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Showing: 1 - 20 of 58 results

  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...proteins tagged with genes or peptides with known subcellular localization patterns. Useful for co-localization...Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization More Fluorescent Protein Resources...Cytoskeletal Junctions Secretory Pathway Background The subcellular localization of a protein is often tied to its...There are a variety of methods for determining subcellular localization. One of the most common is to use...proteins that are targeted to various organelles or subcellular structures. You should fuse your protein of interest...The cell contains a variety of organelles and subcellular structures each with their own unique function...

  2. Resolute Plasmid Collection

    Type
    Collection
    ...pDONR221). These vectors allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ... expressing these tools in well-defined cellular sub-populations has generally been limited to single-...recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic...opens in a new window) Hafner G, Witte M, Guy J, Subhashini N, Fenno LE, Ramakrishnan C, Kim YS, Deisseroth...projections of molecularly defined dopamine neuron subtypes using intersectional genetic approaches. Nat Neurosci...

  5. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...enzymatic binding to attach substrate Control plasmids with a recognizable sub-cellular localization (membrane...information on substrates and synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature...simultaneously with dual labeling when used with two substrates Can be used in live or fixed cells, and for internal...protein tags based on the acyl carrier protein. Substrates are derivatives of Coenzyme A (CoA). These tags...252 KB) ACP and MCP tags ACP-MCP-Tag plasmids - Substrate source (PDF, 46 KB) Please note: New England Biolabs...product portfolio, including the CoA-derivative substrates and the ACP and SFP Synthases. Users may still... their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme A disodium salt from a...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ... NTCP2 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) ABC17, ABCB2, APT1, D6S114E, FLJ26666... TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) ABC18, ABCB3, APT2, D6S217E, PSF2...receptor subfamily C, member 3 NKG2-E, NKG2E KLRC4 killer cell lectin-like receptor subfamily C, member...macropain) 26S subunit, ATPase, 3 MGC8487, TBP1 PSMC4 proteasome (prosome, macropain) 26S subunit, ATPase, ...) 26S subunit, non-ATPase, 10 dJ889N15.2, p28 PSMD11 proteasome (prosome, macropain) 26S subunit, non-... 26S subunit, non-ATPase, 14 PAD1, POH1, rpn11 PSMD2 proteasome (prosome, macropain) 26S subunit, non-...macropain) 26S subunit, non-ATPase, 3 P58, RPN3, S3 PSMD4 proteasome (prosome, macropain) 26S subunit, non-ATPase...

  7. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...resource subdivides the 110,139 human phosphoserine site library ( 188536 ) into 10 subpools of ~11,000...Pooled Library 188526 iSPI_pSer_Subpool#1 Pooled Library 188527 iSPI_pSer_Subpool#2 Pooled Library 188528...188528 iSPI_pSer_Subpool#3 Pooled Library 188529 iSPI_pSer_Subpool#4 Pooled Library 188530 iSPI_pSer_Subpool...Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library 188532 iSPI_pSer_Subpool#7 Pooled Library 188533...188533 iSPI_pSer_Subpool#8 Pooled Library 188534 iSPI_pSer_Subpool#9 Pooled Library 188535 iSPI_pSer_Subpool... can then be used to identify candidate human substrates of kinases of interest by mass spectrometry in... positional isomers are allocated to different subpools, allowing precise knowledge of phosphorylated ...

  8. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ... DSS, GTD, HHG, NROB1, SRXY2 nuclear receptor subfamily 0, group B, member 1 NR1D1 Plasmids NR1D1, EAR1...EAR1, hRev, THRA1, THRAL, ear-1 nuclear receptor subfamily 1, group D, member 1 NR1D2 Plasmids NR1D2, RVR...RVR, BD73, EAR-1R nuclear receptor subfamily 1, group D, member 2 NR1H3 Plasmids NR1H3, LXR-a, LXRA, RLD...RLD-1 nuclear receptor subfamily 1, group H, member 3 NR2E1 Plasmids NR2E1, RP3-429G5.1, TLL, TLX, XTLL...XTLL nuclear receptor subfamily 2, group E, member 1 NR2F1 plasmids NR2F1, COUP-TFI, EAR-3, EAR3, ERBAL3...NR2F2, SVP44, TCFCOUP1, TFCOUP1 nuclear receptor subfamily 2, group F, member 1 NR3C1 Plasmids NR3C1, GCCR...GCCR, GCR, GR, GRL nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) NR3C2 Plasmids...

