically encoded...

...   Articles with and without plasmids at Addgene To find out whether scientific articles associated...bibliographic information and uses its vast network of articles to track the total number of citations the paper... of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations So what...estimates based on the journal that published the articles.  That is, we re-estimate the citations to the...weights. Table 1: Journal Weighting Example Article Number (A) Year Published (B) Number of Citations...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...efficient RNAi construct for C. elegans gene knockdown Article contributed by Alyssa Cecchetelli  Listen to the... proximity labeling with TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID.... Changing the way we detect viral outbreaks Article contributed by Izzy Mueller  Listen to the viral...collection reveals novel peptide ligand motifs Article contributed by Michael Lemieux  Listen to the SH3...

...to as hosts) via viral particles, the functional infection unit. Each particle has a viral envelope, which...the target cell’s membrane, allowing the viral particle access into the cell. The viral envelope and its...cargo – its own genome.   Fig. 1: Viral particle infection into a host cell. Image courtesy of ... measures, like replication incompetent viral particles, are required. We will be covering these safety...localization signals which can traffic their viral particles inside the nucleus, without the need for envelope...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...here.        AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great ...fluorescent genetically encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded...: 31685893. A MoClo toolkit for cyanobacteria Article contributed by Andrew Hempstead The Modular Cloning...optogenetic tool for precision in cell lineage tracing Article contributed by Erin Sanders CreLite, designed by...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-...using the fluorescent voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage...studies easier with a 2in1 vector cloning system Article contributed by Michelle Cronin  Listen to the 2in1...tools for photoswitchable proteins cPYP and AsLOV2 Article contributed by Angela Abitua  Listen to the photoswitchable...

...Didier Trono's lab webpage explains how lentiviral particles are produced by co-transfecting three essential...envelope glycoprotein will be expressed on virus particles? A5: This depends on the envelope plasmid you ...common due to its broad host range, and increased particle stability; however, the protein is cytotoxic precluding...target cells, but cannot produce any new viral particles after the initial infection. SIN is shorthand ...all the components necessary to produce viral particles. The components are divided as follows: Transfer...Meghan Rego contributed to the writing of this article. Additional Resources: Didier Trono Lab: Lentivectors...

...transfected into target cells which produce lentiviral particles.   Why pseudotype? Lentiviruses and retroviruses...4 lab. For more details, check out this review article published in Reviews in Medical Virology (Li et... proteins have been pseudotyped on lentiviral particles. Rabies glycoprotein The rabies glycoprotein targets...suit your needs. Check out some of these review articles to find tables and information on different viral...Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization...Jr (2000) VSV-G Pseudotyped Lentiviral Vector Particles Produced in Human Cells Are Inactivated by Human...

...lab used SNAP-tag to decorate synthetic viral particles with BG-linked antibodies to drive cell type-specific...intracellular targets. Nature Communications, 7(1), Article 1. https://doi.org/10.1038/ncomms10778 Erdmann,...molecules in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765 Lavis, L. (2016...A flexible chemical tag. Nature Methods, 2(4), Article 4. https://doi.org/10.1038/nmeth749 Strebinger,...envelope design. Nature Communications, 14(1), Article 1. https://doi.org/10.1038/s41467-023-40788-8 Ulrichs...capping protein. Nature Communications, 14(1), Article 1. https://doi.org/10.1038/s41467-023-39655-3 Wilhelm...

... Chromatin research articles with plasmids available from Addgene Many articles reporting chromatin-related...plasmids from hundreds of articles in our collection; the following articles are just a small cross-section...jumping off point. The following lists of PIs, articles, genes, and blog posts are not comprehensive, ...

...very confusing for scientists writing research articles. What should you take into account when writing...funders of your research may stipulate that your article must be published in an open access journal or ...whether they agree with the conclusions of the article. These initiatives are another way in which journals...report are permanently attached to the published article. In addition, authors and readers are able to comment..., the authors can publish a new version of the article, while the first version and the referee reports...

...from formal mechanisms like publishing a research article, presenting a conference poster or talk, or submitting...avoid many of these issues, by sharing their work publicly and immediately once the manuscript is drafted...somewhat meaningless) practice of listing “Submitted” articles in their CV or grant applications. A number of...source or were deposited when the now-published article was a preprint. Depositing plasmids that were used... as sharing those plasmids alongside a journal article, but it makes the research materials available ...

... viral shRNA expression vector and the vector particles are delivered into the brain region of interest...vectors are used for intracerebral injections. AAV particles are smaller and may be preferable if wider diffusion...via injection into the liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue... titer: The concentration of infectious viral particles must be high enough to maximize possible outcomes...injection speed has to be slow to allow the viral particles to diffuse away from the injection site, and also...

...during mitosis, support transport of organelles and vesicles, and more. Although several strategies are available... demonstrated its use to probe cell mechanics, vesicle transport, and cell division, but given the long...Aljiboury et al., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but with dozens...Figure 3: Optogenetic inhibition of Rab protein vesicle trafficking via CRY2-CIB1 clustering. Image adapted... the plasma membrane or lysosomes (the earlier article also provided CIB constructs for targeting Golgi...

...repository through our hot plasmids articles. These brief articles provide a synopsis of a plasmid or ...functions and applications. We hope that these articles make it easier for you to find and use the plasmids...glia, have been successfully targeted via viral particles, an effective approach to target specific neuronal...HF-BE3 protein for delivery in ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse...localize TRF2 to telomeres. The plasmids used in this article have been deposited with Addgene and include MYC...

...10 Hot Plasmid articles with each of our newsletters. You can find all of these articles on the Hot Plasmids...access these snippets here or from their respective articles on the Hot Plasmid webpage. We hope you enjoy ...Our Newsletter Check Out All of Our Hot Plasmids Articles Browse the Repository  ...

... lab experiences, findings, and experiments as articles written for general audiences. So far, we are ...feedbacks from our non-scientist readers about how our articles have helped them understand different topics of...launch an e-magazine this year which would feature articles, posts, and sci-art that would ultimately give...Addictive Brain. A contribution can either be an article, a short post, a video, or a piece of sci-art.  ...