Addgene

We narrowed to 791 results for: eng

Showing: 721 - 740 of 791 results

Results per page:

Antibodies 101: Choosing the Right Isotype

Type

Blog Post

...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide...
Genome-wide Screening Using CRISPR

Type

Blog Post

...multiple replicates and several cell types can strengthen your results. Enrichment or depletion of multiple...
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

Type

Blog Post

...features that allow such “chemical rescue” in vivo. Engineering the human disease alleles into cells for testing...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...
Educational Planarian Tools for Teaching Developmental Biology Techniques

Type

Blog Post

...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...
Antibodies 101: Stripping and Reprobing Western Blots

Type

Blog Post

...time you strip a blot, you should not compare the strength of bands from different rounds of detection. Finally...
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Type

Blog Post

...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Type

Blog Post

...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...
6 Tips for Grant Writing

Type

Blog Post

...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...
Adapting Toehold Switches to Detect Zika Virus

Type

Blog Post

...2014, as postdoctoral fellows with Jim Collins and Peng Yin at the Wyss Institute (Harvard University), ...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...included in the search to maximize the alignment length. While this recommendation is not infallible, we...
Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...
Of Myc and Men

Type

Blog Post

...making the antibody more amenable to protein engineering. As a result, you can now find Myc1-9E10 in a...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983...
Tips to Make the Most of a Scientific Conference

Type

Blog Post

.... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...
10 Basic tips for mammalian cell culture

Type

Blog Post

...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...
Experimenting with New Careers while in Grad School

Type

Blog Post

...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding...
Viral Vectors 101: Producing Your rAAV

Type

Blog Post

...Ceballos-Diaz, C., Liu, X., Paterno, G., Mejia, C., Deng, D., Menezes, K., Londono, L., Arjona, K., Parianos...
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Type

Blog Post

...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

Showing: 721 - 740 of 791 results

Results per page:

Antibodies 101: Choosing the Right Isotype

Genome-wide Screening Using CRISPR

BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

Adenoviral Vector Production and Troubleshooting

Educational Planarian Tools for Teaching Developmental Biology Techniques

Antibodies 101: Stripping and Reprobing Western Blots

A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

6 Tips for Grant Writing

Adapting Toehold Switches to Detect Zika Virus

Tips for Using BLAST to Verify Plasmids

Twenty Years of Addgene Sharing: CRISPR

Of Myc and Men

Light Sheet Fluorescence Microscopy

Tips to Make the Most of a Scientific Conference

10 Basic tips for mammalian cell culture

Experimenting with New Careers while in Grad School

Viral Vectors 101: Producing Your rAAV

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Polymerase Chain Reaction Overview and Applications

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections