We narrowed to 874 results for: lic
-
TypeBlog Post... a Cas9 nickase and two gRNAs. For nickase applications, a common question is: how should the gRNAs ...information and a link to the archived webinar, click here. We would like to thank IDT scientists Mollie...
-
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...promoters, selectable markers, tags, origins of replication, and fluorescent proteins that are already included...the plasmid sequence with one or more enzymes. Click here to learn how to run an agarose gel We’... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...swimming behavior, spontaneous swim or escape, was elicited when these different neuron groups were activated...illuminated. By looking at groups of neurons that elicited similar tail movements, they were able to narrow... -
Build Your CRISPR Vocabulary
TypeBlog Post...Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...parasitization of cereal crops. As a result, agricultural applications of these novel biosensors could have a major...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene. CRISPR-Act3.0, ... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...contain splice acceptors. They require insertion into an intron of a gene, where they can be spliced into... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...is resistant to specific compounds, links to publication references, and cross-references with cell line...manually going through the pdfs of the 26,000 publications cited. If the information seems incomplete or... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog Post...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post... perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog Post...at Addgene have gone virtual! 2. Practice public speaking For almost any science career, there will... will be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...these tools, which aren’t always easy to find in publications, so this is a much-needed community resource...exactly which strain you used so if they wish to replicate your experiment, they know exactly where to get... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...cells, and has potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature... quantifying multiple gene editing activities. Click here to find the ACE reporter plasmid! Martin et... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...biological concepts - and you have yourself a plant publicity crisis. The Arabidopsis Biological Resource Center...Ohio State University. In her work, she focuses in public engagement in science and promoting plant science... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...reached the level of expression needed for most applications. Figure 1: With blue light, the VVD...Ventura. BLADE activates transcription with the flick of a light switch and shuts off just as quickly.... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in...Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...distributing new types of materials. At the time of publication we’ve already fulfilled more than 140 orders ... -
Plasmid Cloning by PCR
TypeBlog Post...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post... we found in our map have an impact on virus replication. While we and our collaborators are starting ...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with...