...puncta (GFF positive/RFP positive), with these puncta turning red (GFP negative/RFP positive) upon fusion... estimated by the GFP/RFP ratio: a decrease in GFP fluorescence relative to RFP fluorescence suggests ...relative to RFP fluorescence suggests a decrease in autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid...decrease in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that...autophagy biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein...fluorescent proteins: a relatively pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive...molecule of GFP-tagged LC3 for every one molecule of RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is...

... the default RFP and CFP, while Brainbow 2.1 switches from the default GFP (green) to RFP, CFP, or YFP...technology Brainbow. Brainbow 1.0 used the fluorophores RFP (red), YFP (yellow), and membrane-tethered CFP (cyan...of genes farther downstream in the construct. As RFP and OFP are directly downstream of the promoter in...activate fluorescence of the downstream OFP, GFP, or RFP. Cells that express the construct but do not undergo...construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of a ubiquitous promoter...GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific cell type. The Confetti mouse was first...construct, a variant of Brainbow 1.0 that contains RFP, CFP, and YFP. After crossing onto a tamoxifen-inducible...

...secondary antibody staining HA Tagged GFP, RFP, H2A2B Myc Tagged RFP, H2A2B Chicken IgY (Y) Labeled Anti-Chicken...sequences that targeted the following antigens: GFP, RFP,  the histone protein H2A2B, and the actin-binding...Amplifying weak signals of fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution across synaptic partners... only stain with one HRP-RANbody at a time GFP, RFP, H2A2B, Gelsolin Spaghetti Monster: HA (H) or Myc...staining Requires secondary antibody staining GFP, RFP, H2A2B   Are you ready to start making your own...

...Segment on these new AAV Vectors Hot Plasmid 3: mCyRFP1 - a new, monomeric, and cyan-excitable red fluorescent...fluorescent protein. Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric...

... an RFP protein that binds to the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the...64542 or 64543) Before translation, both GFP and RFP are bound to the reporter, causing it to appear yellow...translational repression. During later stages, single RFP-labeled osk and Oskar protein were detected at the...

...sequence is also found in the reporter gene). A control RFP expression plasmid.  With this set up, all successfully...successfully transfected cells will express RFP while cells containing the reporter gene that was cleaved...be cut and repaired by NHEJ as well. The ratio of RFP to GFP expressing cells provides a real-time read...developed a modified monomeric red fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently...read out of Cas9 activity.  By using the RFP:GFP ratio, edited cells can be sorted from unedited cells. ...

...many of the RFPs developed from natural ancestors), and screened through libraries of new RFP variants for...fluorescent proteins (RFPs) and, while the authors note that there are other RFP variants available that...deliberate and random mutagenesis to isolate the best RFP. They targeted known residues on the outer surface...fluorescence, brightness, and proper maturation. Three new RFPs were isolated and characterized: mScarlet (fluorescent...RDSmCherry1: Engineering and directed evolution of new RFP variants The toolbox of fluorescent proteins (FP)...of mCherry and the spectral properties of various RFPs. For tips on how to choose the right FP for your...set of CyRFP constructs available at Addgene includes bacterial and mammalian mCyRFP1 or CyRFP1 expression...

...proteins: RFP (red), YFP (yellow) and M-CFP (membrane tethered cyan). Without Cre recombination, RFP is expressed...construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem...default” state; for example, Brainbow-1.0 expresses RFP when the construct has not undergone recombination...

...cell, the lab fused the red fluorescent protein (RFP) to a self-assembling protein (SAP) such as a bacteriophage...induced with arabinose to begin expressing the SAP-RFP fusion. Then, the cells are washed to stop the production...

...fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are also...selection of transformants based on expression of RFP in the seed (Emami, Yee and Dinneny, 2013). Preparing...

... the leader sequence. Using a variety of GFP and RFP fusions as their test genes, the researchers at the...terminators of varying strengths centered within GFP/RFP bicistronic reporters characterized in Cambray et...

...cells, each line showed broad recombinase-dependent RFP expression. Even at low expression levels, recombination...Reporter constructs equivalent to site-STOP-site-myrRFP, with “site” representing the specific recombination...

...hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI restriction...

... imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI /...#49004) to the spectra of both mCherry and TagRFP.  Here, the TagRFP spectrum is shown in the darker colors...peak of the TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission ... emission. For this filter set, we would expect TagRFP to give a brighter signal than mCherry. In general...shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like ... or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent protein such as ...

...rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / A58T / K69N / L84F /... working on in vivo imaging models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs...I203R) (2) and PA-mRFP1(S146H / I161V / I197H) (3) were derived from DsRed and mRFP, respectively. When...

...recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest. Pairs of identical...

...amounts of non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could...

...fluorescent proteins: a relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant,...

...which recruit fluorescent proteins BFP, GFP, and RFP fused to the respective hairpin binding domain. Pairs...

...changes in a protein.    Cells expressing an RFP reporter plasmid for recombinase activity were exposed...