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Showing: 41 - 60 of 125 results

  1. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...genome engineering tool offers flexibility, multiplexibility, and ease of use. In order for this technology...the tool’s specificity, orthogonality, and multiplexibility in various species and describe an assortment...

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...diversity introduced by lambda red recombineering and Multiplex Automated Genome Engineering (MAGE) will always...progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR to target more than ...

  4. Depositor Collections

    Type
    Collection
    ...FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection...

  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...genomic loci at once (get more information on multiplexing gRNAs here). Cas9 is commonly tagged with a ...Zhang Y, Qi Y (2015) A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation...Resources on the Addgene Blog Read Up on CRISPR Multiplexing Tools Validate Your Genome Edit Design Your ...

  8. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...

  9. Antibodies 101: Conjugation

    Type
    Blog Post
    ... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...

  10. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ... 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate cloning...Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression of gRNAs Choosing a Molecular Cloning...

  11. The Twelve Days of CRISPR

    Type
    Blog Post
    ...the twelfth day of CRISPR, Addgene gave to me: multiplexed gRNAs.  We hope you’ve been enjoying our CRISPR...

  12. Deisseroth INTRSECT Collection

    Type
    Collection
    ...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to...definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations...

  13. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...counts making them fast and viable options for multiplex sequencing in many laboratories. Nevertheless,...DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic...dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to...

  14. CRISPR Library Amplification

    Type
    Protocol
    ...screening, barcoding, or other high throughput multiplexed experiments. These pooled plasmids differ by ...

  15. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...detection (14-20). LDR has also been extended to multiplexed probe sets that allow the simultaneous interrogation...extends earlier studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used...: PMC148763. 15. Pingle, Maneesh R., et al. "Multiplexed identification of blood-borne bacterial pathogens...

  18. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...non-deletion and deletion bands ( Figure 2 ). Consider multiplexing the “deletion” and “non-deletion” PCR primer...primer pairs in a single reaction. Optimize multiplexing in a polyclonal population ( i.e., bulk sorted cells...amplicons be easily resolved on an agarose gel for multiplexing. Screening CRISPR/Cas9 Clones for Deletions ...

  19. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...mitochondria Mitycam, or ER targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for...Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities. Nat Cell ...GFP-based fluorescent reporter cAMPr Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling...Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for...mitochondrial sensor for H 2 O 2 oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for...RdLight1 An expanded palette of dopamine sensors for multiplex imaging in vivo. Nat Methods. 2020 Nov;17(11):...redox sensors for cytosol, ER or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for...

  20. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...
Showing: 41 - 60 of 125 results