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We narrowed to 965 results for: tes

Showing: 721 - 740 of 965 results

  1. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...load The Wikipedia definition of cognitive load states: “cognitive load refers to the used amount of working...even the most enthusiastic of learners.  What contributes to cognitive load?  Everything! Here are some...

  2. Addgene: The First Twenty Years

    Type
    Blog Post
    ...theaters, the Razr was the hottest phone on the market, and two new websites, Facebook and Gmail, were ...

  3. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough for this approach... guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik....

  4. The time and cost required to make a plasmid

    Type
    Blog Post
    ...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...

  5. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...idea of the basics of immunoimaging. A scientist conjugates an antibody with a signaling molecule, the antibody...secondary antibodies on hand and use them to easily test different antibodies or different signaling molecules...

  6. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...weeks to increase the antibody titer. The titer is tested following each immunization until the desired level...

  8. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...below): 1. Resistant cells among the separate replicates would contain the same labels - i.e. they would...subpopulation 2. Resistant cells among the separate replicates would have different labels - i.e. they would...

  10. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ... fits the plate. Incubate plates at 37 ℃ for 18 hours. Day 2 Observe plates for colony formation. Shown...Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA...source) Incubator Reagents 6 cm diameter LB/agar plates without antibiotic High concentration (100 mg/mL... the plate at room temperature for at least 30 minutes with the lid on to give the antibiotic time to ... with smaller size than the 1 mg/mL and 2 mg/mL plates and effective selection. Selection Curve of Transformed...

  11. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...immunogenicity of AdVs is what makes them ideal candidates for applications in oncolysis and vaccination...by recombination. It is therefore recommended to test for the presence of RCA in the viral stock by doing...

  13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...prohibitively expensive for many labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag... few single base pair mutations in crRNA target sites found within the homology areas of the plasmid to...

  15. A Look at Addgene's QC Process

    Type
    Blog Post
    ...the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature...at a best practices meeting, where the QC team updates and reviews our sequencing QC standards to ensure...

  16. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other plasmids...undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be small and poorly ...

  17. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...transfer plasmid, the component that physically integrates into the host genome, the virus is replication...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate in the waste...

  18. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...clones and thus the potential to bind many different sites on the target protein they tend to have a strong...and bind to your primary antibody. Manning et al. tested a variety of commercially available secondary antibodies...

  20. Tips for Technical Support Calls

    Type
    Blog Post
    ...number of messages in our thread or remove a good 5 minutes from our phone call. If your concern is based...get. There are questions that are answerable in minutes and questions that could take weeks to resolve ...
Showing: 721 - 740 of 965 results