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  1. Tetracycline Inducible Expression

    Type
    Collection
    ...promoter driving tTA; contains insulator sequence; plasmid pEnt L1L3 rtTA-3 is a rtTA version tTA Off Hsiao ...tetracycline-controlled transactivator (tTA) was developed. tTA was created by fusing tetR with the C-terminal..., which controls GFP expression tTA Off Verma 26803 pEnt L1L3 EF1a-tTA-2 Contains Gateway L1L3 sites and...34856 fos-tTA Mouse fos promoter plus part of the first intron (-764/+918) and fos 3'UTR tTA Off Mayford...transregulation. Dox binds with high affinity to tTA and rtTA; thus, dox can be used in both tetracycline ...interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR On or Off PI 21916 Tet-pLKO-neo 3rd generation...minimal CMV and is silent in absence of binding of tTA or rtTA None On Vogelstein 25735 pSLIK-Neo Lentiviral...

  2. AAV Molecular Tools

    Type
    Collection
    ...99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru ...-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a ...feedback loop for amplified tTA expression. 1 Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible... tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru ...

  3. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...non-standard), expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Gradinaru 103006 pUCmini-iCAP-PHP.S...non-standard), expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Gradinaru 127847 pUCmini-iCAP-PHP.V1...non-standard), expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10...non-standard), expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22...non-standard), expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1...), expressing Rep2 and MaCPNS1 Cap controlled by tTA-TRE amplification system Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2...), expressing Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Gradinaru RepCap Plasmids...

  4. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1 promoter that contains...amplifed expression of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent...-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed...amplifed expression of the tTA Viviana Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses...Gradinaru 100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected...to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP...

  5. AAV for Neuronal Tracing

    Type
    Collection
    ...AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant...

  6. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...apoa4-like TAL3023 & TAL3024 TGAAGGTTCTTGTGGTGCtcacacttgctgtggtTACAGGTAAGAAACTAAA apoeb TAL3402 & TAL3403 ... atg12 TAL3216 & TAL3217 TTAATCAGTCGTTTGCGCcatcaccggaccaagaAGTTGGTGTGCTTTTTGA avpl TAL3220 & TAL3221 TGCTGTGTGTGCTCGCGCtctccacgctctcgtcTGCCTGCTACATCCAGAA...TCAGCTCCCCGGAGAGGCaccgcactgctccagggTTGGATGATGGACATGGA bsx TAL3034 & TAL3035 TCTCAGACTCACAGTTATccggactcgagaaaagGTTCGAGATCCAGAGATA btr30 TAL3036 & TAL3037 ...TATAACAAATCCCACCACattgcccgatatcttgAATACTTCCAAAACAAAA Ccl2 TAL3222 & TAL3223 TTTTCGTTTTCCACTATAaagatcccagttaagcAGGTTCAGAGTTATCATA cebpa TAL3040 & TAL3041 ... cebpb TAL3224 & TAL3225 TGGAAGTGGCCGGTTTTTacgagggggactacctCGCTTTCCACAGCACTAA cebpd TAL3042 & TAL3043 ...TTCTCAAAATGAGAGGGGtgaatctgaagttcaaCAAACCCAGCAGCACCAA CIT1 TAL3226 & TAL3227 TGAAGTTTAAATATGGAGgtcatggcagtgttaaAGATCTGTCCGCAGTCGA cited2 TAL3228 & TAL3229...cited4a TAL3046 & TAL3047 TGAACCATGGTTCTGCTGgcggaggcctgcatggTTATCGCATGGGAATGAA cited4b TAL3048 & TAL3049...

