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We narrowed to 955 results for: tes

Showing: 721 - 740 of 955 results

  1. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...immunogenicity of AdVs is what makes them ideal candidates for applications in oncolysis and vaccination...by recombination. It is therefore recommended to test for the presence of RCA in the viral stock by doing...

  3. A Look at Addgene's QC Process

    Type
    Blog Post
    ...the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature...at a best practices meeting, where the QC team updates and reviews our sequencing QC standards to ensure...

  4. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other plasmids...undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be small and poorly ...

  5. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...prohibitively expensive for many labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag... few single base pair mutations in crRNA target sites found within the homology areas of the plasmid to...

  6. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...clones and thus the potential to bind many different sites on the target protein they tend to have a strong...and bind to your primary antibody. Manning et al. tested a variety of commercially available secondary antibodies...

  9. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...biology of the worm into a computer model that recapitulates how molecular biology impacts worm locomotion...generally funded on a membership model - everyone contributes each month - with only occasional supplementation...

  12. Optogenetics Guide

    Type
    Guide
    ...Experiment References The field of optogenetics integrates optics and genetic engineering to manipulate ...photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent amplitude... msec with hChR2 to "long lasting" (seconds to minutes) with stable step-function opsins (SSFOs). Delivery...flavin mononucleotide as its chromophore, and phosphorylates the transcriptional activator FixJ in the absence...

  13. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...restriction sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends...as long as it is already bounded by restriction sites that are also present in the same orientation on...Analyzer , allow you to identify which restriction sites are present in a given sequence. When selecting ...recipient plasmid as well as a specifically designed test digest later to verify that the insert was cloned...a version of your insert flanked by restriction sites compatible with the recipient plasmid's MCS. However... within your insert. Adding desired restriction sites to your recipient plasmid : You can modify the MCS...

  16. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... compared to other systems because the system modulates protein post-translationally, unlike many other...through interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in ...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...also enabled imaging of their propagation along dendrites for the first time (Figure 3). The team also used...a patient with non-neurodegenerative diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024...

  18. PhD Applications After COVID

    Type
    Blog Post
    ...applied to PhD programs in Biology around the United States in 2021, I know that this process only became even...program you’ll be using for the interview. Having notes close to your computer with things you want to highlight...

  19. Transferable Skills: Negotiation

    Type
    Blog Post
    ...(Can I attend this conference?) and with my lab mates on which lab jobs needed to be done (Who is responsible...your needs actually are: Maybe you only need 15 minutes of the two hour time slot, so she can take the ...
Showing: 721 - 740 of 955 results