  9. Ras Pathway

    Type
    Collection
    ...Catalytic subunit alpha B1,B2: non-catalytic subunit beta G1, G2, G3: non-catalytic subunit gamma PTEN...protein 1 PIK3 Catalytic Subunits PIK3CA PIK3CB PIK3CD PIK3CG Regulatory Subunits PIK3R1 PIK3R2 PIK3R3 PIK3R4...the gene list below. For proteins with multiple subunits or isoforms, individual links to each gene page...plasmids for the gene. For components with multiple subunits or isoforms, individual links to each gene page...receptor-related receptor IRS IRS1 IRS2 Insulin receptor substrate JUN Jun proto-oncogene KSR KSR1 KSR2 Kinase suppressor...bisphosphate 3-kinase catalytic and regulatory subunits - Class I PIN1 Peptidylprolyl cis/trans isomerase...Plexin B1 PPP1CA Protein phosphatase 1 catalytic subunit alpha PREX2 Phosphatidylinositol-3,4,5-trisphosphate-dependent...

  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service...blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Nat Commun. 2014 Jun ...genetically encoded sensors for population and subcellular imaging of cAMP in vivo. Nat Methods. 2022 Nov...FRET-based cGMP biosensor SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. Cell Rep...2021 Jun 29. pii: S2451-9456(21)00301-9. Takashi Tsuboi Glucose iGlucoSnFR-TS biosensor for intracellular...imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Maltose Single fluorescent protein biosensor for...imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Sucrose FRET-based sensor to monitor sucrose levels...

  11. mTOR Pathway

    Type
    Collection
    ...isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB PIK3CD PIK3CG Regulatory Subunits PIK3R1 PIK3R2 PIK3R3 PIK3R4...and mTORC2. Each complex contains a unique subunit: raptor for mTORC1, and rictor for mTORC2. Background... the gene list below. For multiple isoforms or subunits, individual links to each gene page are provided...gene. For components with multiple isoforms or subunits, individual links to each gene page are provided...Neurofibromin 1 PRAS40 Also known as AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase and tensin homolog...bisphosphate 3-kinase catalytic and regulatory subunits - Class I PKC PKC alpha PKC beta PKC delta PKC...

  12. Mammalian RNAi Tools

    Type
    Collection
    ...(CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...(CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier ...1alpha promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...hPGK promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...hPGK promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier ...hUbiquitin promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...hPrion promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...

  13. Antibody Guide

    Type
    Collection
    ...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure...Isotype Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears...should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate after thawing and therefore...reaction in the presence of several different substrates. The luminescence produced by the reaction can...different species of origin, isotypes, and isotype subclasses of a primary antibody, which becomes the limiting...and block the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray film... cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...-v2 subpooled libraries 83980 — 83986 Activation Human Weissman 3rd 5 Varies hCRISPRi-v2 subpooled libraries...-v2 subpooled libraries 83998 — 84004 Activation Mouse Weissman 3rd 5 Varies mCRISPRi-v2 subpooled libraries...the entire genome or a specific class of genes (subpools). Species - the gRNAs within a given CRISPR library...KSHV) Cow ( B. taurus ) Library Size Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ...Activation Human Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043...

  15. Institute for Protein Innovation

    Type
    Collection
    ...Bind to the alpha subunit, outside the ligand-binding pocket Bind to the beta subunit, outside the ligand-binding...pocket Bind specific subunit pairs, outside the ligand-binding pocket Bind specific subunit pairs, blocking...mechanotransduction receptors, comprised of alpha-beta subunit heterodimers. The IPI collection contains antibodies...

  16. Bacterial Expression Systems

    Type
    Collection
    ...Manipulating the genome directly can give you more subtle control over protein expression and activity thereby... and lacO variants that can be expressed in B. subtilis Various combinations of N and C-terminal Strep... express a protein of interest in E. coli , B. subtilis and P. pastoris . All plasmids use the same restriction...or their derivatives. Binding between the RNAP subunit α fusion protein in pBRα-β flap and the λCI fusion...pB1H2w derivatives as a fusion to RNA polymerase subunit ω. Transcription factor binding to a putative promoter...

  17. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...enhanced transduction of the retina following subretinal administration and enhanced transduction and ...transduction and lateral spread in the retina following subretinal injection. It was identified by John Chiorini...transduction and lateral spread in the retina following subretinal injection as compared to the parental AAV44.9...

  18. Viral Production

    Type
    Collection
    ...After production, all virus preps are titered and subjected to quality control by Addgene before being distributed...VP2 and VP3. Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed...Silver staining of purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins ...gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization purposes...

  19. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...pUCmini-iCAP-AAV9.452sub.LUNG1 AAV9.452sub.LUNG1 non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with... evolved AAV variant effective at targeting subventricular NSCs Schaffer 213968 AAV8R-12 Capsid AAV8R12...

  20. Luciferase Plasmids

    Type
    Collection
    ...insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. The robust...are not secreted, so cells must be lysed or the substrate must be delivered intracellularly in order to ... luciferase engineered to use the red-shifted substrate Akalumine with significantly brighter signal and...
Showing: 1 - 20 of 58 results