...Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts the ... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

... ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses....guide RNA / shRNA inserts Resistance Marker: This indicates the selectable marker used in your model system...

...study by the NSF showed that among US 4th graders tested on the Science National Assessment of Educational... you don’t have children but are interested in testing your SciComm skills or are interested in a career...

...within the folded protein (green region). Methanol dehydrates the protein, which can disrupt protein structure...at your antibody datasheet to see if it has been tested in IF and if a protocol is available. When possible...

...longer educational videos, and Twitter, for sharing updates, new deposits, and new blog posts!    How to... Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication should be...

...results in preclinical studies some potential candidates for rare to ultra-rare ocular diseases are being... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

... on ice (approximately 20-30 mins). Remove agar plates (containing the appropriate antibiotic ) from storage...that there isn't too much liquid media on the agar plates. If the agar plate doesn't dry adequately before...the liquid and won't grow in colonies. Incubate plates at 37°C overnight. Tips and FAQ How can I save ...access to an electroporator and the appropriate cuvettes. Follow the manufacturer's instructions for each...

...others understand ours. Improving access to data promotes dissemination, especially for young researchers...useful to others and efforts to enhance these attributes should be welcomed. However, expectations must...

...for labs and nonprofits, who tend to prioritize candidates that are excited about their work and mission... will be close to that of a professional email. Notes from real-life hiring managers In preparation for...

... Jennifer Lippincott-Schwartz at the National Institutes of Health and he is currently an Associate Professor...Paladino, Simona, et al. "Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored...

...checked circle. Typically, a yellow check mark indicates that a particular parameter was automatically ...sequencing bases downstream of the cut. Summary ICE generates NGS-quality CRISPR editing analysis from Sanger...

...transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates. Blood 100:823–832...

...particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie, Center...assay. This may be due to competition for binding sites between the primers and the AAV genome repeats. ...

...of recruiters for identifying appropriate job candidates. If you do receive such a call, make sure to ...-out-of-job-fairs-2014-3 https://www.forbes.com/sites/neilpatel/2015/05/21/15-surprising-rules-you-should-know-regarding-linkedin-etiquette...

...preclinical and clinical research to discover and test the best new therapeutics. Consultants often work...PhDs, and an increasing recognition of the value graduates bring to these roles. Healthcare consulting is...

...lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one ... plate in DMEM Complete in 10 cm tissue culture plates. Incubate the cells at 37 °C, 5% CO 2 for ~20 h...the table below for a possible range of ratios to test: Ratio of DNA:PEI μg of DNA μL of 1 mg/mL PEI 1:...Centrifuge the viral supernatant at 2100 rcf for 5 minutes to pellet any packaging cells that were collected...

...Biosafety Cabinet 4 °C Refrigerator Micropipettes and tips Pipettor and pipettes Benchtop centrifuge compatible...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...Reagent Preparation instructions. Wipe down all pipettes and equipment with 10% bleach prior to use. Reagent...

...of toxins before the second crossover step, they tested their expression under both rhamnose- and arabinose-inducible...selective RNA unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. ...

...doesn’t affect any endogenous restriction enzyme sites? Never fear! You can introduce a silent, passenger...CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing you...

...why they are paid relatively low wages (Stephan estimates that postdocs are paid $16.15/h before fringe ...and Graduate Student Career Outcomes University websites do not currently publish career outcomes for PhDs...