... if you take time when you need it, rather than trying to push through a state of panic. Now, if I feel...you, it’s noteworthy and memorable and awful. So try to keep some perspective. Most people won't notice...did was public speaking. If you're also nervous, try taking a few deep breaths. Then, identify the specific...

...to conduct 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid...backbone + insert, unsuccessful 2 and 3 above), you can try ligating the recipient plasmid alone in the presence...you are plating on the appropriate antibiotic and try varying the “recipient plasmid : insert ratio” in... Addgene Blog: Learn How to Verify Your Plasmid Try Out Golden Gate or SLIC Cloning Read Other Plasmids...

...science education Do you aim to reach young people? Try networking with your local education community. Get...ATJCagan or create agar art with colorful microbes? Try your hand at photography and participate in a science...Nothing prepares you better than learning on the job. Try to get an internship at a magazine or newspaper (...

...priority: location or efficiency. If you are just trying to shred a long non-coding RNA, cutting most anywhere...will be achieved. On the other hand, if you are trying to knock-in a patient mutation or need to make ...efficiency — although both are of course preferred! When trying to strike that balance, you may wonder: how close...

...non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize membranes and these...IF and if a protocol is available. When possible, try to start with conditions and reagents that have already...the only way to know if a method will work is to try it. As you embark on your next IF adventure, just...integral membrane proteins. Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org/10.1177...

...have a gene of interest but have run into a wall trying to study it? It happens. Is it an evolutionarily...stop you from embarking on a fruitless journey of trying to get males to recombine two mutations onto the...not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but he...

...looked clean, professional, and modern. Instead of trying to place all of the content above the fold, we ... of Addgene's website Resources on Addgene.org Try out the new search on Addgene's homepage ...

...solution to the problem you called about. Next, you try an email, given that this is the 21st century and... direct service you’ve come to expect from us.   Try out the Help Center! The Help Center can be found...

...in a specific role or cycle. For example, if you try to lead when you are expected to be doing, you may... (in particular your boss) that you are ready to try operating in a different role?  I think the secret...

... boss. But it does not mean shouting orders and trying to get everything done your way. According to Merriam-Webster... direct with a degree of skill, To work upon or try to alter for a purpose and To direct the professional... you will take responsibility (within reason!).  Try to buffer them from unnecessary pressure “coming ... What Makes a Good Mentor? Tips for Working in Industry and Success in Management Resources at Addgene.org...

...another industry-geared option, try the Biospace career events page. Biospace holds career events all over...necessarily need to be someone a lot older than you are; try using ResearchGate to see where other graduate students...and post-docs from your institution have gone, and try sending them a professional email asking for information...employment within industry or academia; although the ACS career fair is more industry-focused. For another...over the country; sometimes several per month, giving you a plethora of opportunities to explore! Again...

...working on, start wherever it makes sense to start and try to be as chronological as possible — if you find ..., be approachable. Engage with your audience and try to learn from them. Science is a collective endeavour...

...yourself enjoying?  To find master’s-level postings, try adding “master’s” to your favorite search term, i.e...how people describe their actual work. To do this, try looking at LinkedIn profiles of people who have masters...switch your field of work, both in academia and in industry. Advancing along a career path in these two fields...fields looks different however. In industry the career ladder is larger and there are more opportunities...Career advancement for PhDs, both in academia and industry, often means moving from the lab to a desk, although...

...experiment will likely depend on: The protein you are trying to purify The expression system If you want the...fusion proteins from being purified by GST (maybe try MBP if you are having solubility issues!). Need a...

...protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating...downstream of the open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more...

...correct plasmid, scientists would spend hours to days trying find their correct clones. Additionally, the presence...cassette within the coding region of the gene they are trying to disrupt or delete, which both inactivates the...

...questions. Talk to as many lab members as possible. Try to get them in a setting out of the lab (coffee, ...Unfortunately, a bad mentor can be bad news for anyone. Try to speak to them more than once to get a full picture...link from 2020) on this topic), for this second entry in the “Mentoring for Scientists Series”, let’s ...

...fluorescence (for some background on fluorescence, try the Introduction to Fluorescence Microscopy video...that work in one context may not work in another. Try to choose primary antibodies that have been validated...time to carefully review your options. If possible, try to select a couple of antibodies that seem promising...see associated with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these ...

...example, if your work requires specialized equipment, try finding institutes that offer shared equipment that..., instead of solely discussing numerical values, try to keep conversations focused on research relevant...needs and limitations. Everything is negotiable. Try asking for discounts, collaborations, access to internal...sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions where the...

...well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the Flowtime Technique....these tips are helpful to you no matter what you’re trying to accomplish during this period. ...