...high-titer 100 µL solutions of purified viral particles that are suitable for in vivo use. Learn more ...AAV are distributed as 100 µL of purified viral particles in solution that are suitable for in vivo use....

...infectious titer, meaning the concentration of viral particles that can transduce cells, which can vary based...physical titers, meaning the concentration of viral particles containing a viral genome. While ddPCR is superior... sequences. Human Gene Therapy Methods, 23(1), Article 1. https://doi.org/10.1089/hgtb.2011.034 Benyamini...in the CNS. Molecular Neurodegeneration, 15(1), Article 1. https://doi.org/10.1186/s13024-020-00361-z Grimm...& Kleinschmidt, J. (1999). Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes...production of recombinant AAV-2. Gene Therapy, 6(7), Article 7. https://doi.org/10.1038/sj.gt.3300946 Howard... 1 Vectors. Human Gene Therapy Methods, 28(1), Article 1. https://doi.org/10.1089/hgtb.2016.180 Korte,...

...of the vector particle, some of the RT protein gets packaged inside of the vector particle, along with ...you consider that out of the 6 x 106 virus-like particles that we come into contact with every day (Prussin...understanding of the structure of a wild-type viral particle — and of the normal, replicative life cycle — ...detailed view of the structure of a retroviral particle, which is essentially the same for viruses and...How it is, exactly, that heaps of viral vector particles are made while missing the polymerase gene may...envelope during exocytotic budding, as the viral particle emerges. Retrovirus life cycle Retroviruses go...cycle” in order to understand what these vector particles do once they come in contact with target cells...

...to your article. Note that if you entered a deposit description, this will become your article title. ...Figure 4: Add plasmids to the article by clicking “Add Plasmid to this Article” (left) and entering a plasmid...publication where you described them. Search for your article as you would search for it in PubMed and choose...pages and scientists will be asked to cite this article when using your plasmids. You can submit "Unpublished...sequences, and/or maps. Add plasmids to your article After you’ve selected your publication or entered...description/experimental purpose (right). On the article page, enter the name, type, and description of ...next-generation sequencing. Please see the help center article, "Can I deposit a pooled DNA library with Addgene...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...plasmids you need. Below you'll find our hot plasmid articles from April 2018. If you'd ever like to write about...-Away: Targeted endogenous protein degradation Article contributed by Mary Gearing Listen to the TRIM-Away...29153837. Endosomal pH sensors for STED nanoscopy Article contributed by Eric J. Perkins Listen to Endosomal...microscopy, comes from his recent Nature Communications article “SRpHi ratiometric pH biosensors for super-resolution...biosensors used to detect the maturation of neutral vesicles to more acidic lysosomes. Though a number of biosensors...microscopy techniques, the first author of this article, Dr. Douglas Richardson, recently contributed a...

...vectors consist of full particles (white arrowhead) relative to empty particles (black arrowhead). Scale...produced your viral vector lot. At Addgene, AAV particles are titered by droplet digital PCR (ddPCR) using...of empty to full (i.e., genome-containing) AAV particles within representative vector preparations was ...microscopy after negative staining. Empty vector particles can be identified after negative staining and ...and appear darker than full vector particles.     Figure 3: Electron micrograph of AAV vector preparation...prior to being frozen. Specifically, lentiviral particles are collected from the lentiviral preparation ...centrifugation. Precipitated pellets containing viral particles are then resuspended in PBS, aliquoted, frozen...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... Light-mediated block of synaptic transmission Article contributed by Klaus Wanisch  Listen to the optogenetics...noninvasive imaging of microbes in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic...ultrasound. These ARG plasmids contain genes for “gas vesicles,” a gas-filled protein structure that water-bound... Shapiro lab discovered in 2014 that these gas vesicle proteins scatter sound waves and create ultrasound...Typhimurium. This work demonstrates engineered gas vesicles as the first reporter genes using ultrasound and...on mechanisms of miRNA-mediated gene regulation Article contributed by Michael Lemieux  Listen to the miRNA...

...authors, year, narrow by article type (review, patent, clinical study, research article etc). There are also...can generally take you to the full text of the article – via the journal (though you may need to be on...literature search, try to find a recent review article to give you an overview of the topic and point ...point you to relevant primary research articles. Then just start reading and following citations through the...introductions should be fairly focused. Consult a review article for a broader scope of what’s going on in any given...probably won’t get much attention reading through an article for the first time. The first time through, just...builds up to an overall conclusion(s). In some articles, the data are presented to build a case for a ...

...the blog articles come up as well as their topic category. New author pages to find articles from your... to the new Addgene blog! Same URL, same great articles to help you with your experiments, but a new look... molecular biologists alike. Now with over 600 articles, we needed a better way to organize and present...try the search bar Now, you can search for the articles you want to find without even leaving the Addgene...behind the blog post and find more of their work. Articles from guest bloggers will have the author information...information at the top and bottom of their article. We hope you’ll join the conversation! Let us know if ...you up-to-date. Be the first to know about new articles on the Addgene blog by subscribing to instant ...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... tagging of proteins for degradation using AID Article contributed by Alyssa Cecchetelli Auxin Inducible...targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely... channelrhodopsins for the optogenetic toolbox Article contributed by Susanna Bachle Anion-conducting ...identified MerMAIDS from their analysis of different publicly available metagenomic data including the Tara ...demonstrates the power of combining the analysis of publicly available metagenomic datasets with biophysical...PMID: 31346176. Using LADL to loop with light Article contributed by Eric Perkins Mammalian genomic DNA...

