...also like... Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Image is released to public...

...Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA...for use in zebrafish Guide RNA expression plasmids for the EGFP Reporter Gene Guide RNA expression plasmids...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome... gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type ...RGNs consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’...web-based ZiFiT Targeter software to enable identification of potential RGN target sites and to facilitate...facilitate design of short oligonucleotides needed to construct gRNA expression vectors. More information ...

... 24 bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled... has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled...

...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning...to faithfully introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication Empty...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Vector Type Promoter PI Publication...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

...bacteria. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs is not considered "scarless...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

...ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter..."Cdc42", "transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter...Optogenetics Guide AAV Viral Service Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent ...Fluorescent Protein Guide Microbial Opsin Plasmids Optical Switch Plasmids Background Optogenetics plasmids encode...opsins to be adopted as an optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas...Variants of channelrhodopsin have been engineered or identified to increase the spectral and kinetic properties...in these tables, refer to Addgene's Optogenetics Guide . Interested in more news about optogenetics research...

...efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry...target the solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561...transporters in the human genome with approximately 450 identified members. Many SLCs have mutations associated ...proteomic interactions represent the framework that provides biological context. Assays monitoring impedance...

...structure, and interactions! Plasmid ID Promoter Epitope Tag(s) ID Purpose pDEST-HisMBP 11085 Tac (lactose...harness bacterial genomes for your research. Plasmid ID Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini...of interest does when expressed in vivo . Plasmid ID Visualization Technique/Tag PI Purpose Empty Backbones...systems with your gene/protein of interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids...activate expression of the reporter gene. Plasmid ID Promoter Reporter PI Purpose pRU Series Various Plasmids...localization to aid disulfide bond formation F Xavier Gomis-Ruth Kit containing plasmids with a wide variety of... like… Plasmids 101 Addgene's Molecular Biology Guides Plasmids 101: E. coli strains for protein expression...

...GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression... the TEF1 or PGK1 promoters and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication...

...plasmids available to industry is continually growing. ID Plasmid Description Industry PI Return to top Other...Coronavirus Plasmids: SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control...SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry PI For more information...helpful for COVID-19 research in the table below: ID Plasmid Description Industry PI Do you have a suggestion...Ectodomain and RBD mutants that can be used to identify antibody escape mutants and for comprehensive ... or Inserts Several mammalian genes have been identified as having a key role in coronavirus infection...and related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK and...

...cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6...Serotypes Available as a Viral Service at Addgene ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1...Serotypes Not Available as a Viral Service at Addgene ID Plasmid Capsid Description PI 64839 7M8 7M8 AAV packaging...based on plasmid name, species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids 101-Viral... virus. Instead, specific gene products can be provided by helper plasmids (e.g., pHelper) and specific...HEK293 cells) during AAV production. Read our AAV Guide for more information about: AAV components Common...

...that are currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description...as well as by our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications...intellectual property rights, and provides liability protection for material providers. Addgene’s deposit agreements...Kit for using comprehensive end-joining ligation fidelity and bias data to predict high accuracy junction...

...Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description PI 8449 pUMVC Packaging ...for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA...Plasmids Additional Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors...insertional mutagenesis). Read our γ-Retrovirus Guide for more information about: γ-Retroviral packaging...

...plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GTPase Arf1...plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GAP Arfgap1... is regulated both upstream (by GEFs, guanine nucleotide exchange factors), and downstream (by GAPs, GTPase...

...used in worms, search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing...C. elegans, C. briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed...Caenorhabditis elegans ( C. elegans or “the worm”) is widely used as a genetic model organism due to their rapid...community that aren't in the repository? Help us widen our resources for the community by depositing your...ordered individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology....elegans. Silencing Genomes - Cold Spring Harbor's guide to RNAi in C. elegans WormAtlas - A database featuring...

... antibodies and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species...

...such as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Return...as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter PI Return to top Other...like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription.... The tables below highlight plasmids spanning a wide variety of molecular biology technologies: from ...Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology ...

...endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP...May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization...Institute for Cell Science has several protocol videos available that may be helpful for working with ...

...Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP...will instead form white colonies. Adenoviral Vector ID Purpose pAdx-CMV-LacZ 73345 Expresses lacZ from the...Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and Resources Addgene...Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors Kazuhiro... of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for packaging...