... genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The Joung lab has also modified their...use in mammalian cells SpCas9 variants with no detectable off-target effects Modified PAM specifity of ...

...Viral Vector Resources Addgene's Data Hub Curated technical reports from the research community demonstrating...troubleshooting advice and explanations of viral vector technologies. Penn Vector Core AAV Vectors Many AAV vectors...

...inclusive, ‘open-access ethos’ to the results, techniques and reagents with the highest-possible quality... research as well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project...

...pmax-DEST [48222] and (Link opens in a new window) Lifetechnologies Gateway Destination Vectors ) to adapt to ...pmax-DEST : Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector....

...plasmids in the future. AVEXIS: One popular method to detect interactions is AVEXIS (for Avidity-based extracellular...Interaction Screening (AVEXIS) for the scalable detection of low affinity extracellular receptor-ligand ...

...intellectual property rights, and provides liability protection for material providers. Addgene’s deposit agreements...distribution. Please see the Sample Agreements on our Technology Transfer Information page for more information...

...exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA - additional protection added ...

...CRISPR Guide for a general introduction to CRISPR technology. Check out our CRISPR reference page for CRISPR...resources Bacterial CRISPR Resources The CRISPR technology was developed from bacterial immune systems, ...

...CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources include plasmids... website . Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's...

...can be safely handled at the lowest biosafety protection levels. We offer 961 in-stock viral vector preps...well-developed and trusted protocols and peer-reviewed techniques (Link opens in a new window) for producing high-quality...

... used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253... used with 2nd and 3rd generation lentivirus technologies. Looking for lentiviral plasmids to express ...

...neurons function. They are also delving into new technologies and resources in order to gain more fundamental...mitochondrial DNA using mitoBEs . Yi et al. Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV...

...traditional tagging methods. Several different tagging techniques, as well as the plasmids and protocols needed...Förstemann lab has developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate...

...zebrafish ( Sander et al., Nat Biotechnol. 2011 ; Huang et al., Nat Biotechnol. 2011). The table below lists...

...off-target effects. More information on each of these techniques can be found in our blog post CRISPR 101: Validating...genome editing in vitro and in vivo. Nature Biotechnology . 33 (1), 73–80. PMID: 25357182...

...Center at UCSF / QB3 Wiki Stepanenko et al. : BioTechniques, Vol. 51, No. 5, November 2011, pp. 313–327 ... No. 10, pp.1296-304 Kogure et al. : Nature Biotechnology, May 2006, Vol. 24 No. 5, pp.577-81 Shcherbo...

...tips for mammalian cell culture Aseptic Technique Aseptic technique is a critical skill for success in numerous... the key strategies of aseptic technique, including personal protective equipment, setting up a clean ...Ultracentrifugation Protocol Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once ...preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol Agarose Gel Electrophoresis Gel electrophoresis...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction... process when requesting samples from Addgene Technology Transfer How to Order A step-by-step walkthrough...

...avidity-based extracellular interaction screen to detect protein interactions, where the extracellular regions...

...wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat...

...Ligation) assembly method ( Sander et al., Nat Biotechnol. 2011 ). REAL utilizes a set of 28 plasmids each...

...activator system - Jaenisch Locus-specific ChIP technologies (iChIP & enChIP) using dCas9 - H. Fujii Disease...

...dividing cells. CRISPR Activate and Interfere technologies also indirectly modify local chromatin state...

...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...

...biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular signals, cells,...

...split fluorescence complementation probe used for detecting protein-protein interactions in vitro and in vivo...

... CRISPR Plasmids and Resources CRISPR technologies are continously developing and expanding. Addgene...

...Video Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Learn how to best protect yourself when...Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies to detect antigens...questions. Intro to the Lab Bench Introductory techniques designed to help you get started in the lab. ...Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer of your...

...constructs and routine protein and molecular biology techniques. Using a single protein with many available isoforms...

...epigenetic regulation ( Reyon & Tsai et al., Nat Biotechnol. 2012 ). The table below lists 84 pairs of these...

...synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource EraSynBio...