...correct integrated provirus using PCR. This assay involves transducing cells with serial dilutions of the...

... 1mm thick, 10-well, Invitrogen NP0321BOX 20X MOPS SDS running buffer, Invitrogen NP0001 4X NuPage sample...buffer, Invitrogen NP0007 10X NuPage sample reducing agent (500 mM dithiothreitol, DTT), Invitrogen NP0009...NP0009 PageRuler Plus Prestained protein ladder, Invitrogen 26619 Pipette tips, 10 µL, VWR 76322-132 Pipette...MOPS buffer into 475 mL of deionized water. Gently invert to mix. Procedure Section 1: SDS-PAGE Add 5 µL ...

...chemogenetic resources available at Addgene. See our inventory of chemogenetic constructs, including DREADD constructs...

...Gateway vectors) Zeocin® is an InvivoGen trademark. Back to Top Viral Expression and Packaging...

...which can result in unintended system induction. Investigate whether or not your serum is tetracycline-free...

...Currently supports over 20 model and non-model invertebrate species. Forums and FAQs Feng Zhang lab: Tips...

...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Please...

...serum-free media Invitrogen: #31985 Dulbecco’s Modified Eagle Medium (DMEM) Invitrogen: #11995 Fetal Bovine...Bovine Serum (FBS) Invitrogen: #16000 Penicillin/Streptomycin Invitrogen: #15140-122 Polypropylene tubes... buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen...homologous recombination of unstable regions, such as Invitrogen Stbl3™ or NEB Stable cells. This will ensure ...

...frequency and exact sequence of targeted alleles. Involves PCR amplification of targeted region from DNA,...

...CRISPRs into Cells of Interest NOTE: This protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation...

...of interest. pJT106b3 31393 Red light inducible inverter Red light (650 nm) Christopher Voigt When combined...

...Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters...

...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Please...

...Addgene's viral vectors See Addgene's Current AAV inventory , which includes all viral vectors transferred...

...incubator Pipet controller Hazardous waste container Inverted fluorescence microscope Reagents DMEM high glucose...mL of DMEM complete. Mix well by pipetting or inverting. Aliquot 2 mL of cell suspension into each well...

...fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with the...

...Knockout Human L. Liu 3rd 5 10,000 Zeocin® is an InvivoGen trademark....

...your application. A diagnostic digest typically involves ∼500 ng of DNA, while molecular cloning often ...s) following the digestion reaction. This may involve incubating the reaction at 70 °C for 15 mins, or...

... thoroughly by inversion. Add 1 mL water-saturated phenol, mix thoroughly by inversion. Add 0.2 mL of ...

...spreading of antibiotic makes it easy for an investigator to conveniently plate and select transformed...