We narrowed to 956 results for: tes

...under a new promoter each time a new hypothesis is tested. Controlling multiple genes with Gal4/UAS system...Targeted gene expression as a means of altering cell fates and generating dominant phenotypes., Development...

...Addgene plasmids and those without, we adjust our estimates based on the journal that published the articles... This is also true for other journals, so our estimates now take into account any differences in citations...

... of a 96-well plate. Then, samples from several plates are pooled together to create a single library ... from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped to seqWell, where...

... all worked in tandem, within their respective mandates, to tackle a controversial and ethically fraught...stages of human clinical trials. This model also demonstrates that when we are at the point of scientifically...

...writing skills Writing a thesis or dissertation demonstrates that you have advanced writing and formatting...apply for a job, it is useful to search online job websites to see what requirements or qualifications are...

...alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select lentiviral... vectors from the repository for you. We perform test infections with all of our viral preps and host ...

...dynamically rearranging into different polymerization states. Instead, R body monomers respond to pH changes...their cell walls removed with lysozyme). This demonstrates that R bodies can function to break membranes...

... up on a Saturday at like 7 am to go check your plates and you’re excited to do so, you know that that...Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,...

...enzymes which cleave the DNA molecules at specific sites and 2) running the reaction on an agarose gel to...To do this, you'll use enzymes with restriction sites that flank the insert. You will need to know both...

...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...instead of room temperature or 37 °C. The protocol notes when there are different incubation options available...empirically determined. If possible, run an initial test with serially diluted primary antibody and determine...Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette 2–20 µL single channel...VWR 76322-150 Pipette tips, 20 µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, ...display a green product. A precipitated product indicates the presence of too much HRP and the need to optimize... and incubated overnight with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary...

...parts: the receptor itself and the G-proteins it associates with.  Receptor: GPCRs are also known as seven...transducing receptors in the brain they are ideal candidates for use in chemogenetics. To create chemogenetics...

...small molecule realm is typically not feasible to test in any given screening experiment. Thus, a limited...require there to be a culture condition which eliminates manipulations that do not affect the function...

...  Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s...celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though...

...2: comparison of Cas 9 vs. Cas 12a expression cassettes when encoding multiple guides. While two guides... and fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering...

...sequence containing transcription factor binding sites can increase transgene expression and transduction...validate ITR sequences as these enzymes cut at known sites within the ITR. However, small deletions and point...

...security-critical bugs, and contribute to constant updates of the software. In hardware, this process is often...are as follows: Detailed documentation, which facilitates the reproduction of the structure in a similar...

... including the recent homepage and search tool updates. Now, let’s take a look at the least visible part...behind Addgene’s new search  Like millions of other websites, we built our new search engine atop Elasticsearch...

...site of interest, which nCas9 nicks and then dissociates from. PolI3M then binds the nicked DNA, and extends..., where it nicks its target DNA (left). Cas9 dissociates and PolI binds (middle) and extends from the ...

...makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen Elledge. His new ...fragments without the need for specific restriction sites or DNA ligase! This post was originally written...

...concluded “Aspirin promotes autophagy, inhibits platelet aggregation, and potentiates the effect of PD-...