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...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...

...time you strip a blot, you should not compare the strength of bands from different rounds of detection. Finally...

...Chemogenetics relies on genetically introducing engineered receptors that are only activated by otherwise...

...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide...

...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...

...2014, as postdoctoral fellows with Jim Collins and Peng Yin at the Wyss Institute (Harvard University), ...

...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

.... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...

...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...

... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....

...Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox...

...Ceballos-Diaz, C., Liu, X., Paterno, G., Mejia, C., Deng, D., Menezes, K., Londono, L., Arjona, K., Parianos...

... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

...making the antibody more amenable to protein engineering. As a result, you can now find Myc1-9E10 in a...

...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

...(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab now available as a ready-to-use viral prep ...

...colleagues to reduce off-target hits in a rationally engineered Cas9 with improved specificity. Hold this flexible...

...of endA+ strains Strains like Stbl3 have been engineered to reduce plasmid recombination, but are also...