  7. Validated gRNA Sequences

    Type
    Collection
    ...cerevisiae ATTGCGATCTCTTTAAAGGG 64333 cut S. pyogenes 25281382 Jin HPRT H. sapiens GACTGTAAGTGAATTACTT 69539...23287722 Church actII-orf4 S. coelicolor ATTACCAGGGACCGGAGTTC 62552 cut S. pyogenes 25739462 Jiang ade6...pyogenes 26472758 Sabatini ANT1 S. lycopersicum TGTCGTTTATAATTTGTAGA 70019 cut S. pyogenes 26541286 Voytas APC...pyogenes 25619936 Sato ASCL1 H. sapiens TGGTGTTTATTCAGCCGGGAGTC 64132 activate S. pyogenes 25619936 Sato...pyogenes 24967838 Mashimo ATF1 H. sapiens TAGGAATCAAACACTTTTATTGG 64690 tag S. pyogenes 26355004 Mendenhall...pyogenes 24954249 Yamamoto avr-14 C. elegans GATTGGAGAGTTAGACCACG 58981 cut S. pyogenes 24879462 Mello avr...pyogenes 24954249 Yamamoto CREB1 H. sapiens GCCACAAATCAGATTAATTTGG 64940 tag S. pyogenes 26355004 Mendenhall...

  8. TALENs for Endogenous Human Genes

    Type
    Collection
    ...) ABL1 TAL2380 & TAL2381 TACCTATTATTACTTTATggggcagcagcctggaaAAGTACTTGGGGACCAA AKT2 TAL2382 & TAL2383 TGTGTCTTGGGATGAGTGggtcagtgttctggtgCTCACAGGATGGCTGGCA...TATGTACGCCTCCCTGGGctcgggtccggtcgccCCTTTGCCCGCTTCTGTA ATM TAL2402 & TAL2403 TGAATTGGGATGCTGTTTttaggtattctattcaaaTTTATTTTACTGTCTTTA AXIN2 TAL2200 & TAL2201...BMPR1A TAL2260 & TAL2261 TACAATTGAACAATGCCTcagctatacatttacatCAGATTATTGGGAGCCTA BRCA1 TAL2384 & TAL2385 ... BRCA2 TAL2302 & TAL2303 TTAGACTTAGGTAAGTAAtgcaatatggtagactGGGGAGAACTACAAACTA CBX3 TAL2358 & TAL2359 TCTGCAATAAAAAATGGCctccaacaaaactacaTTGGTAAGTTAATGAAAA... TCTGCAATAAAAAATGGCctccaacaaaactacaTTGGTAAGTTAATGAAAA CBX8 TAL2360 & TAL2361 TGGAGCTTTCAGCGGTGGgggagcgggtgttcgcgGCCGAAGCCCTCCTGAA... CDC73 TAL2202 & TAL2203 TGCTTAGCGTCCTGCGACagtacaacatccagaaGAAGGAGATTGTGGTGAA CDK4 TAL2404 & TAL2405 TCCCTTGATCTGAGAAtggctacctctcgataTGAGCCAGTGGCTGAAA...CTNNB1 TAL2262 & TAL2263 TCCAGCGTGGACAATGGctactcaaggtttgtgTCATTAAATCTTTAGTTA CYLD TAL2386 & TAL2387 TAATATCACAATGAGTTCaggcttatggagccaagaAAAAGTCACTTCACCCTA...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...MATR3 Flag CMV ALS Yossi Shiloh 34927 pTRE_tau-LacZ::tTA-H100Y MAPT LacZ TRE Parkinson's, FTD Mark Mayford...HNRNPA1 His T7 ALS Tanja Mittag 169714 A1-LCD deltahexa HNRNPA1 His T7 ALS Tanja Mittag 169715 A1-LCD Aro- ...HNRNPA1 His T7 ALS Tanja Mittag 169716 A1-LCD Aro-- HNRNPA1 His T7 ALS Tanja Mittag 169717 A1-LCD Aro+ HNRNPA1...HNRNPA1 His T7 ALS Tanja Mittag 169718 A1-LCD Perfect Mix HNRNPA1 His T7 ALS Tanja Mittag 169719 A1-LCD Patchy...HNRNPA1 His T7 ALS Tanja Mittag 178659 A1-LCD-12F+12Y HNRNPA1 His T7 ALS Tanja Mittag 178660 A1-LCD+7F-7Y ...HNRNPA1 His T7 ALS Tanja Mittag 178661 A1-LCD-9F+6Y HNRNPA1 His T7 ALS Tanja Mittag 178662 A1-LCD-8F+4Y HNRNPA1...HNRNPA1 His T7 ALS Tanja Mittag 178663 A1-LCD-9F+3Y HNRNPA1 His T7 ALS Tanja Mittag 178664 A1-LCD-10R HNRNPA1...

