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  1. Lentivirus Plasmids

    Type
    Collection
    ... ORF expression, multicistronic design enables tracking of viral transduction. Also see plasmid 46948 ...inducible knockdown, multicistronic design enables tracking of viral transduction. See article for more similar...

  3. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes remaining...-50 μL of TE. Top Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated...it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and before step...

  4. CRISPR References and Information

    Type
    Collection
    ...and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF 47.5 KB Vosshall...RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) ; PDF 248.6...

  5. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...generation of shRNA transcripts. cPPT Central polypurine tract, cPPT, improves transduction efficiency by facilitating...competent cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma...at 37°C for 2 hours. Purify with Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Digest eluate... the DNA. Purify the DNA using a Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Measure the DNA...AB01200-02000, LB contains: 10g tryptone 5g yeast extract 10g sodium chloride 15g agar Prepare LB agar solution...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...superfamily, member 2) DIF, TNF-alpha, TNFA, TNFSF2 TRAC T cell receptor alpha constant - TRAJ1 T cell receptor...receptor, beta - IL16 interleukin 16 (lymphocyte chemoattractant factor) FLJ16806, FLJ42735, FLJ44234, HsT19289...receptor superfamily, member 10c, decoy without an intracellular domain CD263, DCR1, LIT, MGC149501, MGC149502...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...be lysed or the substrate must be delivered intracellularly in order to measure luminescence. Gaussia luciferase...Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created by fusing NanoLuc® and...

  8. Brain Initiative Collection

    Type
    Collection
    ...pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA...

  9. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...with an empty tube. Alternatively, you can just subtract the weight of the empty tube from the weight of...the (Link opens in a new window) QIAquick Gel Extraction Kit . Always follow the manufacturer's instructions...

  10. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If your lab has pre-...mL Kanamycin 50 µg/mL Spectinomycin 50 µg/mL Tetracycline 10 µg/mL Tips and FAQ What is the difference...

  11. Centrifugation

    Type
    Protocol
    ...experimental procedures, such as minipreps and RNA extractions. Plus, centrifuges are useful for simply collecting...cousins, but can hold much larger containers. Ultracentrifuges are also generally floor sized centrifuges...

  12. Pouring LB Agar Plates

    Type
    Protocol
    ... g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium chloride.../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated...

  13. Western Blot

    Type
    Protocol
    ... a sonication step may be required for hard to extract proteins such as those in the nucleus. Incubate...absorbance of the duplicate samples on the plate. Subtract the average absorbance of the blank from all of...

  14. Lentivirus ddPCR Titration

    Type
    Protocol
    ... min at 100 x g . Gently aspirate supernatant. Extract genomic DNA according to the GeneJet Genomic DNA...used to transduce HEK293T cells. Genomic DNA is extracted from the target cells and assayed for integrated...

  16. MAPK Plasmids

    Type
    Collection
    ...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...

  20. Fluorescent Protein Guide

    Type
    Collection
    ...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...
Showing: 41 - 60 of 508 results