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Showing: 41 - 57 of 57 results
  1. Hot Plasmids - February 2022

    Type
    Blog Post
    ...anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag fusions, while the anti-...
  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression iRFP (aka iRFP713) 690 713 6 4.5 2.8 hr Dimer piRFP - Mammalian Expression pBAD/His-B-iRFP - Bacterial...Mammalian Expression mCyRFP1 528 594 18 5.6 Monomer pNCS-mCyRFP1 - Bacterial Expression mCyRFP1-C1 - Mammalian...Expression mRFP1 584 607 13 4.5 1 hr Monomer pcDNA3-mRFP - Mammalian Expression pMXs-mRFP1 - Mammalian...Expression pTagRFP657-C1 - Mammalian Expression pFA6a-link-yoTagRFP657-Kan - Yeast Expression smURFP + BV 642...Plasmids miRFP670 642 670 12 4.5 Monomer pmiRFP670-N1 - Mammalian Expression pBAD/His-miRFP670 - Bacterial...Expression iRFP670 643 670 13 4 ~5 hr Dimer piRFP670-N1 - Mammalian Expression pBAD/HisB-iRFP670 - Bacterial...Expression iRFP682 663 682 10 4.5 ~5hr Dimer piRFP682-N1 - Mammalian Expression pBAD/HisB-iRFP682 - Bacterial...
  3. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... piRFP682-N1 iRFP702 673/702 7.6 piRFP702-N1 miRFP703 673/703 8 pmiRFP703-N1 miRFP709 683/709 4 pmiRFP709...Find Plasmids iRFP670 643/670 12.7 piRFP670-N1 miRFP670 642/670 12 pmiRFP670-N1 iRFP682 663/682 10.2 piRFP682...pmiRFP709-N1 iRFP (aka iRFP713) 690/713 6.2 piRFP iRFP720 702/720 5.8 piRFP720-N1 iSplit 690/713 5.3 pPAS-...Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation) 3.2 pPAiRFP1-N1 PAiRFP2 692/719 (after photoactivation...BphPs). Spectrally distinct permanently fluorescent iRFP variants have been shown to have high effective ...single or multicolor imaging. Photo-activatable iRFPs can be ‘turned on’ by non-phototoxic far-red light...living animals. Further development of the original iRFP has resulted in a split fluorescence complementation...
  4. Hot Plasmids - August 2020

    Type
    Blog Post
    ...inducible promoter. The gRNA piggyBac plasmids include a mRFP reporter. With this system, they could quantify ...
  5. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...AAVS1-mTagRFPT-CAAX AICSDP-42 mTagRFPT CAAX domain of K-Ras Plasma Membrane 114403 LMNB1-mTagRFP-T AICSDP...AICSDP-35 mEGFP NA Cytoplasm 101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrioles 101782 LAMP1-mEGFP...protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T AICSDP-28 mTagRFP-T Alpha-tubulin Microtubules 101786 ST6GAL1...Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T Nucleophosmin Nucleolus (granular component...AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP NA Cytoplasm 114405...
  6. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ....CB7.CI.TurboRFP.WPRE.RBG James M. Wilson AV-9-PV2177 105548-AAV9 pENN.AAV.CMVs.TurboRFP.WPRE.RBG James... M. Wilson AV-1-PV2177 105548-AAV1 pENN.AAV.CMVs.TurboRFP.WPRE.RBG James M. Wilson AV-1-PV2642 105552-...105552-AAV1 pENN.AAV.hSyn.TurboRFP.WPRE.RBG James M. Wilson AV-1-PV2975 105622-AAV1 pAAV.CamKII(1.3).eYFP.WPRE.hGH... M. Wilson AV-5-PV2177 105548-AAV5 pENN.AAV.CMVs.TurboRFP.WPRE.RBG James M. Wilson AV-5-PV2213 105547-... M. Wilson AV-8-PV2177 105548-AAV8 pENN.AAV.CMVs.TurboRFP.WPRE.RBG James M. Wilson AV-9-27056 27056-AAV9... M. Wilson AV-5-PV2642 105552-AAV5 pENN.AAV.hSyn.TurboRFP.WPRE.RBG James M. Wilson AV-5-PV2820 100838-... M. Wilson AV-9-PV2642 105552-AAV9 pENN.AAV.hSyn.TurboRFP.WPRE.RBG James M. Wilson AV-9-PV3080 100840-...
  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes tagRFP Ebert pFGC-pcoCas9...Wolfe pLKO5.sgRNA.EFS.tRFP657 57824 Mammalian/Lentiviral BsmBI none S. pyogenes tagRFP657 Ebert pL-CRISPR.SFFV.GFP...EGFP Ebert pLKO5.sgRNA.EFS.tRFP 57823 Mammalian/Lentiviral BsmBI none S. pyogenes tagRFP Ebert pL-CRISPR.SFFV.tRFP...Mammalian/Lentiviral BsmBI yes, cut S. pyogenes tagRFP Ebert pSAG1::CAS9-U6::sgUPRT 54467 Other/Toxoplasma...
  8. Control AAV Preps

    Type
    Collection
    ...9, rg*, PHP.eB Wilson 105548 pENN.AAV.CMVs.TurboRFP.WPRE.RBG CMV TurboRFP Constitutive 1, 5, 8 Wilson ...Constitutive 5 Wilson 105552 pENN.AAV.hSyn.TurboRFP.WPRE.RBG hSyn TurboRFP Constitutive 1 Wilson 105556...
  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...epegRNA BsaI No mRFP1 David Liu pU6-tmpknot-GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu U6-...pegRNA-GG-acceptor Mammalian hU6 pegRNA BsaI No mRFP1 David Liu QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA...
  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Christien Merrifield 27700 Hip1R-tDimerRFP-N1 Hip1R RFP CMV Parkinson's Christien Merrifield 28195...Lazarou 119693 pBMN iRFP670-OPTN(D474N) OPTN iRFP670 ALS Michael Lazarou 119694 pBMN iRFP670-OPTN(F178A/D474N...Clifford Brangwynne 122441 pHR-FUSN-miRFP670-Cry2WT FUS Cry2WT, miRFP670 SFFV ALS Clifford Brangwynne 122668...D474N) OPTN iRFP670 ALS Michael Lazarou 120163 mCh-KIF5A*-strep KIF5A mCherry, Streptavidin CMV ALS Juan...
  11. Luciferase Plasmids

    Type
    Collection
    ...104587 pHIV-iRFP720-E2A-Luc Firefly EF1α Lentiviral expression of firefly luciferase and iRFP720 from a bicistronic...
  12. Adenovirus Plasmids

    Type
    Collection
    ...Zhang 50957 RedTrackCMV Shuttle For production of mRFP-trackable viruses containing transgene under CMV...
Showing: 41 - 57 of 57 results