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  1. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...response to requirements that emerged from intensive engagement with a wide range of people at the University... The two guiding principles emerging from the engagement with the Wisconsin research community were to...

  3. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...to the right - the Reddit community is active, engaged, and has great questions! 2. The r/Science subreddit...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...

  4. Retrovirus Guide

    Type
    Guide
    ...Questions (FAQ) about γ-Retroviral Plasmids How do engineered γ-retroviruses differ from lentiviruses? There...There are a few important differences between engineered γ-retroviruses and lentiviruses. First, these viruses...

  5. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...photoreceptors (Oliinyk et al., 2019). They were engineered to incorporate BV. Their advantages are their...Verkhusha, V.V. Near-infrared fluorescent proteins engineered from bacterial phytochromes. Curr Opin Chem Biol...

  6. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive research...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

  8. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...(see Figure 2). H describes the maximum signal strength, while W results from the time that the cell spent...increase the electric current and thereby the signal strength. So, we need to adjust the voltage in the cytometer...

  9. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...used here could be an attractive strategy for engineering other enzymes. Conclusion We hope this post has...precise control over vascular endothelial barrier strength. eLife, 12, RP84364. https://doi.org/10.7554/eLife...

  10. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...sites? Never fear! You can introduce a silent, passenger edit to serve as a proxy for your edit of interest... a version of Cas9 that has been specifically engineered for reduced off target editing, such as SpCas9...

  13. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...validation PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction... absence) of that particular site with fragment length polymorphism (RFLP) analysis. In this process, ...

  15. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ..., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L..... E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric...

  16. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...Sleeping Beauty, piggyBac and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084...about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty DNA transposon...

  17. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...are currently about 5.5 million articles in the English version of Wikipedia alone. Humans have been forced...a team is a really good way of creating and strengthening those social bonds. So never mind how much better...

  18. Antibodies 101: Validation

    Type
    Blog Post
    ... signal, so you’ll need a range of expression strengths to be confident in your data. This is a good option...9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS,...

  20. Lentiviral Guide

    Type
    Guide
    ...use the HIV protein tat in order to produce full length virus from the transfer vector during the viral...This deletion abolishes transcription of the full-length virus after it has incorporated into a host cell...
Showing: 581 - 600 of 791 results