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Showing: 61 - 80 of 625 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The..., and a pair of annealed oligos can be cloned scarlessly into the vector before the sgRNA scaffold. The...top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ...mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids as well as a backbone...

  2. Resolute Plasmid Collection

    Type
    Collection
    ...reagents to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection...Initiative Collection NeuroMab Collection Solute carrier proteins (SLCs) are the largest family of transporters...experiments. These libraries target the solute carrier family of proteins. Library ID PI Description Human...

  3. Cre-lox system

    Type
    Collection
    ...31132 pCSCre Cre CMV Xenopus Ryffel 31309 pCARCre Cre cardiac actin Xenopus Ryffel 32144 pJFRC170-3XUAS-IVS-Cre...inducible - rrTA expression driven by mouse Nkx cardiac enhancer and promoter fragment minimal TetO-CMV...69916 pAAV.cTNT.iCre iCre and tdtomato Chicken cardiac troponin T AAV Pu 70120 pEMS2159 iCre with MCS ...Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre Cre Chicken cardiac troponin T AAV Pu 134308 pCAG-Synaptophysin-TdTomato-IRES2...

  4. Lentiviral Prep Service

    Type
    Collection
    ...Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library. Version...Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding library. Version...Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding library. Version...

  5. Brain Initiative Collection

    Type
    Collection
    ...pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE Expresses optimized PdCO in frame with mScarlet under control of...

  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... the sensor's behavior in the target system. Use care when applying a biosensor in a novel context or ...proteins enable metabolic assays at the point of care. Science. 2018 Sep 14;361(6407):1122-1126. Kai Johnsson...-regulatory role of the mitochondrial pyruvate carrier MPC. eLife. 2020 Mar 6;9. pii: 53917. Luis Felipe...sensor modules Grashoff Lab Tension Sensor Plasmids Carsten Grashoff Tension tCRMod tension sensors Tunable... voltage sensor to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage...

  8. Antibody Plasmid Collection

    Type
    Collection
    ...Companion to Methods in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector...J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation...

  9. Plant Plasmids and Resources

    Type
    Collection
    ...species. In addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections...microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system based on...

  10. Zebrafish Plasmid Collection

    Type
    Collection
    ...days), short generation time (~3 months), ease of care and breeding, and transparency of the embryo and...destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and...

  11. Church Lab CRISPR Plasmids

    Type
    Collection
    ...Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science . 2013 Jan ...Saccharomyces cerevisiae using CRISPR-Cas systems. Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church...

  12. Viral Production

    Type
    Collection
    ...line is maintained for ~20 passages before being discarded and replaced with a new vial of early passage ...produced in the line will be taken off-line and discarded. Confirmation of Transfer Plasmid Addgene uses...

  13. TALEN Plasmids and Kits

    Type
    Collection
    ...Gate TALEN 2.0 38142 RCIscript-GoldyTALEN Daniel Carlson The plasmids pC-GoldyTALEN and RCIsricpt-GoldyTALEN..., and G) and the full length human LSD1 at the carboxy-terminus of the protein. 49043 EMM67 49044 EMM71T...

  14. Chemogenetics Plasmids

    Type
    Collection
    ...(Gq), rMD3(Gs), and hMD4(Gi) are all based on muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins... tdTomato No Dimidschstein 111397 pAAV-hSyn-DIO {hCAR}off-{hM4Di-mCherry}on-W3SL hM4D (Gi) Syn mCherry...

  16. Plan Your Experiment

    Type
    Collection
    ...sure to check whether reagents are available to carry out your experiment in your particular model organism...save your lab valuable time and resources when carrying out CRISPR experiments. Read more about how to...

  17. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...site. Design additional primers to analyze for scarring (small indels produced at the sgRNA cleavage site...to amplify the sgRNA target site to examine for scarring. This may be useful to characterize the non-deleted...

  18. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library 82480 Knockout Mouse Xie N/A N/A 395 CRISPRa...Knockout Human Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library...

  19. Genetic Code Expansion

    Type
    Collection
    ...126035 pEVOL-ABK PylRS M. barkeri 3’-azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea Musacchio 127411...pRSF-ChPylTMSK ChPylTMSK N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK) Bacterial TAG Thomas Huber 163916...
Showing: 61 - 80 of 625 results