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Showing: 741 - 760 of 791 results

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Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab now available as a ready-to-use viral prep ...
3D Printing Meets CRISPR Cas9

Type

Blog Post

...colleagues to reduce off-target hits in a rationally engineered Cas9 with improved specificity. Hold this flexible...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...
Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

...of endA+ strains Strains like Stbl3 have been engineered to reduce plasmid recombination, but are also...
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Type

Blog Post

...that provides a community for biologists and bioengineers to share their educational materials, protocols...
Your Lentiviral Plasmid FAQs Answered

Type

Blog Post

...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and...
Negotiating Work and Life: How to Find the Joy

Type

Blog Post

...your plan to live a good life may be the most challenging assay you will ever have the opportunity to develop...
A Career in Grants: How To Become a Grant Professional

Type

Blog Post

...Becoming a grant professional is rewarding and challenging! Jo Miller, GPC, CSMS is the founder and principal...
Getting to Know Expansion Microscopy

Type

Blog Post

...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...
Simplify Cloning with in vivo Assembly

Type

Blog Post

...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp...
Plasmids 101: Colony PCR

Type

Blog Post

...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...
Better Dyeing Through Chemistry & Small Molecule Fluorophores

Type

Blog Post

...motif attached to a fluorophore (Figure 1b). Engineered ligases (e.g., lipoic acid ligase, biotin ligase...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

...beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing techniques function...
Plasmids 101: The Promoter Region – Let's Go!

Type

Blog Post

...alcohol dehydrogenase I Repressed by ethanol Full length version is strong with high expression. Truncated...
Hot Plasmids Spring 2024

Type

Blog Post

...This is similar to the proposed mechanism for engineered pegRNAs (epegRNAs), which add a structural motif...
Tips for Writing a Good Cover Letter

Type

Blog Post

...skills and experiences fit the job requirements, strengthened an application. Even so, there were examples...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

...Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa. Financial and moral supporters Denise Akob, ...
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Type

Blog Post

...blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity...

Showing: 741 - 760 of 791 results

Results per page:

Hot Plasmids and Viral Preps - May 2021

3D Printing Meets CRISPR Cas9

Cloning Mammalian Cells with the Agarose Method

Plasmids 101: Optimizing Plasmid Yields

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Your Lentiviral Plasmid FAQs Answered

Negotiating Work and Life: How to Find the Joy

A Career in Grants: How To Become a Grant Professional

Getting to Know Expansion Microscopy

Simplify Cloning with in vivo Assembly

Plasmids 101: Colony PCR

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Better Dyeing Through Chemistry & Small Molecule Fluorophores

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Plasmids 101: The Promoter Region – Let's Go!

Hot Plasmids Spring 2024

Tips for Writing a Good Cover Letter

Plasmids 101: Broad Host Range Plasmids

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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