.... to understand cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile a few...are preparing and carrying out this multi-pronged attack. Inflammation and tumorigenesis The conference...

...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...reality, multiple primary antibodies can and do attach to a single target protein, so this assumption ...

... protein. These RNA molecules are vulnerable to attack by both cytoplasmic and nuclear nucleases, as they...group of the ribose to protect it from nucleolytic attack. Another stabilizing sugar modification, found ...

... used to turn “on” and “off” MEK1 activity by attaching two dimerizing photoswitchable Dronpa (dron= a...at sites homologous to the locations they were attached to in MEK. The photoswitchable versions of MEK1...

...genes that help bacteria invade hosts, including attaching to host cells and evading the host immune system...resistance plasmids, which protect the host from attacks by other organisms. To conserve energy, these specialized...

...TAL2002 TGGTGCCCAT eGFP-TALEN-02-Right TAL2003 TTACGTCGCC eGFP-TALEN-03-Left TAL2004 TCTGCACCAC eGFP-TALEN...TGGTGCCCATCCTGG eGFP-TALEN-22-Right TAL2043 TTGTGGCCGTTTACG eGFP-TALEN-23-Left TAL2044 TGCCCGAAGGCTACG ...TGTTCACCGGGGTGGT eGFP-TALEN-26-Right TAL2051 TTACGTCGCCGTCCAG eGFP-TALEN-27-Left TAL2052 TGCCCATCCTGGTCGA...TCACCGGGGTGGTGCCCA eGFP-TALEN-36-Right TAL2095 TGGCCGTTTACGTCGCCG eGFP-TALEN-37-Left TAL2096 TAAACGGCCACAAGTTCA...TTCACCGGGGTGGTGCCCAT eGFP-TALEN-43-Right TAL2073 TTGTGGCCGTTTACGTCGCC eGFP-TALEN-44-Left TAL2074 TGCACCACCGGCAAGCTGCC...

...Despite these grim statistics, new strategies for attacking cancer are yielding promising results in the laboratory...universally-dividing behavior of cancer cells, attacking the cellular machinery necessary for division....

...covalently linked molecules (i.e. photoswitches) attached near the binding pocket of an engineered receptor...PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody that ...

...are small plastic containers that can easily be attached with a hose to a nitrogen gas tank with a pressure...encoded by the plasmids in the library will remain attached to the membrane at the same location that their...

...cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt fox1 (a2bp1l) OZ519 and OZ520 OPEN OPEN...aCGTTCACTCggtaaTGGGCCGACt Atg7 OZ557 and OZ558 OPEN OPEN tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559 and OZ560 OPEN OPEN gGCCATCAGCtcgcattGGAGAAGTAg...gCTCATCTCCACCGAGCTGGAGAAg atp2c1 OZ567 and OZ568 CODA CODA tTACCTCCACGATCTTGCTGATGGAg dio2 OZ569 and OZ570 CODA OPEN aAGCTGCTCCAAACTCTAAGGTGGTg...gTCCAGCAGCGGTGAGGATGAAGACg unc119b OZ581 and OZ582 CODA CODA tTGCTGCCGCGGATGCTGTGGCGGTTa cx52.9 OZ583 and OZ584 OPEN OPEN cTTCATCTTCCGCATGCTGGTGCTg...

...What’s my angle on attacking this problem?”, try thinking “Who can I assemble to attack this problem from...

... at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al. 2013). This approach misses larger mismatches...25595557. PubMed Central PMCID: PMC4308725. 7. Pattanayak, Vikram, et al. "High-throughput profiling of...

... analysis, you'll need to use fluorophores that attach to the target. When you design a flow experiment...cells with fluorophore-conjugated antibodies that attach to a specific target. By using a mix of different...

... before.  These attacks on science ultimately galvanized scientists to rally in Ottawa to protest the ...

...the nascent heteroduplex and abolish cleavage (Pattanayak et al., 2013). As mentioned above, however, Cas9...e96941. https://doi.org/10.15252/embj.201796941 Pattanayak, V., Lin, S., Guilinger, J. P., Ma, E., Doudna...

... outcome, since not all cell types are easily attainable, or easy to reprogram. Basically, there’s no ...Deepak M. Gupta, Mei Huang, Zongjin Li, Nicholas J. Panetta, Zhi Ying Chen, Robert C. Robbins, Mark A. Kay,...

...telomeres and found that TZAP binds directly to TTAGGG repeats via its zinc finger domains. This binding...binding was also independent of another TTAGGG binding complex, shelterin. Swapping the DNA binding domain...

...cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with 2µg/ml of Doxycycline...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor...

...integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed information about...promoter integrated in the chromosome at phage attachment sites. The levels of the two reporters, mCherry...

...detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody is done through...direct ELISAs, an antigen or protein of interest is attached to the well of a 96-well plate. A conjugated primary...recognize the protein in its native conformation. Attachment of the antibody to the beads is typically done...