...more attractive candidate for that ideal job. How can you identify your transferable skills? PLoS One ...collaboration and fail to identify it as a personal strength until it is identified by another person. Similar...when a new venture presents itself even outside of work, consider challenging yourself, take the opportunity...All while still paying bills and having a life outside my job. The same can be said about teamwork, communication...transferable skills so important? It is important to identify which transferable skills you possess because ... and making connections, avoid falling into an identity trap where you assume your technical skills define...wants to hear a specific time that you thought outside the box to solve a problem. When they ask about...

...high fidelity ligase—and when is an alternative ligase a better choice? And what is ligase fidelity anyway...are "higher fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase...annealed complementary regions. Why use a high fidelity ligase? Numerous applications have been developed...developed to take advantage of the high fidelity of Taq and other thermostable DNA ligases. These applications...applications detect specific nucleotide sequences with high specificity and quantitative accuracy and even include... include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase Detection...PCR amplified fragment) and treated with a high fidelity thermostable DNA ligase. If the target sequence...

... contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene...within the Addgene catalog. These IDs allow you to unmistakably identify a plasmid available through Addgene...VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier format for lenti-Cas9-VQR is...will be assigned an Addgene ID #, RRID, and resolvable compact identifier. Addgene works hard to ensure...including plasmids. All plasmids at Addgene have unique ID numbers associated with them, which serve as unique...about each plasmid item. Given a specific Addgene ID # listed in a publication, you can quickly pull up...the “Articles Citing this Plasmid” section. Having IDs linked to these metadata and publications help to...

...is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how to use...incredible new CRISPR technologies developed and widely adopted in the past few years, as well as refreshing...refreshing the existing content. Access the CRISPR Guide here! Highlights include a much-anticipated prime... PRIME editing Please check out the updated guide for a great introduction to what CRISPR technologies...

... viral attachment and fusion. Once inside a host cell, new evidence in PLoS Pathogens shows that the Ebola...week within two months. That said, Ebola is not considered a “terribly infectious disease” – infection occurs...via body fluids not through the air and that’s considered unlikely to change – but the illness has been...thought we’d do a little digging and assemble a rough guide to the science of Ebola and related resources. Ebola...

...alone. So, if you consider yourself part of the scientific community, get used to the idea of working on ...our kit process substantially and have plenty of ideas for further improvements. Types of teams There ...the first few meetings and we’ve volunteered our ideas, skills, and time to the cause ever since. Unlike... necessarily need a diverse group of people to provide that base. Sometimes there’s just too much work...ever taken has declared me an introvert, and I identify as one.  But some time around 10th grade, I snapped... a happier person. What does a good team need? Besides that all-important sense of purpose, there are ...multiple people: someone to coordinate, someone to decide, someone to delegate, etc. If you find that there...

... and adenoviruses in this easy-to-use guide. Print out the guide and use it for quick reference when you're...information on viral vectors? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service...pages. Click on the Image Above to Download the Guide ...

...three or four guides directed towards that gene as there can be a large difference in guide potency. Thus...whichever guides are closest to the transcription start site (TSS). We recommend targeting the guides to a ...scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there has...required for Cas9-based activation. CRISPRa for genome-wide studies One of the best uses for Cas9 activators...  Which Cas9 activator should I use? There are a wide variety of activators you can use for your experiments...transcription. That being said, we generally pick guides by putting the promoter of the gene into a gRNA...(2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome Biol 16:...

...share ideas. Ideas had over lunch are just as valuable! In 2019, Addgene implemented a company-wide New ...Transferable Skills Guide: Problem-Solving Transferable Skills Guide: Teamwork Transferable Skills Guide: Identifying...are still struggling with idea generation, try this thought exercise: Consider the issue as if there were...faster that way— but the idea behind the study holds. To generate novel ideas, start with an out-of-the-box...most prominent idea, and instead building an environment where everyone has their ideas heard.  So how...in which the goal is to generate unique ideas. Recognize ideas can come any time. Meetings shouldn’t be...to encourage the sharing of ideas, not to give people more work. Identify your enemies In Felicia Day...

....    If this video is helpful, you can find our complete collection of protocol videos on the Addgene...antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies...

...then, in having a guide that curates this information. And so I created the Codon Guide to Synthetic Biology...The video channels are also underrated and I recommend checking them out, especially since videos on synthetic...knowledge but little biology experience.) The Codon Guide to Synthetic Biology One of the best ways to learn...begin their journey into synthetic biology. The guide is a curated list of research papers, blogs, free...send me suggestions. The most useful parts of the Guide are probably the papers and textbooks. I’ve selected...to find. I hope that many people will find this guide useful as they start their synthetic biology journey...

