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Showing: 761 - 780 of 1261 results
  1. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post
    ... this stage and that can be used to simulate in silico cloning. This will definitely help in understanding...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...
  2. Plasmid Tools for Microbiome Studies

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    Blog Post
    ...modular suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow ...Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad host-range...
  3. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...
  4. Viral Vectors 101: Chemogenetics

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    Blog Post
    ...important to consider that the same receptor can elicit different responses depending on what cell type...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...
  5. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...
  6. Hot Plasmids - October 2020

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    Blog Post
    ...Jerome Goudeau, Cynthia Kenyon, and colleagues at Calico Life Sciences. Like many split FPs, split-wrmScarlet...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...
  7. The Codon Guide to Synthetic Biology

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    Blog Post
    ...from Bruce Wetzel and Harry Schaefer, NCI, NIH | License   I’m updating it regularly as people send me suggestions...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...
  8. New Videos: Addgene Lab Tips

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    Blog Post
    ...trick. Just flip, and press! Lab tip #2: Tube flick trick When combining reagents in the lab, it is ...the various reagents to a single tube and simply flick the tube several times. This will help ensure ensure...
  9. Pooled Library Amplifications

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    Blog Post
    ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...containing smaller, recombined backbones that may replicate faster. For this reason, if your library has inserts...
  10. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post
    ... into Addgene’s CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution...support for, this and previous Addgene CRISPR publications.  References and resources References Pyhtila...
  11. Antibody Data Repositories and Search Engines

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    Blog Post
    ... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...
  12. Call for Guest Bloggers

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    Blog Post
    ...minipreps? If so, we’d love to have you write for us! Click Here to Contribute to the Addgene Blog Possible ...molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development...
Showing: 761 - 780 of 1261 results