...Xenopus. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...Publication C. elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/...Promoter Selectable Marker PI Publication Parasites ID Plasmid Gene/Insert Promoter Selectable Marker PI...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...efficiency than NHEJ. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...

... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI...diverse populations of mutants for directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI... and are potentially heritable in dividing cells. ID Plasmid Gene/Insert Selectable Marker PI Publication...

...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter...Publication C. elegans ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...your specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse... A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication RNA Targeting...

...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...homology-directed repair (HDR). Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

...material page (linked for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 37825-AAV1.T 20 ...material page (linked for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 50465-AAV1.T 20 ...please see our Control AAV Inventory . The catalog ID for the 20 µL sized testing sample is denoted with...with a .T suffix (for example, AAV1.T). The catalog ID for the 100 µL sized sample does not have the suffix...

...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI... A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable Marker PI...

...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog... or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering...your specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

...zebrafish. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Promoter Selectable Marker PI Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been made by...cytidine deaminase (AID). Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...

...which can be used to control gene expression. Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV...phSyn1(S)-DreO-bGHpA Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV...183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638...pAAV-EF1a-vCre EF1a none 8, rg* Deisseroth Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551...AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...

...protein can be paired with a wide variety of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication... A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...

...toolset for scientists worldwide. Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody...Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity... epitope tags and other common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity...used to create or express plasmid-based antibodies. ID Plasmid Description...the alpha subunit, outside the ligand-binding pocket Bind to the beta subunit, outside the ligand-binding...ligand-binding pocket Bind specific subunit pairs, outside the ligand-binding pocket Bind specific subunit pairs,...

...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick ...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog... base-to-base conversions using a prime editing guide RNA (pegRNA). The pegRNA directs the nickase to ...

...tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118... purification (which can be used for viral TRAP). ID Name Expression System Activity Serotype PI 98747...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509... encode tools that can be used for cell ablation. ID Name Expression System Activity Serotype PI 124364...

...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert ...Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

...: Table 1. dCas9 Activators sgRNA Dual Expression ID Plasmid 48236 pAC2-dual-dCas9VP48-sgExpression : ...Table 2. pmax dCas9 Activator Expression Plasmids ID Plasmid 48223 pAC91-pmax-dCas9VP64 : dCas9VP64 on...expression vecor. Table 3. dCas9 Activators Gateway Donors ID Plasmid 48214 pAC1-pCR8-dCas9VP48 : dCas9VP48 on ...pCR8/GW/TOPO. Table 4. Gateway Destination Vector ID Plasmid 48222 pAC90-pmax-DEST : Gateway Destination... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-on is an RNA-guided transcriptional...activator protein and the single guide RNA (sgRNA). While the dCas9 protein provides the transactivation activity...activity, the sgRNA component provides the specificity and is designed to target promoters or enhancers of...

...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene...whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...deletions, and point mutations using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA...

... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection...This method uses peptide identification by proteomics (Capture MS) to validate the identity of proteins recognized...first identify your assay type and what species your protein of interest comes from. Then consider the ...In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and...Collection Jump To: Antibody Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents...Antibodies can be used in the lab to quantify, identify, separate, and locate proteins of interest. The... between antibodies, allows for the antibody’s identity to be easily confirmed via sequencing, and enables...

...Science Guides CRISPR Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large...making CRISPR perfect for genome-wide screens. This guide will provide a basic understanding of CRISPR ...genomic target of the gRNA can be any ~20 nucleotide sequence, provided it meets two conditions: The sequence...discuss more Cas9 fusion tools later in the guide. Increasing fidelity of Cas9 Researchers have also enhanced...Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes...with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes...some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) ...

... TALENs TALEN Guide Addgene's Guide to TALEN Technologies...Scientists have identified a large number of zinc fingers that recognize various nucleotide triplets. With...Guide to TALEN technology and using TALEN plasmids TALENs...disciplines are coming closer to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays ...without their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and ...Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors, which ...