...After production, all virus preps are titered and subjected to quality control by Addgene before being distributed...VP2 and VP3. Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed...Silver staining of purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins ...gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization purposes...

...pUCmini-iCAP-AAV9.452sub.LUNG1 AAV9.452sub.LUNG1 non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with... evolved AAV variant effective at targeting subventricular NSCs Schaffer 213968 AAV8R-12 Capsid AAV8R12...

...insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. The robust...are not secreted, so cells must be lysed or the substrate must be delivered intracellularly in order to ... luciferase engineered to use the red-shifted substrate Akalumine with significantly brighter signal and...

...Tirrell 62598 pKPY514 phenylalanyl-tRNA synthetase subunit E. coli p-azido-L-phenylalanine (Azf) C. elegans...127411 pEVOL-pylT-N346A/C348A PylRS M. mazei mono-substituted phenylalanine derivatives and tyrosinyl ethers...pMAH-POLY-eRF1(E55D) POLY-eRF1(E55D) E. coli para-substituted phenylalanine analogs Mammalian TAG Huiwang Ai...

...mammalian cells or zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One...mammalian cells or zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One...

...Service Addgene's Packaged on Request Service is subject to Addgene’s Terms of Purchase and the applicable... review your cargo/serotype selection after you submit your request, so we can set expectations to the...

...species (e.g., human, watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal...mutation, "active site", "active site loop", or "subunit interface") ID Plasmid Description Gene/Insert ...

...institution. Once the MTA has been signed, it can be submitted to Addgene through your user account or by email...materials from our repository. All purchases are subject to Addgene’s Terms of Purchase ....

...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary...190302 Anti-Gs protein, alpha subunit [N192/12] Gs protein, alpha subunit Cow Mouse IgG2a 190303 Anti-SAPAP1...validated for neuroscience research applications. A substantial number of these have been cloned, expressed and...References Andrews NP, et al. 2019. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

...requesting organization Viral vector preps remain subject to the terms and conditions of the applicable transfer...following plasmids are eligible for this service. Submit a request and we’ll let you know whether the item...

... the gene list below. For multiple isoforms or subunits, individual links to each gene page are provided...gene. For components with multiple isoforms or subunits, individual links to each gene page are provided...

...Protein Resources: Empty Backbones Biosensors Subcellular localization Optogenetics Background Förster ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses...

...and death of dopamine-producing neurons in the substantia nigra, one of the movement control centers in...of fluorescent proteins, including Markers of Subcellular Localization, Optogenetics Plasmids, and Chemogenetics...

...to uridine, which is read as thymidine during subsequent DNA repair, creating a C to T change. Modern ...sequence will remain unchanged and available as a substrate for another round of prime editing. Figure 6: ...Recombinases and Integrases Recombinases, and their subfamily integrases, are used for large scale edits. These...Whatever the chosen epitope tag, the locus is subsequently isolated by affinity purification. After purification...

...whether the edit was successful. PCR amplification, subcloning and Sanger sequencing (for HDR or NHEJ): Provides...PCR amplification of targeted region from DNA, subcloning into a plasmid, and screening individual clones...

...et al. ’s classification defines 5 types and 16 subtypes based on shared characteristics and evolutionary... with Type V following in 2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain ...

...primary article to confirm details and outcomes. Submit Validated gRNA Sequences Validated gRNA sequences...email the file to [email protected] with the subject heading "gRNA sequence spreadsheet". Thanks for...

...active promoter like CAG, or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre...sites AAV Uchida Cre Reporters and Tools In this subtype of loxP plasmids, reporter genes indicate which...

...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics Background...

...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab of Michael ...