... out Joanne's Reddit AMA Here are 6 steps to submitting a resume that will give you a chance at an interview...interview:  1) Have an excellent resume ready to be submitted The structure and content of a resume can be crucial...the hiring group.  Read here for more on timely submission of a job application. 3) Read job descriptions... not likely know the exact person monitoring submissions (usually a Human Resources representative). Pro...

...and selected subcellular marker proteins, it’s possible to get an idea about the subcellular structures ...protein function. Getting ready to determine the subcellular localization of your protein of interest The ... vesicles via the secretory pathway to their subcellular or extracellular destination. At the end of their...proteins tagged with FPs to highlight various subcellular structures. Please see Figure 1 for commonly ...inert fluorescent protein fusions to visualize subcellular structures. For example, you might use your fluoresent...particular gene. For subcellular markers in yeast see our plasmid collection from...You can find more plasmids for labeling your subcellular structure of interest in the Allen Institute ...

...Plasmid 1: B. subtilis knock out and knock down libraries. Listen to Our segment on the B. subtilis libraries...you'll learn about new libraries for studying B. subtilis biology, plasmids for enhancing iPSC production... b. subtilis...

...where your plasmid yield is sub-optimal? If you are consistently getting sub-optimal plasmid yields from...your stab from Addgene, streak for single colonies, sub-culture, and prep with a DNA prep kit or your lab's...plasmid yield. Start with a single, fresh colony Sub-culturing directly from a frozen glycerol stock or...

...Visualization of neuronal sub-cellular structures GFP is limited in its ability to label subcellular structures in...be used to amplify the direct smFP signal within sub-cellular structures. For instance, smFPs have been... be very effective probes for finely visualising sub-cellular structures or low-abundance proteins. When...

...of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences under...temperature variations and heat transfer at the subcellular level requires a probe with fast kinetics and...

...Chylinski (who coincidentally speak the same Polish sub-dialect making their Skype communications extra fun...

...repair template strategies activate different HDR sub-pathways like synthesis-dependent strand annealing...DNA repair (with bonus gRNA design resources.) Subscribe to CRISPR updates on our blog to keep current ...

...provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and... calculate the amount of bound ligand (after subtracting out non-specific binding). Historically, glutathione-S-transferase...to express a foreign protein in bacteria and subsequently purify the fusion protein from a bacterial extract...extracellular domain of the erythropoietin receptor and subsequently evaluated interactions with mutant and wild-...

...regular group meetings, journal clubs, and focused sub-group meetings to discuss day-to-day progress, ideas...

...direct your biosensor to specific tissues or cell (sub)types for expression and downstream monitoring. Nervous...

...For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than in the cytosol...population of cells (e.g., a neuron subtype) and specific subcellular compartments for measurements. All...Localization The calcium concentrations in different subcellular compartments can vary greatly. Additionally, ...changes you will observe in your experiment could be subtle or dramatic. You want to use a calcium sensor that...allosteric rearrangements of the sensor, and (3) subsequent change in fluorescence only occurs at high calcium...sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream monitoring...

...the desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer...little flexibility in the location of the cut and subsequent edit. In these applications, location takes priority...requirement to allow flexibility in gRNA selection and subsequent editing. Identifying PAM-free and -flexible Cas...

... genome-wide CRISPR libraries and a selection of sub-libraries targeting specific classes of human genes...

...of stochastic magic and Poisson statistics, each sub-population has, on average, one distinct enhancer...

...source - find a lab within your own neuroscience sub-discipline that has published using the technique... behavior (activation of the circuit promotes subsequent, repeated activation), this is an approximation...behavioral effect but the function of specific subregions within the hippocampus were only recently studied...

...assist with proper protein folding, and deal with sub-optimal codon usage (Table 1) A few mutations are...

...a Good Conflict Resolver? The first and foremost sub-skill to have here is pretty intuitive: You need ...also be categorized as either “personal” or “substantive”. A personality-based conflict occurs when two...a lab or work environment if left unchecked. Substantive conflict occurs when people or teams who normally...”) when in fact many sources of conflict are substantive (“Person C told Person A one thing and inadvertently...compromise and arbitration might remedy short-term substantive conflicts, but they could also aggravate long-term...

... biosensors can be imaged at spatial scales from sub-cellular to whole-body in small animals, such as ...intensities in NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps...antigen-dependent fluorescent nanobodies. Nat Methods submitted (2021). Tchekanda, E., Sivanesan, D. & Michnick...

...adding different ligands to the same sample to label sub-populations of your protein of interest. For example...range of useful molecules linked to that substrate. If the substrate was linked to, say, a chemical dye or...instead become trapped in a covalent bond with its substrate. Then, all it takes is a bit of chemistry magic... gets covalently linked to derivatives of its substrate O6-benzylguanine (BG) (Keppler et al., 2003). ...be labeled with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005)...., or other uses of your protein, such as with substrate-linked PEG, biotin, or magnetic beads. In one ...