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  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven...TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600; CMV-driven SaCas9 60957 : PX551; Truncated MeCP2 promoter-driven...driven by either the ubiquitous cytomegalovirus (CMV, PX601 [#61591] ) or liver-specific tyroxine binding...

  2. CRISPR References and Information

    Type
    Collection
    ...packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF 2.3 MB Zhang GeCKO pooled library amplification...packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Kits are also available (mouse or human libraries...

  3. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W...

  4. Lentiviral Guide

    Type
    Guide
    ...fused to a heterologous enhancer/promoter such as CMV or RSV Return to Top Viral Production The process...and thus requires an RNA pol II promoter, such as CMV or RSV. What techniques can be used to clone an insert...hybrid 5' LTR with a constitutive promoter such as CMV or RSV. 3' LTR in cis Terminates transcription started...

  5. Qi Lab CRISPR Page

    Type
    Collection
    ... the genome. The pgRNA_humanized also contains a CMV-puro-t2A-mCherry expression cassette, useful for ...

  6. Mammalian RNAi Tools

    Type
    Collection
    ...pLL3.7 Expresses shRNA under the mouse U6 promoter; A CMV-EGFP reporter cassette is included in the vector ...

  8. Promoters

    Type
    Guide
    ...Used in Research Promoter Expression Description CMV Constitutive Strong mammalian promoter from human...

  9. Plan Your Experiment

    Type
    Collection
    ... vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter...

  10. Plan Your Experiment

    Type
    Guide
    ... vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter...

  11. Caltech Systemic Capsids

    Type
    Collection
    ...CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV....

  12. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab...Note: pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For...

  13. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    .... Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab.... It can be used with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral..., this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral...

  14. Export Control Regulations Lifted for Rabies and VSV-G plasmids

    Type
    Blog Post
    ...VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope protein for producing...Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral...pseudotyping NanoMEDIC particles Akitsu Hotta pHCMV-RabiesG Contains Rabies virus G protein; see depositing...

  16. Pooled Library Amplifications

    Type
    Blog Post
    ... Protoc. 2017 with modifications Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled...1866 bp, plus a 20 bp barcode 400 ng Solid pCMV-Rep 78/68 SSM Library Amplification and Sequencing...

  17. New Viral Vectors - March 2024

    Type
    Blog Post
    ...Chemogenetics Deisseroth New viral vector pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral ...
Showing: 41 - 60 of 70 results