...such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common methods...

...EGFP reporter gene ( Reyon & Tsai et al., Nat Biotechnol. 2012 ). The table below lists plasmids encoding...

... within this set are derived from pAdenoX from Clontech and can be used to generate adenovirus expressing...

...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...

...somatic hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed...

...immunology, biochemistry, and more. The foundational technologies enabled by this collection include polymerase...

...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR - or restriction... to an open UV box (be sure to wear proper UV protection - especially for your eyes!), remove it from ...the desired DNA fragment from the gel. Notes: To protect the UV box, it is a good idea to place the gel ...will not significantly reduce the UV, but will protect the UV box from being cut by the razor blade. Try...

...laboratory animals and human tissue specimens. These technologies have the potential to transform neuroscience...

...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...

...between different types of hazards and to provide protective measures for each level, as each level has different...Equipment Lab coat Close-toed shoes Gloves Eye protection or face shields Eye wash Safety shower Sharps... entering the lab, put on the proper personal protective equipment (PPE) and wear it the whole time you...re doing your work in the safest way possible. Protecting yourself and working smart in the lab is important...

...Introduction Immunocytochemistry is a technique that uses antibodies to detect antigens in cells. Here we describe...µL of the 5 mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution... the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells ...

...Western blot is a technique used to separate proteins by size followed by detection using antibodies specific...gel imager with chemiluminescence detection or a dark room to detect the bands. Tips and Troubleshooting...

...CRISPR in your lab. Learn More Guide to CRISPR technology Addgene Blog Posts How to Design Your gRNA for...

...Bacterial Transformation Summary The following technique can be used to easily move any piece of DNA from... by restriction digest is a commonly used lab technique. For the purposes of this tutorial we will discuss...from one plasmid to another. However, the same technique can be used to move promoters, selectable markers...

...for a stock mixture is an essential laboratory technique, as imprecise measurements can affect the outcome...weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to handling the materials...

...GAGTGGGAGGGTCCCGTCCT 68060 cut S. pyogenes Fungal Biology and Biotechnology 2015, 2:4 Hong Ctnnb1 M. musculus AGCTCCTTCCCTGAGTGGCA...

...insert and vector backbone are known and thus this technique can be quickly used to verify your plasmid. The... should be. A useful restriction enzyme based technique for verifying plasmids like this is "plasmid fingerprinting...

...Microfuge tubes or other suitable container Personal protective equipment (PPE) appropriate for the biosafety...higher speeds and are used for more specialized techniques. Regardless of the type of centrifuge you use...

...ET SSB) to the isothermal reaction mix. ET SSB protects 3’ ssDNA ends from the ssDNA-specific endonuclease... is to “stitch” together several oligos. This technique is especially useful for introducing promoters...

...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to revolutionize...

...advantage of the physical properties of light to detect small objects. Two of the most important properties...Procedure Set up your microscope by removing any protective cover that may be on it and plugging in the power...

...EtBr is a known mutagen. Wear a lab coat, eye protection and gloves when working with this chemical. Note... damage to the DNA. Note: When using UV light, protect your skin by wearing safety goggles or a face shield...

...the literature for an appropriate sterilization technique if you are working with any weird and wonderful... into your ~60 ℃ molten gel mix using sterile technique. Swirl the agar bottle to ensure even distribution...

... able to distribute materials containing FLEx technology. Read the genOway press release for more information...

...preparing and handling lentiviral particles. Personal protective clothing should be worn at all times. Use plastic...gene delivery in vivo. Zufferey R et. al. Nat Biotechnol . 1997. 15(9):871-5. PubMed . Self-inactivating...

...do NOT dig into the agar. Another very popular technique is to draw in discontinuous lines. Start by streaking...

...MCS of an empty vector. However, the following technique can be used just as easily to add short tags or...

...digestion is commonly used in molecular cloning techniques, such as PCR or restriction cloning . It is also...

...Bacterial Culture Introduction Many molecular biology techniques require highly purified and concentrated plasmid...

...between the oligo primers. This molecular biology technique creates several micrograms of target DNA from ...