Skip to main content
Addgene
Showing: 601 - 620 of 836 results

  2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...targeting anywhere in an exon. But things get more complicated if you’d like to use homology directed repair...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

  3. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...moments. The models function as thinking tools – eliciting questions like “what does this model representt...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ...

  5. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ..., blastp, and blastx searches! Now, before you click the BLAST button, consider the Program Selection...may have noticed the “Algorithm parameters” link. Click on this link to view advanced BLAST options and ...

  6. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein...mitochondrial MDH (hMDH2) with those from human cytosolic MDH (hMDH1). They then expressed the original ...

  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

  11. Bioinformatics at Addgene

    Type
    Blog Post
    ... from depositing laboratories and associated publications, but our quality control (QC) standards have...can be easily shared with others without any complicated setup. We created, tested, and debugged each ...

  12. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...Freedom: The Expansion of Data Sharing in Research Publications Resources at Addgene.org Check out our Protocol...

  15. Lab to Office Culture Shock

    Type
    Blog Post
    ...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...

  16. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... vehicle for making research data available for public access, review, and query. Gates Open Research ... both accept deposit of data associated with publications into one of five general purpose open source...

  17. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if...

  18. Hot Plasmids - August 2020

    Type
    Blog Post
    ...protein evolution By Joanne Kamens Mateo Sanchez and Alice Ting have used one biotechnology tool to optimize...cultured and FACS sorted. Image from Mateo Sanchez and Alice Ting. Sanchez MI et al., Nat methods. 2019. https...

  20. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature...
Showing: 601 - 620 of 836 results