We narrowed to 876 results for: lic

1. Pushing the Limits of DNA Assembly

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   ...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

2. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   ...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and...

3. The Power Behind NGS Plasmid Validation: seqWell

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   ...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...of Toronto. He is an author of 20+ scientific publications and patents.   ...

4. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   ...identified MerMAIDS from their analysis of different publicly available metagenomic data including the Tara ...demonstrates the power of combining the analysis of publicly available metagenomic datasets with biophysical...

5. Revamp Your Lab Meetings With Creative Virtual Collaboration

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   ...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ...

6. Summer SciComm Series: A PhD in Science Communication

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   ...funded by the public and performed by researchers who received their educations from public schools and ...

7. Scientists Map the SARS-CoV-2-Human Interaction Network

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   ... we found in our map have an impact on virus replication. While we and our collaborators are starting ...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with...

8. The Importance of High Titer for AAV Transductions

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   ...you will have. When possible, use low-bind or siliconized tubes to store samples as it is more difficult...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

9. Fluorescent Proteins 101: Luciferases

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   ...identified in the sea pansy (Renilla reniformis), click beetle (Pyrophorus plagiophthalamus), deep-sea copepod...reniformis) Natural 481 36 Coelenterazine None No Click Beetle Red (CBR)(Pyrophorus plagiophthalamus) Natural...

10. Antibodies 101: Flow Cytometry Controls

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    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color...

11. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    ...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...

12. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    ... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ...

13. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...which is both small and potentially less likely to elicit a strong immune response, factors that may make...

14. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...light-mediated suppression of synaptic transmission in brain slices from mouse, and showed that it can alter motor ...

15. Viral Vectors 101: Biosensors

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    ...ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics, in this...Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct...

16. Antibodies 101: ChIP

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    ...next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions, and can give... upfront investment than many other antibody applications, this investment can well be worth it once you...

17. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...through many rounds of mutations, selection, and replications per day.  The PACE and PANCE systems use M13...

18. Top Requested Lentivirus and AAV of 2016

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    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ... PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers...

19. Antibodies 101: Fab Fragments

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    ... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...fragments described above can be used for similar applications and have similar advantages, though with some...

20. Choosing the B(right)est Fluorescent Protein: Photostability

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    ...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...