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  2. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature...

  3. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... most start-up P.I.’s. It doesn’t have to be complicated, or difficult to create this list. Start BEFORE...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...that carried out the labor-intensive process of replicating your new plasmid: the Escherichia coli bacteria... Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...sequencing. While this may be unnecessary for many applications, it is the only comprehensive method available...you’ve tried everything listed here. For some applications, this is ok, but it still must be controlled...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... DNA from host cell nucleases (4). To further complicate matters, even when sequenced, phage genomes tend...Franklin L., et al. "Revisiting phage therapy: new applications for old resources." Trends in microbiology 23.4...

  8. Finding Your Perfect Job After University

    Type
    Blog Post
    ...recession. As each advertised job received many applications, employers were requesting that only scientists...from other individuals projects, this can cause conflicts and favoritism, as well as affect morale and cause...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...but, in numerous studies, optogenetics is used in slice physiology in order to directly show that, for example...22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review of neuroscience...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...and gRNAs can be inserted using Gibson Assembly or SLIC cloning methods. A BsaI-based E. coli multiplexing... gRNAs can be assembled using Gibson Assembly or SLIC cloning. gRNAs are expressed from two Drosophila...

  11. Technical Design of a Western Blot

    Type
    Blog Post
    ...original publication and ensure that it includes validation data relevant to your application — and double-check...

  15. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...meeting each other's friends and families, eating delicious food, or playing one of the giant games or indoor...

  16. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...

  17. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...Addgene care package with Addgene mugs and some delicious hot chocolate for the lab. Here are some of our...

  18. We’re Going Viral This Week!

    Type
    Blog Post
    ... information on the basics of viral vectors, applications, and some new resources available from Addgene...
Showing: 621 - 640 of 836 results