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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 958 results for: tes

Showing: 681 - 700 of 958 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...instead of room temperature or 37 °C. The protocol notes when there are different incubation options available...empirically determined. If possible, run an initial test with serially diluted primary antibody and determine...Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette 2–20 µL single channel...VWR 76322-150 Pipette tips, 20 µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, ...display a green product. A precipitated product indicates the presence of too much HRP and the need to optimize... and incubated overnight with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary...

  2. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...layer of both bacteria types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively...the cell purple. When the alcohol is added, it dehydrates the peptidoglycan layer, trapping the large dye...

  4. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...sequence containing transcription factor binding sites can increase transgene expression and transduction...validate ITR sequences as these enzymes cut at known sites within the ITR. However, small deletions and point...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...short, K. phaffii is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic...has been sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary...

  11. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Addgene plasmids and those without, we adjust our estimates based on the journal that published the articles... This is also true for other journals, so our estimates now take into account any differences in citations...

  12. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ... of a 96-well plate. Then, samples from several plates are pooled together to create a single library ... from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped to seqWell, where...

  13. Science Career Options

    Type
    Blog Post
    ...writing skills Writing a thesis or dissertation demonstrates that you have advanced writing and formatting...apply for a job, it is useful to search online job websites to see what requirements or qualifications are...

  14. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select lentiviral... vectors from the repository for you. We perform test infections with all of our viral preps and host ...

  15. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...dynamically rearranging into different polymerization states. Instead, R body monomers respond to pH changes...their cell walls removed with lysozyme). This demonstrates that R bodies can function to break membranes...

  16. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ... up on a Saturday at like 7 am to go check your plates and you’re excited to do so, you know that that...Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,...

  18. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...reaction requiring luciferin, ATP and oxygen as substrates. Production of photons by this bioluminescent...of Photon Generation Slower Faster Cofactors/Substrates Required Not Required Signal Strength Lower...

  19. Antibodies 101: ChIP

    Type
    Blog Post
    ...  Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s...celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though...

  20. Hot Plasmids - February 2022

    Type
    Blog Post
    ...2: comparison of Cas 9 vs. Cas 12a expression cassettes when encoding multiple guides. While two guides... and fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering...
Showing: 681 - 700 of 958 results