  10. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Pair C5V Cerulean attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus via a 17...linker C32V Cerulean attached to Venus via a 32 amino acid linker C5A Cerulean attached to Amber via a 5 ...linker C17A Cerulean attached to Amber via a 17 amino acid linker C32A Cerulean attached to Amber via a 32...amino acid linker mGFP-10-sREACh-N3 Monomeric EGFP attached to super-REACh via a 10 amino acid linker ACA ...

  11. TALENs for the EGFP Reporter Gene

    Type
    Collection
    ...TAL2002 TGGTGCCCAT eGFP-TALEN-02-Right TAL2003 TTACGTCGCC eGFP-TALEN-03-Left TAL2004 TCTGCACCAC eGFP-TALEN...TGGTGCCCATCCTGG eGFP-TALEN-22-Right TAL2043 TTGTGGCCGTTTACG eGFP-TALEN-23-Left TAL2044 TGCCCGAAGGCTACG ...TGTTCACCGGGGTGGT eGFP-TALEN-26-Right TAL2051 TTACGTCGCCGTCCAG eGFP-TALEN-27-Left TAL2052 TGCCCATCCTGGTCGA...TCACCGGGGTGGTGCCCA eGFP-TALEN-36-Right TAL2095 TGGCCGTTTACGTCGCCG eGFP-TALEN-37-Left TAL2096 TAAACGGCCACAAGTTCA...TTCACCGGGGTGGTGCCCAT eGFP-TALEN-43-Right TAL2073 TTGTGGCCGTTTACGTCGCC eGFP-TALEN-44-Left TAL2074 TGCACCACCGGCAAGCTGCC...

  12. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt fox1 (a2bp1l) OZ519 and OZ520 OPEN OPEN...aCGTTCACTCggtaaTGGGCCGACt Atg7 OZ557 and OZ558 OPEN OPEN tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559 and OZ560 OPEN OPEN gGCCATCAGCtcgcattGGAGAAGTAg...gCTCATCTCCACCGAGCTGGAGAAg atp2c1 OZ567 and OZ568 CODA CODA tTACCTCCACGATCTTGCTGATGGAg dio2 OZ569 and OZ570 CODA OPEN aAGCTGCTCCAAACTCTAAGGTGGTg...gTCCAGCAGCGGTGAGGATGAAGACg unc119b OZ581 and OZ582 CODA CODA tTGCTGCCGCGGATGCTGTGGCGGTTa cx52.9 OZ583 and OZ584 OPEN OPEN cTTCATCTTCCGCATGCTGGTGCTg...

  13. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed information about...promoter integrated in the chromosome at phage attachment sites. The levels of the two reporters, mCherry...

  14. Antibody Guide

    Type
    Collection
    ...detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody is done through...direct ELISAs, an antigen or protein of interest is attached to the well of a 96-well plate. A conjugated primary...recognize the protein in its native conformation. Attachment of the antibody to the beads is typically done...

  15. CRISPR Guide

    Type
    Collection
    ..., 481–485. PMID: 26098369 Kleinstiver, B. P., Pattanayak, V., Prew, M. S., Tsai, S. Q., Nguyen, N. T.,...PMID: 25398340 Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained...Daringer, N. M., Freije, C. A., Myhrvold, C., Bhattacharyya, R. P., Livny, J., Regev, A., Koonin, E. V.,... Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T. (2019). CRISPR-Cas3...

  16. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...60601 Mammalian BamHI/EcoRI none S. pyogenes Hygro Hotta pUC-H1-gRNA 61089 Mammalian BsaI none S. pyogenes.../110) 71707 Mammalian hU6 yes, cut S. pyogenes Draetta pCAS1yl 73226 Other/Yarrowia lipolytica yes, cut...

  18. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...amplified with the following primers: FW 5'–GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid...

  19. Retrovirus Plasmids

    Type
    Collection
    ...MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional plasmids in...
Showing: 1 - 20 of 30 results