Addgene

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Kiran Musunuru on the Newest TALEN Genome-Editing System

Type

Blog Post

...genomes of mammalian or other cells. TALENs are engineered proteins that comprise a DNA binding domain and...
RNA Extraction Without A Kit

Type

Blog Post

...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Type

Blog Post

...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Type

Blog Post

...Galvan et al., 2019). The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer...
Supporting Rare Disease Research with the 2020 BeHEARD Awards

Type

Blog Post

... Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This...
RaPID Detection of RNA-protein Interactions

Type

Blog Post

...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...
Summer SciComm: Preprints

Type

Blog Post

...and feedback giving authors the opportunity to strengthen the manuscript prior to submission. Preprints...
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Type

Blog Post

...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...
Reproducibility for Everyone: Lessons from an Open Science Collaboration

Type

Blog Post

...working with communities, not for them - Another strength of this collaboration is that it is driven by ...
Viral Vectors 101: An Introduction to AAV

Type

Blog Post

...and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector genome of both polarities are rescued...
Transferable Skills Guide: Public Speaking

Type

Blog Post

...gives your entire audience a chance to comfortably engage with you. If you can give a seminar, lead a journal...
The time and cost required to make a plasmid

Type

Blog Post

...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...
Plasmids 101: The protein expression toolbox

Type

Blog Post

...promoters Tetracycline-dependent promoters are engineered by placing tet response elements upstream of ...
Human Germline Editing Using CRISPR

Type

Blog Post

...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Type

Blog Post

...new plasmids and/or cells or organisms that are engineered can be shared to simplify the comparison by others...
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Type

Blog Post

...~6.5 - 9 *Optimal sequential excitations wavelengths as determined by Rosado et al: see Table 2 for...
Developing Lab Management Software for Biology

Type

Blog Post

...in on the fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software...
Promoters

Type

Guide

...into a protein. RNA polymerase II transcribes messenger RNA (mRNA) which is the RNA responsible for providing...
Antibodies 101: Stripping and Reprobing Western Blots

Type

Blog Post

...time you strip a blot, you should not compare the strength of bands from different rounds of detection. Finally...
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Type

Blog Post

...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...

Showing: 701 - 720 of 791 results

Results per page:

Kiran Musunuru on the Newest TALEN Genome-Editing System

RNA Extraction Without A Kit

Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Supporting Rare Disease Research with the 2020 BeHEARD Awards

RaPID Detection of RNA-protein Interactions

Summer SciComm: Preprints

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Reproducibility for Everyone: Lessons from an Open Science Collaboration

Viral Vectors 101: An Introduction to AAV

Transferable Skills Guide: Public Speaking

The time and cost required to make a plasmid

Plasmids 101: The protein expression toolbox

Human Germline Editing Using CRISPR

Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Developing Lab Management Software for Biology

Promoters

Antibodies 101: Stripping and Reprobing Western Blots

A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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