...Cas9 RNP delivery vehicle that’s based on mouse leukemia virus (MLV) virus-like particles (VLPs). VLPs lack...are engineered murine leukemia virus (MLV)-like particles loaded with Cas9-gRNA ribonucleoproteins (RNPs...their tool Nanoblades because they think of these particles as tiny DNA cutting ninjas that deliver CRISPR..., multimerize and spontaneously assemble into particles at the cell membrane. Mature Nanoblades then bud...the Gag-Cas9 fusion protein. A single Nanoblade particle can be loaded with 1-4 different gRNAs. Viral ... DNA can be generated by incubating Nanoblade particles with polybrene, a cationic polymer. Polybrene ...surface, and also helps form DNA and retroviral-particle or VLPs complexes. The team found that "all-in-one...

...notice. A 2009 research study found that 12% of articles in medicine, 27% in natural sciences, 82% in humanities... But these continue to rely on printed journal articles as the dominant tool for sharing ideas. This leads... us, such as finding time to read all of these articles. Most of us hardly have time to keep up with all... them on their own. According to a Nature News Article, since the 1980s researchers have been increasingly... outside of their disciplines. Included in the article is an interactive chart where you can see the yearly...useful tool for turning video views into online article downloads – especially if they are published in...to learn more, you can then link to the journal article, find blogs about the study, or even review Facebook...

...preps in our repository through our Hot Plasmids articles. Here's what you'll find in this post: MagIC-Cryo-EM...making it easy to identify dozens or hundreds of particles surrounding each of the highly-visible beads on...collected with MagIC-cryo-EM. Nucleosome-like particles identified by Topaz software (green circles), ... from AWI by helical LEAs. C-E) cryo-EM single-particle analysis of PRC2 with RvLEAMshort (1:6 molar ratio...glutaraldehyde crosslinking: representative micrograph (C), particle orientation distribution and Fourier-shell correlation... addition of LEAs may bias the distribution of particle orientations, this could be overcome with methods...prime editors (PEs) via engineered virus-like particles (eVLPs) offers an option to transduce cells directly...

...Physical titer (particle count, rAdV genomes/mL) can be measured by: Optical density: particle concentration...260nm corresponds to 1.1 x 1012 particles/mL. To calculate the particle number: OD260 reading x dilution...dilution factor x 1.1 x 1012 particles = number of particles per mL of sample. The purity of the sample can...vectors The adenovirus virion is a non-enveloped particle of ~100 nm, consisting of a capsid surrounding...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...Expanding the optogenetics toolbox with CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust...novel opsin that enables single-cell optogenetics Article contributed by Meghan Rego  Listen to the soCoChR...29184208.        Synthetic decaffeination operon Article contributed by Angela Kaczmarczyk  Listen to the... for live-cell imaging of biochemical activity Article contributed by Angela Holmes Jin Zhang’s lab is...Listen to the FLIM-FRET acceptor podcast segment Article contributed by Christina Mork The Murakoshi lab...

.... Physical titers measure the amount of viral particles in a sample and are usually based on the presence...infectious titers, measure how many of the viral particles produced can actually infect cells. These assays...whether or not it is incorporated into a viral particle. Consequently, titers based off of p24 quantification...they can include free p24 and defective viral particles. Direct measurement of lentiviral RNA is one alternative...prep as the measurement will include defective particles. Studies have found that values can be 10-1000...quantify virus as it only measures infectious viral particles. One of the si mplest methods of measuring functional...distinguish between a cell that has had one viral particle integrated into its genome and a cell that has...

...a lot of time in PubMed searching for research articles? Well then, my scientist friend, do I have a handy...few quick steps, you can use PubMed to see which articles contain plasmids deposited at Addgene from the... Plasmids” badge, you’ll know that within that article you’ll find new plasmids. Simply click on the badge...site (Side note: I always recommend you read the article to ensure you understand the features of the plasmids...log-in to your PubMed account and are browsing article abstracts you should see the “Find Plasmids” box...the Literature How to Write a Scientific Review Article Tips for Improving Your Next Manuscript Resources...

...to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-... on the average number of citations for recent articles. Of course, a publication in Science or Nature... citation counts for a moment, it’s clear that articles in less-prestigious journals have been gaining...Journals” found that the proportion of highly cited articles appearing in lower-prestige journals has risen...impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649    ...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...here.  Human whole genome sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial...Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein Sparse Predictive...GFP reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a ...TALE-fluorescent protein fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double...

...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...microtubule-associated protein to regulate cell growth dynamics Article contributed by Klaus Wanisch  Listen to the optogenetics...for visualizing RNA production and localization Article contributed by Tyler Ford  Listen to the riboglow...tools for developing new FPs - the mCarmine story Article contributed by Caroline LaManna  Listen to the ...quantification of base-editing and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk  Listen to the...