...and the promoter of the 5’LTR has been deleted to reduce its activity. A CMV or an EF1α promoter has been...the control of two different promoters. Both the single and dual promoter transfer vectors can be used...containing the rev response element (RRE), an internal promoter, and the desired gene (transfer vector plasmid...entry site (IRES) and expressed under the same promoter. Alternatively, your gene and the marker can be...cell cycle, a transfer vector with an inducible promoter (see below) should be used. Inducible lentiviral...Tet-response element (TRE) is placed upstream of the promoter in your transfer vector. In the absence of tetracycline...

...experiment.  Promoters commonly used to control expression of Red include the IPTG-inducible lac promoter, the...the arabinose-inducible pBAD promoter and the endogenous phage pL promoter.  It is a good idea to choose...Beta: Beta protects the ssDNA created by Exo and promotes its annealing to a complementary ssDNA target ...gam is tightly regulated by the endogenous phage promoter pL and repressor CI. For recombineering purposes... express the repressors associated with these promoters (lacI, araC, cI857) to limit leaky expression ...into the genome.  Mini-λ uses the endogenous red promoter pL and the cI857 repressor to regulate expression...

... to give scientists access to a wide array of promoters and fluorophores for validating and testing purposes... pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE...-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs ubiquitous expression. pAAV-mDlx-NLS-mRuby2 (99130...-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter directs strong, ubiquitous expression. This is...

...TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence and ultimately...studying the effects of endogenous looping on distal promoters and enhancers, or have attempted to engineer loops...LADL Anchors to genomic targets, such as a distal promoter and an enhancer. What makes LADL unique from previous...group targeted the enhancer region of Klf4 and the promoter region of Zfp462, which are over 500kB apart, ...

...tandem with a single promoter, but only the gene directly following the promoter is expressed. Cre-mediated... As RFP and OFP are directly downstream of the promoter in Brainbow 1.0 and 1.1, respectively, cells will...Brainbow system are that it does not use ubiquitous promoters, meaning that not all cell types can be labeled...placing Brainbow downstream of a neural-specific promoter, or the entire fish could be labeled by injecting...an RFP, CFP, and YFP downstream of a ubiquitous promoter. Zebrabow is an extremely versatile technology...

...start of the gene to end up just downstream of the promoter found in the backbone. Ideally, the backbone will...cut sites (restriction sites) downstream of the promoter as part of a multiple cloning site (MCS). Having...easily orient your gene insert with respect to the promoter. For example, let’s say your plasmid backbone ... on the left side of the image below. It has a promoter (blue arrow) followed by the restriction sites...gene in the proper orientation downstream of the promoter, you can add an EcoRI site just 5’ of the start...the 5’ end of your gene just downstream of the promoter and placing the gene in the proper orientation...

...Gal4 expression can be regulated by any chosen promoter, and Bloomington’s Stock Center has fly lines ...lines expressing Gal4 under a variety of promoters resulting in tissue specific expression. To express YFG...interest directly downstream of a tissue-specific promoter sounds simpler, this modular system saves you ... to making a new fly with hedgehog under a new promoter each time a new hypothesis is tested. Controlling...

...vitro models for lung disease. Darrell strives to promote open source biology in his own lab, at the Center...to help keep us focused on our core mission to promote scientific sharing. Listen to learn more about ... defines Open Science and how he practices and promotes open science in the lab - sharing reagents, cell...

...approximately 19 kb in length. It contains short promoter sequences at its 3’ and 5’ ends that are crucial..., the roles of specific viral sequences (e.g. promoters) can be studied by changing the minigenome cis-acting...minigenome) are under control of a T7 RNA polymerase promoter sequence. Thus, T7 RNA polymerase also needs to...leader region acts as a functional BRSV genome promoter." Journal of virology 73.1 (1999): 251-259. PubMed...Michael, et al. "The Ebola virus genomic replication promoter is bipartite and follows the rule of six." Journal...

...bidirectional promoters, or the creation of bicistronic or multicistronic vectors. Unlike promoters which will... proteins from the same mRNA. We've discussed promoters before so in this blog post we’ll cover basics...screenable or selectable markers from a unique promoter, multicistronic plasmids ensure that any cells...originally discovered in poliovirus RNA, where it promotes translation of the viral genome in eukaryotic ...

...) Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...viscosity of PEG. Stirring under medium heat will promote faster dissolution. Adjust the pH to ~7.4. Autoclave...

...screening of human promoters, the Zhang Lab found a short fragment of human IBA1 promoter (hIBA1a, 466 bp...small RNA-binding exonuclease protection factor) promotes editing (Yan, J et al., 2024). They developed ... expression from ssAAV5s with hIBA1 or hIBA1a promoters in mice. B) Quantifications showing high microglia...a CC-BY-NC-ND license.  Integrating hIBA1a promoter into AAV vectors, either alone or in combination...

...technique is especially useful for introducing promoters, terminators, and other short sequences into the...

...overcome by fusing opsin genes to cell-type specific promoters; however, those usually drive weak expression ...expression of downstream genes. Strong cell-specific promoters, on the other side, are too long for rAAVs, which...2018). Optogenetic FLEx vectors contain a strong promoter upstream of an opsin gene (e.g., channelrhodopsin... the antisense orientation with respect to the promoter to prevent its expression, and is flanked (“floxed...rAAVs expressing Cre under a specific cell-type promoter. Once injected into the brain, the viruses infect...thereby enabling its expression under a strong promoter only in specific neuronal cells. FLEx ensures,...

...Electroporation is one popular method scientists use to promote transformation, but it isn’t just for lab specimens...specimens — there is evidence that lightning can also promote gene transfer in soil bacteria! (Demanèche et al...chromosome, but when encoded on plasmids, they can promote rapid spreading of genetic material through a population... features, such as origins of replication and promoters, check out our other Plasmids 101 posts, and keep...

...the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter, and rabbit... tags and fusion proteins Finally, we confirm promoters, tags, fusion proteins, and selectable markers...

...Effective science communication is required to promote public support for research and to keep useful ... a graduate student organization that works to promote science communication. Science in the News hosts...

... gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene’s fly library web page... over 30 promoters and 140 genes, including constructs with unique tissue-specific promoters expressing...genomic libraries are cloned downstream of a minimal promoter sequence. Should one of these random stretches...activate its own transcription from the minimal promoter and its strength can be determined from its enrichment...variety of plasmids containing many different promoters, RBS’, coding sequences (CDS), and terminators...fluorophores, generate expression vectors with various promoters suitable for in vivo expression, and/or produce...continuous expansion of compatible backbones, promoters, and genes available to the community. If you ...

... a tool that can help you advance your career, promote career growth, and/or switch your field of work...is larger and there are more opportunities for promotion. It’s not uncommon for bachelor’s and master’s..., i.e. Principal Scientist or Scientist I/II. Promotions don’t happen overnight though, and it might take...

...the MCS is often downstream from a promoter. Insert Gene, promoter or other DNA fragment cloned into ...into the MCS for further study. Promoter Region Drives transcription of the target gene. Vital component...