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TypeBlog Post... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his...
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Easing the Protein Purification Process with pCri
TypeBlog Post...introduction to pCri, please see the original publication cited below. pCri plasmids are available either...comprehensive review of affinity tags and microbial applications." Biotechnology journal 7.5 (2012): 620-634.... -
Science as Art
TypeBlog Post... new method to explore the inner structures of delicate plants and other organisms without destroying ...competition. This competition has gained so much public interest for its stunning images that is has been... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post...complete with all the fixings), as well as plenty of delicious desserts. We enjoyed a video highlighting the ...course of scientific research. What was once a complicated process of requesting and sharing plasmids has... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...Brazil. Through an expansion of our export license application service, we’re pleased to announce that we... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF.... Sensitivity In ex vivo studies on mouse brain slices, nLightG and GRABNE were more sensitive than nLightR... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...and sorting. Reference images can be viewed by clicking on the links in the "Agree" and "Disagree" columns... repository for reagents, protocols, panels, publications, software, and datasets. It’s also a great place... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...swimming behavior, spontaneous swim or escape, was elicited when these different neuron groups were activated...illuminated. By looking at groups of neurons that elicited similar tail movements, they were able to narrow... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could have a major...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene. CRISPR-Act3.0, ... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post... a Cas9 nickase and two gRNAs. For nickase applications, a common question is: how should the gRNAs ...information and a link to the archived webinar, click here. We would like to thank IDT scientists Mollie... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...promoters, selectable markers, tags, origins of replication, and fluorescent proteins that are already included...the plasmid sequence with one or more enzymes. Click here to learn how to run an agarose gel We’... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...contain splice acceptors. They require insertion into an intron of a gene, where they can be spliced into... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...is resistant to specific compounds, links to publication references, and cross-references with cell line...manually going through the pdfs of the 26,000 publications cited. If the information seems incomplete or... -
Build Your CRISPR Vocabulary
TypeBlog Post...Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable... -
Fostering communication in remote collaborations
TypeBlog Post...meetings as opposed to phone calls most closely replicates how you’d interact with team members in person...access data and drafts of manuscripts and grant applications in real-time so they can provide input at the... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...takes minutes. As a result, it is not possible to elicit an immediate response or track the exact time of...beneficial for use in therapeutic or behavioral applications where drawn out effects are required. Targeted... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post... mutations in single stranded DNA undergoing replication. DNase on the other hand can digest and degrade...directly use your DNA preparation in any downstream application involving enzymatic reactions, a valid alternative... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog Post...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...