Skip to main content
Addgene
Showing: 721 - 740 of 934 results

  1. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...sequence. Co-delivery of wild-type Cas9 and a gRNA generates a double-strand break in the target DNA, which...experiment, the use of appropriate controls, multiple replicates and several cell types can strengthen your results...

  2. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...biology of the worm into a computer model that recapitulates how molecular biology impacts worm locomotion...generally funded on a membership model - everyone contributes each month - with only occasional supplementation...

  3. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...immunogenicity of AdVs is what makes them ideal candidates for applications in oncolysis and vaccination...by recombination. It is therefore recommended to test for the presence of RCA in the viral stock by doing...

  5. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...using much smaller regions of homology, which facilitates easier cloning. Abbreviated protocol for PITCh... by CRISPR design tools at crispr.mit.edu. They tested their system in HEK293 cells, integrating the GFP-Puro...

  8. Tips for Technical Support Calls

    Type
    Blog Post
    ...number of messages in our thread or remove a good 5 minutes from our phone call. If your concern is based...get. There are questions that are answerable in minutes and questions that could take weeks to resolve ...

  9. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...virus in Brazil that was correlated with greater rates of infection and rare, but severe, symptoms, including...unlike most research, but the urgent need for the test did catalyze our resolve to get the work done as...

  10. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...0.22 µm filter) or by a centrifugation step (5 minutes at 300-500 g). After removing packaging cell debris...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias ...

  12. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ...comparing different isoforms. In addition to our favorites, there are a number of sequence alignment tools...

  13. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... compared to other systems because the system modulates protein post-translationally, unlike many other...through interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in ...

  14. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...inside and outside your field. Your colleagues, lab mates, and PI can provide feedback on your technical presentation...pressed for time, limit yourself to five to ten minutes per poster. Ask the presenter to give the “elevator...

  15. PhD Applications After COVID

    Type
    Blog Post
    ...applied to PhD programs in Biology around the United States in 2021, I know that this process only became even...program you’ll be using for the interview. Having notes close to your computer with things you want to highlight...

  16. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...using a pinhole.  However, the excitation light excites all of the fluorophores as it passes through the...the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample surrounding...

  18. Transferable Skills: Negotiation

    Type
    Blog Post
    ...(Can I attend this conference?) and with my lab mates on which lab jobs needed to be done (Who is responsible...your needs actually are: Maybe you only need 15 minutes of the two hour time slot, so she can take the ...
Showing: 721 - 740 of 934 results