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Showing: 41 - 60 of 795 results

  1. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...cells. The problem is that the only version of full-length cDNA you can find for YGOI is in a bacterial expression...enzyme buffer (see (Link opens in a new window) New England Biolabs for more information about restriction ...

  4. Hot Plasmids - January 2023

    Type
    Blog Post
    ...within tumours via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021...antibody?  Similarities:  Generated against full length GFP and converted to recombinant form by the NeuroMab...these processes. The Liu and Petsko labs recently engineered hyperfolder YFP (hfYFP), a FP well-suited for...

  6. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ... Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018)...empower the scientific community with the tools to engineer them. Given the ease with which scientists can... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched...Monoclonal Antibodies for Clinical Use. In: Antibody Engineering. InTech Additional resources on the Addgene blog...

  10. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...their applications in neuroscience. Curr Opin Biomed Eng. 2019. 12:111-117. doi: 10.1016/j.cobme.2019.10.010... as bright as the other GEVI systems that use engineered FPs. Attempts have been made to optimize their...their fluorescence, which ultimately led to the engineering of the second type of these GEVIs: a FP-fused... voltage sensor. FP-rhodopsin GEVIs have been engineered to have kinetics comparable to its one component... transiently and for genomic integration. Lab-engineered viruses will package your genetic cargo in lieu...

  15. AAV Production in HEK293 Cells

    Type
    Protocol
    ... until fully dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under...

  16. CRISPR Library Amplification

    Type
    Protocol
    ...Quality Control (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but...

  17. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...of mice via adeno-associated viruses. Nat Biomed Eng 4:97–110 . https://doi.org/10.1038/s41551-019-0501...gRNA together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb... little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple...enough to package into AAV. At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the...ADAR2DD(delta984-1090) are approximately 4.1 kb in length, allowing them to be packaged in AAV.   Split ...transgenes might seem daunting, it’s actually a challenge that the field has overcome with split AAVs. In...When a cell is transduced by both AAVs, the full length gene and/or protein is reconstituted. One way to...

  18. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...enabling technologies in synthetic biology. J. Biol. Eng. 2013; 7, 13. PubMed PMID: 23663447. PubMed Central...fragments of the chromosome, which was technically challenging. Other homology based technologies We've described...Central PMCID: PMC3874208 Other Resources: New England Biolabs Gibson Assembly Overview Additional Resources...Protocols Gibson Assembly® is licensed to New England Biolabs by Synthetic Genomics, Inc. ...

  19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...mechanobiology: seeing is believing." Annu. Rev. Biomed. Eng. 10 (2008): 1-38. PubMed PMID:18647110. Additional...spectral properties upon exposure to a specific wavelength of light. PA-FPs can be activated from low fluorescent...brighter fluorescent state by exposure to a specific wavelength of light. The switch or photoconversion usually...multiple times by activating with two distinct wavelengths of light (see Table 2). The best known reversible...photoactivation) (6). Dronpa is photoactivated by blue wavelengths of light, causing emission of green fluorescence...another group of reversible PA-FPs. These were engineered to be the first reversible, monomeric red fluorescent...dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial matrix protein...

  20. CRISPR Guide

    Type
    Collection
    ...popular genome engineering approach. Figure 1: Overview of the basic CRISPR mechanism Engineered CRISPR systems...modified versions engineered to increase specificity and nuclease activity available. Engineered Cas9s have ...Xie, H., Liu, D., Mo, H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu, Z. (2022). FrCas9 is a CRISPR/Cas9...Basic Mechanisms Knockout Generation Multiplex Engineering Cas9 Cas9 Orthologs Anti-CRISPR Small Edits HDR...scientists have modified for a wide range of genome engineering techniques . Collectively, these techniques are...R epeat) technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) ...mutation within the targeted gene. However, the strength of the knockout phenotype for a given mutant cell...
Showing: 41 - 60 of 795 results