...short, K. phaffii is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic...has been sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary...

...layer of both bacteria types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively...the cell purple. When the alcohol is added, it dehydrates the peptidoglycan layer, trapping the large dye...

...nearby resulting in fluorescence within tens of minutes (Cabantous et al., 2013). The Shu Lab took advantage...reporter can be used to visualize apoptosis of enterocytes in the midgut of Drosophila, an observation that...

... all worked in tandem, within their respective mandates, to tackle a controversial and ethically fraught...stages of human clinical trials. This model also demonstrates that when we are at the point of scientifically...

...on a web-based interactive visualization of FP attributes (which turned into www.fpvis.org).  That page...is also difficult to scale such a resource when updates depend on a single person. With thousands of scientists...

...dynamically rearranging into different polymerization states. Instead, R body monomers respond to pH changes...their cell walls removed with lysozyme). This demonstrates that R bodies can function to break membranes...

...Pomodoro technique, which is when you spend 25 minutes focusing on a task, like writing, and then take...managers, but Mendeley is free to use. You can take notes and highlight the papers saved to your Mendeley ...

...under a new promoter each time a new hypothesis is tested. Controlling multiple genes with Gal4/UAS system...Targeted gene expression as a means of altering cell fates and generating dominant phenotypes., Development...

...Addgene plasmids and those without, we adjust our estimates based on the journal that published the articles... This is also true for other journals, so our estimates now take into account any differences in citations...

... of a 96-well plate. Then, samples from several plates are pooled together to create a single library ... from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped to seqWell, where...

...writing skills Writing a thesis or dissertation demonstrates that you have advanced writing and formatting...apply for a job, it is useful to search online job websites to see what requirements or qualifications are...

...alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select lentiviral... vectors from the repository for you. We perform test infections with all of our viral preps and host ...

... of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can ... cartoon, in which we are adding EcoRI and NotI sites to Your Gene of Interest (YGOI) for ligation into...amplified (usually 18-21bp). When selecting restriction sites, you should use a DNA analysis tool, such as Addgene...Analyzer , to allow you to identify which restriction sites are present in a given sequence. You want to choose...based cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1...

...activate all 20,000 genes, it is not possible to test each one and make sure it’s working. You can only...resistant to treatment with a BRAF inhibitor. (The test was the perfect complement for one conducted earlier...

...supernatant through each layer. The right panel illustrates a gradient tube before and after ultracentrifugation...ultracentrifugation. The viral supernatant migrates through increasingly dense layers until the macromolecule density...

...engineering projects in a very big way. In one of his latest papers, published in Molecular Systems Biology ...that took a spaceship to the moon was built on NOR gates. It’s a fundamental unit. We did demonstrate that...

... including the recent homepage and search tool updates. Now, let’s take a look at the least visible part...behind Addgene’s new search  Like millions of other websites, we built our new search engine atop Elasticsearch...

...security-critical bugs, and contribute to constant updates of the software. In hardware, this process is often...are as follows: Detailed documentation, which facilitates the reproduction of the structure in a similar...

...small molecule realm is typically not feasible to test in any given screening experiment. Thus, a limited...require there to be a culture condition which eliminates manipulations that do not affect the function...

...makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen Elledge. His new ...fragments without the need for specific restriction sites or DNA ligase! This post was originally written...