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  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...with the newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR... genotyping assay (such as Sanger sequencing). Click here for a useful reference.  More FAQs, CRISPR ...

  2. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ... perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...

  5. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in...Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...

  6. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR...

  8. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...these tools, which aren’t always easy to find in publications, so this is a much-needed community resource...exactly which strain you used so if they wish to replicate your experiment, they know exactly where to get...

  10. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...distributing new types of materials. At the time of publication we’ve already fulfilled more than 140 orders ...

  11. Plasmid Cloning by PCR

    Type
    Blog Post
    ...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly...

  12. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...

  13. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ...

  15. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ... we found in our map have an impact on virus replication. While we and our collaborators are starting ...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with...

  17. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...you will have. When possible, use low-bind or siliconized tubes to store samples as it is more difficult...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

  18. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

  20. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...of Toronto. He is an author of 20+ scientific publications and patents.   ...
Showing: 541 - 560 of 836 results