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We narrowed to 958 results for: tes

Showing: 721 - 740 of 958 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...below): 1. Resistant cells among the separate replicates would contain the same labels - i.e. they would...subpopulation 2. Resistant cells among the separate replicates would have different labels - i.e. they would...

  3. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...restriction sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends...as long as it is already bounded by restriction sites that are also present in the same orientation on...Analyzer , allow you to identify which restriction sites are present in a given sequence. When selecting ...recipient plasmid as well as a specifically designed test digest later to verify that the insert was cloned...a version of your insert flanked by restriction sites compatible with the recipient plasmid's MCS. However... within your insert. Adding desired restriction sites to your recipient plasmid : You can modify the MCS...

  4. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...dilution is appropriate). Quality of duplicates: Exclude duplicates from analyses if there is more than...-log of the expected titer of the samples being tested. Always include a No Template Control (NTC), i....small aliquot of each standard (enough for 1 or 2 plates) and store at -20 °C. Once a standard is thawed...the assay multiple plasmids containing ITR were tested. Plasmid #59462 is one plasmid that gave reliable...consistent results. Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs...second peak at a temperature of ~70–75 °C usually indicates the presence of primer dimers which can increase...

  5. A Look at Addgene's QC Process

    Type
    Blog Post
    ...the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature...at a best practices meeting, where the QC team updates and reviews our sequencing QC standards to ensure...

  6. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other plasmids...undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be small and poorly ...

  8. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...immunogenicity of AdVs is what makes them ideal candidates for applications in oncolysis and vaccination...by recombination. It is therefore recommended to test for the presence of RCA in the viral stock by doing...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...transfer plasmid, the component that physically integrates into the host genome, the virus is replication...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate in the waste...

  11. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...clones and thus the potential to bind many different sites on the target protein they tend to have a strong...and bind to your primary antibody. Manning et al. tested a variety of commercially available secondary antibodies...

  13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...prohibitively expensive for many labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag... few single base pair mutations in crRNA target sites found within the homology areas of the plasmid to...

  14. Tips for Technical Support Calls

    Type
    Blog Post
    ...number of messages in our thread or remove a good 5 minutes from our phone call. If your concern is based...get. There are questions that are answerable in minutes and questions that could take weeks to resolve ...

  15. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...virus in Brazil that was correlated with greater rates of infection and rare, but severe, symptoms, including...unlike most research, but the urgent need for the test did catalyze our resolve to get the work done as...

  16. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...0.22 µm filter) or by a centrifugation step (5 minutes at 300-500 g). After removing packaging cell debris...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias ...

  17. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...biology of the worm into a computer model that recapitulates how molecular biology impacts worm locomotion...generally funded on a membership model - everyone contributes each month - with only occasional supplementation...

  20. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ... fits the plate. Incubate plates at 37 ℃ for 18 hours. Day 2 Observe plates for colony formation. Shown...Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA...source) Incubator Reagents 6 cm diameter LB/agar plates without antibiotic High concentration (100 mg/mL... the plate at room temperature for at least 30 minutes with the lid on to give the antibiotic time to ... with smaller size than the 1 mg/mL and 2 mg/mL plates and effective selection. Selection Curve of Transformed...
Showing: 721 - 740 of 958 results