...backgrounds. Sometimes this can lead to wildly different ideas of how to approach a problem, but it is these very...Addgene. Another advantage of Scrum is that it provides transparency to everyone involved about the status...serve Addgene and ourselves. We are also able to consider different technical approaches that may come up...from the user's perspective which in turn helps us decide how to best approach it in our code.  Sometimes...our knowledge, and love to collaborate, which, incidentally, are the same principles for open science. I...

...Today in our transferable skills guide: Collaborating with others outside your organization also known as...communicate outside my scientific expertise. Organizing a career fair for early-career scientists provided a crash...Interestingly, the ability to work with others outside one’s organization was found in the aforementioned...important in a non-research context, especially when considering small to mid-sized companies and organizations... Addgene as a nonprofit organization is very considerate and thoughtful about its limited resources. Rather... curve for collaborating and it is always a good idea to start close to your comfort zone and slowly expand...Collaboration opportunities within an organization but outside your comfort zone As part of my position at Addgene...

... The Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video to Share Your... we’ve gained along the way to ourselves so we decided to share some of our tips in our new series: Addgene...Have some suggestions for future tips?  Send your ideas to [email protected].  Additional Resources on the...Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol Pages Find Molecular...

... that one on one discussion can be confidential. Finally, provide timely feedback. We often associate...need some help. Today in our transferable skills guide: managing a team. You’ve just been promoted, congratulations...advance as much as possible so I could more easily identify what to delegate and to whom (make sure that person...expectations, answer any questions, and (most importantly) decide on a timeline. My experience is that tasks often...of X’s work for a couple of weeks now”. I had no idea this was happening even though I met with X on a...when having a mentor - someone more experienced to guide you - is valuable. Protect your team Make you team...impact your work and your life. It’s up to you to decide which one you want to be!  References Becoming...

...need some help. Today in our transferable skills guide: Time Mangement for Scientists. Back when I used...my own experiments in graduate school, I had to divide my time between lab work and practicing my SciComm... but if you ever find yourself in need of a few ideas, I hope you’ll find this post useful. After a bit...which tasks to actually do, you should err on the side of things that have the most utility; i.e. those...according to my priorities, I have a pretty good idea about the specific things I’ll be doing through ... it’s enticements are far too distracting. (As a side note, I’d recommend the book, “Bored and Brilliant...

...investigator who is using an in vivo animal model should consider the possible elicited immune response from viral...AAV. While these benefits make AAVs useful for a wide variety of experiments, care still needs to be taken...particular AAVs to use for your research. Below I’ll provide some tips that will help you think about how to...from https://www.addgene.org/viral-vectors/aav/aav-guide. Where in the tissue/what cell type? In the CNS,...distance traveled, etc.) (4). This has also been identified with AAV2 (5), AAV6 (6) and AAV5 at excitatory... many experiments, a group of researchers has identified this feature of AAVs as a new tool for neuroanatomical...miRNAs), short hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger nucleases, and many others...

...vectors Although retroviruses are still in use, considerable efforts have been devoted to develop efficient... Inducible lentiviral transfer vectors The most widely used inducible lentiviral vector system is the ...manipulated with care. See the NIH’s biosafety guidelines for more information and always be sure to consult...another. For experimental consistency it’s a good idea to purify and titer your virus. Virus purification.... Don’t hesitate to check our lentiviral vector guide to get more information and to get access to additional...Order Ready-to-Use Lentivirus Browse Our Lentivirus Guide Pages  ...

...comprehensive guide to Conflict Resolution. There are countless classes and resources that can provide details...ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork  ...” in quotations here because I would not have considered myself neutral in any of their disagreements....open new career opportunities for you, if you can identify them in yourself and learn how to sell them. Types...decision making, and stalled projects. You want to identify and address these types of harmful conflict early...other parties, the first step I always take is to identify the real source of the conflict. It can be easy...important to retain the stories and examples that have guided your resolution processes in the past. Types of...

...what I had done in order to identify potential sources of error and then decide on a strategy to fix the ...from the transferable skills guide: Teamwork Collaborating with others outside of your organization Time...need some help. Today in our transferable skills guide: Problem-Solving. I don’t know about your experience...went wrong with a PCR is a pretty niche skill; outside of a lab, where is that going to come in handy?...has been invaluable in my more recent positions outside of the lab. Granted, I currently work on the Scientific...situation, come up with potential solutions, and decide on a course of action that effectively resolves...solving as a generalizable process.  Step One: Identify the problem and gather information to help you...